Impact of long-term salinity exposure in anaerobic membrane bioreactors treating phenolic wastewater: Performance robustness and endured microbial community

Sierra, Julian Muñoz; Oosterkamp, Margreet J.; Wang, Wei; Spanjers, Henri; Lier, Jules B. van

doi:10.1016/j.watres.2018.05.006

Cited by 92 publications

(20 citation statements)

References 56 publications

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“…We pre-mixed the polymer with the inoculum, in 1 L jars of a jar-test apparatus by mixing at 90 rpm during 30 min. We filled each SMA bottle with 2.5 gCOD L −1 of sodium acetate, inoculum-polymer mixture, 0.6 mL L −1 micro and 6 mL L −1 macro nutrients solutions (Muñoz Sierra et al, 2018), 10 mM phosphate buffer solution at pH 7.0 (Spanjers and Vanrolleghem, 2016) and demineralized water, and then flushed the bottles with nitrogen gas for 1 min. The inoculum concentration in the bottles was 4 gVSS L −1 (corresponding to 6 gTSS L −1 ), and we used the following concentrations of polymer: 0, 0.06, 0.11, 0.17, 0.23, 0.28, 0.34, 0.40, and 0.46 gCOD L −1 .…”

Section: Methodsmentioning

confidence: 99%

Identification of the Methanogenesis Inhibition Mechanism Using Comparative Analysis of Mathematical Models

Odriozola

Abraham

Lousada-Ferreira

et al. 2019

Front. Bioeng. Biotechnol.

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The application of cationic polymers to enhance membrane fluxes in anaerobic membrane bioreactors has been proposed by several authors. However, literature shows contradictory results on the influence of those chemicals on the biological activity. In this research, we studied the effect of a cationic polymer on the production of methane from acetate by acetoclastic methanogens. We assessed the effect of polymer concentration on the accumulated methane production (AMP) and the specific methanogenic activity (SMA) in batch tests. Batch tests results showed lower SMA values at higher concentrations of polymer and no effect on the final AMP. Different inhibition models were calibrated and compared to find the best fit and to hypothesize the prevailing inhibition mechanisms. The assessed inhibition models were: competitive (M1a), non-competitive (M2a), un-competitive (M3a), biocide-linear (M4a), and biocide-exponential (M5a). The parameters in the model related to the polymer characteristics were adjusted to fit the experimental data. M2a and M3a were the only models that fitted both experimental SMA and AMP. Although M1a and M4a adequately fitted the experimental SMA, M1a simulations slightly deviated from the experimental AMP, and M4a considerably underpredicted the AMP at concentrations of polymer above 0.23 gCOD L −1 . M5a did not adequately fit either experimental SMA and AMP results. We compared models a (M1a to M5a), which consider the inhibition by the concentration of polymer in the bulk liquid, with models b (M1b to M5b) considering the inhibition being caused by the total concentration of polymer in the reactor. Results showed that the difference between a and b models' simulations were negligible for all kinetic models considered (M1, M2, M3, M4, and M5). Therefore, the models that better predicted the experimental data were the non-competitive (M2a and M2b) and un-competitive (M3a and M3b) inhibition models, which are biostatic inhibition models. Consequently, the decreased methanogenic activity caused by polymer additions is presumably a reversible process

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Section: Methodsmentioning

confidence: 99%

Identification of the Methanogenesis Inhibition Mechanism Using Comparative Analysis of Mathematical Models

Odriozola

Abraham

Lousada-Ferreira

et al. 2019

Front. Bioeng. Biotechnol.

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“…For the pre-activation period, all the SMA bottles, including blanks, were filled with 1.0 gCOD L −1 of sodium acetate, 2 gVSS L −1 of inoculum, 0.6 mL L −1 micro and 6 mL L −1 macro nutrients solutions [ 28 , 29 ], 10 mM phosphate buffer solution at pH 7.0 [ 30 ] and demineralized water. The bottles were flushed with nitrogen gas for 1 min and placed inside an orbital shaker at 130 rpm with temperature set at 35 °C.…”

Section: Methodsmentioning

confidence: 99%

Fouling Mitigation by Cationic Polymer Addition into a Pilot-Scale Anaerobic Membrane Bioreactor Fed with Blackwater

Odriozola

Morales²,

Vázquez-Padín³

et al. 2020

Polymers

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Cationic polymers have proven to be suitable flux enhancers (FEs) in large-scale aerobic membrane bioreactors (MBRs), whereas in anaerobic membrane bioreactors (AnMBRs) research is scarce, and so far, only done at lab‐scale. Results from MBRs cannot be directly translated to AnMBRs because the extent and nature of membrane fouling under anaerobic and aerobic conditions are different. Our research focused on the long-term effect of dosing the cationic polymer Adifloc KD451 to a pilot AnMBR, fed with source‐separated domestic blackwater. A single dosage of Adifloc KD451 at 50 mg L−1 significantly enhanced the filtration performance in the AnMBR, revealed by a decrease in both fouling rate and total filtration resistance. Nevertheless, FE addition had an immediate negative effect on the specific methanogenic activity (SMA), but this was a reversible process that had no adverse effect on permeate quality or chemical oxygen demand (COD) removal in the AnMBR. Moreover, the FE had a long‐term positive effect on AnMBR filtration performance and sludge filterability. These findings indicate that dosing Adifloc KD451 is a suitable strategy for fouling mitigation in AnMBRs because it led to a long‐term improvement in filtration performance, while having no significant adverse effects on permeate quality or COD removal.

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“…This bacteria has been enriched under anaerobic thermophilic conditions from an oil reservoir containing mostly halophilic species (Dellagnezze et al, 2016). Similarly, high salinity conditions will enrich for salt-tolerant and halophilic Thermovirga and Clostridium species (Muñoz Sierra et al, 2018a). Acetomicrobium hydrogeniformans sp., which also increased during phase III, is found in oil production water and is capable of producing hydrogen.…”

Section: Microbial Community Structurementioning

confidence: 99%

Anaerobic Conversion of Saline Phenol-Containing Wastewater Under Thermophilic Conditions in a Membrane Bioreactor

Sierra

Rea

Cerqueda-García

et al. 2020

Front. Bioeng. Biotechnol.

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Closing water loops in chemical industries result in hot and highly saline residual streams, often characterized by high strength and the presence of refractory or toxic compounds. These streams are attractive for anaerobic technologies, provided the chemical compounds are biodegradable. However, under such harsh conditions, effective biomass immobilization is difficult, limiting the use of the commonly applied sludge bed reactors. In this study, we assessed the long-term phenol conversion capacity of a lab-scale anaerobic membrane bioreactor (AnMBR) operated at 55 • C, and high salinity (18 gNa +. L −1). Over 388 days, bioreactor performance and microbial community dynamics were monitored using specific methanogenic activity (SMA) assays, phenol conversion rate assays, volatile fatty acids permeate characterization and Illumina MiSeq analysis of 16S rRNA gene sequences. Phenol accumulation to concentrations exceeding 600 mgPh. L −1 in the reactor significantly reduced methanogenesis at different phases of operation, while applying a phenol volumetric loading rate of 0.12 gPh. L −1. d −1. Stable AnMBR reactor performance could be attained by applying a sludge phenol loading rate of about 20 mgPh. gVSS −1. d −1. In situ maximum phenol conversion rates of 21.3 mgPh. gVSS −1. d −1 were achieved, whereas conversion rates of 32.8 mgPh. gVSS −1. d −1 were assessed in ex situ batch tests at the end of the operation. The absence of caproate as intermediate inferred that the phenol conversion pathway likely occurred via carboxylation to benzoate. Strikingly, the hydrogenotrophic SMA of 0.34 gCOD-CH 4. gVSS −1. d −1 of the AnMBR biomass significantly exceeded the acetotrophic SMA, which only reached 0.15 gCOD-CH 4. gVSS −1. d −1. Our results indicated that during the course of the experiment, acetate conversion gradually changed from acetoclastic methanogenesis to acetate oxidation coupled to hydrogenotrophic methanogenesis. Correspondingly, hydrogenotrophic methanogens of the class Methanomicrobia, together with Synergistia, Thermotogae, and Clostridia classes, dominated the microbial community and were enriched during the three phases of operation, while the aceticlastic Methanosaeta species remarkably decreased. Our findings clearly showed that highly saline phenolic wastewaters could be satisfactorily

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Impact of long-term salinity exposure in anaerobic membrane bioreactors treating phenolic wastewater: Performance robustness and endured microbial community

Cited by 92 publications

References 56 publications

Identification of the Methanogenesis Inhibition Mechanism Using Comparative Analysis of Mathematical Models

Identification of the Methanogenesis Inhibition Mechanism Using Comparative Analysis of Mathematical Models

Fouling Mitigation by Cationic Polymer Addition into a Pilot-Scale Anaerobic Membrane Bioreactor Fed with Blackwater

Anaerobic Conversion of Saline Phenol-Containing Wastewater Under Thermophilic Conditions in a Membrane Bioreactor

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