2003
DOI: 10.1021/bp025665e
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Impact of Low-Temperature Plasmas on Deinococcus radiodurans and Biomolecules

Abstract: The effects of cold plasma on Deinococcus radiodurans, plasmid DNA, and model proteins were assessed using microbiological, spectrometric, and biochemical techniques. In low power O(2) plasma (approximately 25 W, approximately 45 mTorr, 90 min), D. radiodurans, a radiation-resistant bacterium, showed a 99.999% reduction in bioburden. In higher power O(2) plasma (100 W and 500 mTorr), the reduction rate increased about 10-fold and observation by atomic force microscopy showed significant damage to the cell. Dam… Show more

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Cited by 75 publications
(73 citation statements)
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“…[4][5][6] In contrast, our results show only a weak (below 10%) multifragmentation of DNA, which is observed as smeared bands in the gel image.…”
mentioning
confidence: 55%
“…[4][5][6] In contrast, our results show only a weak (below 10%) multifragmentation of DNA, which is observed as smeared bands in the gel image.…”
mentioning
confidence: 55%
“…Low-pressure oxygen plasma has been shown to degrade lipids, proteins and DNA of cells (Mogul et al 2003). The reactive species in plasma have been widely associated to the direct oxidative effects on the outer surface of microbial cells.…”
Section: Action On Cell Components and Functionsmentioning
confidence: 99%
“…Many groups already demonstrated that plasma treatment can lead, under appropriate operational conditions, to fast sterilisation of different strains of bacteria or bacterial spores, [10][11][12][13][14] depyrogenation of bacterial endotoxins, [14][15][16][17][18] or to efficient elimination of protein residuals from surfaces. [14,[19][20][21][22][23][24][25] Plasma treatment of surfaces as a sterilisation or decontamination method is a promising approach to overcome limitations of conventional techniques. The precise characterisation of the employed plasma discharges, the application of sensitive surface diagnostic methods and targeted experiments to separate the effects of different agents, have led to rapid progress in the understanding of different relevant elementary processes.…”
Section: Introductionmentioning
confidence: 99%