“…In this study, a variation between clones and light sources was observed in the in vitro introduction. Data found in the literature were similar to these results, showing that the combination of red/blue LEDs induced a higher number of shoots per explant in Dendrobium officinale and Ajuga multiflora (Li et al, 2013;Jeong and Sivanesan, 2018). These authors reported that LEDs are an effective alternative, especially during the in vitro regeneration of shoots.…”
Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.
“…In this study, a variation between clones and light sources was observed in the in vitro introduction. Data found in the literature were similar to these results, showing that the combination of red/blue LEDs induced a higher number of shoots per explant in Dendrobium officinale and Ajuga multiflora (Li et al, 2013;Jeong and Sivanesan, 2018). These authors reported that LEDs are an effective alternative, especially during the in vitro regeneration of shoots.…”
Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.
“…Higher the total protein content means higher expression and transcription of genes to run and process the signal perceived by cell. In literature the soluble protein content varies with the type of plant species, and certain generalization hard to make that certain light will lead to higher protein and MDA level [11,[37], [38], [39]]. Fig.…”
HighlightsCombination of thidiazuron and naphthalene acetic acid induced callus growth in Withania somnifera.Red light improved callus growth with lower antioxidant enzymes activities.Violet light enhanced the total phenolic and flavonoid content in callus culture of W. somnifera.Withaferin A and chlorogenic acid were detected in callus cultures.
“…This method has also been employed in several biotechnological applications including the production of bioactive compounds [1]. In recent years, several bioactive compounds like alkaloids, anthocyanins, carotenoids, fatty acids, flavonoids, phenolic acids, and tocopherols have been obtained from in vitro-developed shoots [2,3,4,5,6,7]. The synthesis and accumulation of specific bioactive compounds in shoot cultures depend largely on the composition of culture media and the environmental conditions of the culture [4,6].…”
Section: Introductionmentioning
confidence: 99%
“…In recent years, several bioactive compounds like alkaloids, anthocyanins, carotenoids, fatty acids, flavonoids, phenolic acids, and tocopherols have been obtained from in vitro-developed shoots [2,3,4,5,6,7]. The synthesis and accumulation of specific bioactive compounds in shoot cultures depend largely on the composition of culture media and the environmental conditions of the culture [4,6]. Carotenoids, flavonoids, phenolic acids and tocopherols are potent antioxidants, and are reported to possess numerous additional biological activities [8,9].…”
The goal of this study was to establish an efficient protocol for the large-scale propagation of Mertensia maritima (L.) Gray, and evaluate the carotenoid, fatty acid, and tocopherol contents in the leaves of in vitro regenerated shoots. Surface-disinfected node and shoot tip explants were placed on semisolid Murashige and Skoog (MS) medium with 0–16 µM N6-benzyladenine (BA), kinetin, (KN), and thidiazuron (TDZ) alone, or in combination with, 1 or 2 µM α-naphthaleneacetic acid (NAA). Of the three different cytokinins employed, TDZ elicited the best results for axillary shoot proliferation. A maximum frequency of shoot initiation above 84%, with a mean of 8.9 and 4.8 shoots per node and shoot tip, respectively, was achieved on the culture medium supplemented with 4 µM TDZ. A combination of TDZ + NAA significantly increased the percentage of multiple shoot formation and number of shoots per explant. The best shoot induction response occurred on MS medium with 4 µM TDZ and 1 µM NAA. On this medium, the node (93.8%) and shoot tip (95.9%) explants produced an average of 17.7 and 8.6 shoots, respectively. The highest root induction frequency (97.4%) and number of roots per shoot (25.4), as well as the greatest root length (4.2 cm), were obtained on half-strength MS medium supplemented with 4 µM indole-3-butyric acid (IBA). The presence of six carotenoids and α-tocopherol in the leaf tissues of M. maritima was confirmed by HPLC. Gas chromatography-mass spectrometry analysis confirmed the presence of 10 fatty acids, including γ-linolenic acid and stearidonic acid in the leaf tissues of M. maritima. All-E-lutein (18.49 μg g−1 fresh weight, FW), α-tocopherol (3.82 μg g−1 FW) and α-linolenic acid (30.37%) were found to be the significant compounds in M. maritima. For the first time, a successful protocol has been established for the mass propagation of M. maritima with promising prospects for harnessing its bioactive reserves.
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