Impact of &lt;i&gt;Achyranthes aspera&lt;/i&gt; Leaf and Stem Extracts on the Survival, Morphology and Behaviour of an Indian Strain of Dengue Vector, &lt;i&gt;Aedes aegypti&lt;/i&gt; L. (Diptera: Culicidae)

Sharma, Aarti; Kumar, Sarita; Tripathi, Pushplata

doi:10.5376/jmr.2015.05.0007

Cited by 15 publications

(25 citation statements)

References 17 publications

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“…Larvae treated with 8 ppm bruceine A exhibited completely diffuse necrotic gastrointestinal epithelial cells which completely detached from the basement membrane and localized in the lumen. These results are consistent with that reported by Sharma et al [18] who stated that midgut epithelial cells exhibited intense damage at 6, 12, 24, and 48 h after treatment with A. aspera extract. Highly typical changes include the vacuolization of midgut columnar cells, damage to microvilli, the release of epithelial cell content into the midgut lumen, and eventual cell death.…”

Section: Histological Changes Of a Aegypti (L) Larvaesupporting

confidence: 93%

“…In addition, respiratory tubes, siphon, and anal gills were having more severe damage. These results are similar to those observed by Sharma et al [18], who reported that the larvae of A. aegypti exhibited distorted midguts, pigmentation loss, and partial or total cell damage after treatment with extracts from the stems and leaves of A. aspera. Digestive tract damage was more visible in larvae treated with the hexane extract of A. aspera leaves than those treated with extracts from A. aspera stems.…”

Section: Morphological Changes Of a Aegypti (L) Larvaesupporting

confidence: 91%

“…Morphological tests were conducted in accordance with the method reported by Sharma et al [18] with slight modifications. A. aegypti (L.) larvae in instar III to the beginning of instar IV were placed in glass jars, each containing 199 mL of water and 1 mL of bruceine A at various LC or 1 ppm of the positive control temefos.…”

Section: Morphological Testmentioning

confidence: 99%

“…The research of Warikoo and Kumar [22] reported that treatment with excess Argemone mexicana damaged the anal papillae of A. aegypti larvae. Sharma et al [18] showed that treatment with extracts of the stems and leaves of Achyranthes aspera caused a structural damage to the anal papillae of larvae of A. aegypti in the early fourth instar. In the present study, microscopic observations showed that the internal membranes of anal papillae were shrank, whereas external membranes were remained normal.…”

Section: Morphological Changes Of a Aegypti (L) Larvaementioning

confidence: 99%

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Morphological and Histological Effects of Bruceine a on the Larvae of Aedes Aegypti Linnaeus (Diptera: Culicidae)

Sutiningsih

Mustofa

Satoto

et al. 2018

Asian J Pharm Clin Res

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Objective: This study aimed to determine a target of action of bruceine A on the basis of its morphological and histological effects on the larvae of Aedes aegypti Linnaeus.Methods: Bruceine A was isolated from Brucea javanica (L.) Merr. seeds in accordance with the Mangungsong method. Larvae of A. Aegypti (L.) in instar III to the beginning of instar IV were treated with various concentrations of bruceine A. The negative control group did not receive any treatment, whereas the positive control group received 1 ppm temefos. Dead larvae were collected after 24 h of treatment for the examination of morphological and histological changes.Results: The negative control group did not exhibit any morphological and histological changes. Larvae treated with bruceine A, however, had visible damaged heads, cuticles, digestive and respiration tracts, respiratory siphons, and setae, and they were smaller than normal larvae. Larvae treated with temefos exhibited gastrointestinal damage, narrowed breathing tubes, cuticle damage, and detached/damaged seta feathers. The necrosis of gastrointestinal epithelial cells was the major histological change exhibited by larvae treated with various concentrations of bruceine A or 1 ppm temefos.Conclusion: The targets of action of bruceine A in A. aegypti (L.) larvae are the head/caput, cuticle, setae, siphon, and gastrointestinal and respiratory tracts.

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Section: Histological Changes Of a Aegypti (L) Larvaesupporting

confidence: 93%

Section: Morphological Changes Of a Aegypti (L) Larvaesupporting

confidence: 91%

Section: Morphological Testmentioning

confidence: 99%

Section: Morphological Changes Of a Aegypti (L) Larvaementioning

confidence: 99%

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Morphological and Histological Effects of Bruceine a on the Larvae of Aedes Aegypti Linnaeus (Diptera: Culicidae)

Sutiningsih

Mustofa

Satoto

et al. 2018

Asian J Pharm Clin Res

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“…This means that bruceine A is effective as a natural larvicide on the larvae of A. aegypti, which is in accordance with the provisions of the Pesticide Committee (1995). According to Sharma et al (2015), the interaction of toxic substances in a biological system is determined by the concentration and duration of the test. One-way ANOVA test results showed that the average number of deaths of A. aegypti larvae at various concentrations of bruceine A was significantly different (p<0.05).…”

Section: Larvicidal Activity Of Bruceine a Againts A Aegyptimentioning

confidence: 99%

Larvicidal Activity of Bruceine A against Aedes aegypti and Toxicity on Vero Cells

Sutiningsih¹,

Mustofa²,

Satoto³

et al. 2018

OnLine Journal of Biological Sciences

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Vector control is still based on the use of chemical insecticides, which can cause death of nontarget animals, pollution and the emergence of vector resistance. This study aims to assess the larvicidal activity of bruceine A against larvae of Aedes aegypti and its cytotoxic activity against Vero cells. Extraction and isolation of bruceine A from the seeds of Brucea javanica (L.) Merr by method of Subeki. The purity of bruceine A isolate is determined by using a thin layer of chromatography and high performance liquid chromatography. Larvicidal activity of bruceine A on the larvae of A. aegypti from instar III until the beginning of instar IV was measured using a bioassay method. The examination of bruceine A cytotoxicity on Vero cells was performed by Micro-culture Tetrazolium assay (MTT). The results showed that mortality of A. aegypti larvae increased with increasing concentration of bruceine A. Log probit analysis of the larva mortality showed that the lethal concentration 50 and 90 (LC 50, LC 90) were 0.453±0.022 ppm and 4.962±0.681 ppm for 24 h respectively. The cytotoxic activity of bruceine A in Vero cells is low, with inhibitor concentration 50 (IC 50) values of 1251.324±0.162 µg/mL. Bruceine A has larvicidal activity against A. aegypti; therefore, it is a potential natural larvicide with low cytotoxicity.

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Design, synthesis, characterization and insecticidal screening of novel anthranilic diamides comprising acyl thiourea substructure

Kavyasri

Sundharesan

Mathew

2022

Pest Management Science

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Background Mosquito‐borne pathogens constitute a major health problem worldwide. The extermination of the mosquito remains a significant issue in public health. Chemical insecticides have been used to control mosquitoes for decades. However, resistance has become a limiting factor for their control. The anthranilic diamide insecticides possess excellent insecticidal activities against Lepidoptera and its resistant strains by draining internal calcium stores on activating insect ryanodine receptors. However, the reports on the effect on mosquitoes are scarce and hence a series of novel anthranilic diamides comprising acyl thiourea substructure were synthesized and their insecticidal activities against three vector mosquito larvae namely, Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi were evaluated as per WHO protocol. Also investigated the morphological observations of treated larvae. Results Novel anthranilic diamides containing an acyl thiourea substructure were synthesized and structures were established by 1H nuclear magnetic resonance (NMR), 13C NMR, Fourier transform infrared (FTIR) and high‐resolution mass spectrometry (HR‐MS). Mosquito larvicidal activity of the title compounds 6‐a–s revealed that compound 6‐l exhibited marked larvicidal activities against C. quinquefasciatus and A. aegypti 3rd instar larvae with median lethal concentrations (LC50) values of 0.0044 mm and 0.0070 mm, respectively, for 48 hours of treatment. Compound 6‐g exhibited larvicidal activity against An. stephensi with LC50 value of 0.0085 mm. Peculiar morphological alterations in the body of the treated larvae leading to death were observed on microscopic examination. Conclusion Novel anthranilic diamides containing an acyl thiourea substructure were designed, synthesized and characterized. Their bioassay results demonstrated significant mosquito larvicidal activity with striking morphological alterations in the body, which should ensure forthcoming designs of highly active diamide derivatives as mosquito larvicides. © 2022 Society of Chemical Industry.

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Impact of <i>Achyranthes aspera</i> Leaf and Stem Extracts on the Survival, Morphology and Behaviour of an Indian Strain of Dengue Vector, <i>Aedes aegypti</i> L. (Diptera: Culicidae)

Cited by 15 publications

References 17 publications

Morphological and Histological Effects of Bruceine a on the Larvae of Aedes Aegypti Linnaeus (Diptera: Culicidae)

Morphological and Histological Effects of Bruceine a on the Larvae of Aedes Aegypti Linnaeus (Diptera: Culicidae)

Larvicidal Activity of Bruceine A against Aedes aegypti and Toxicity on Vero Cells

Design, synthesis, characterization and insecticidal screening of novel anthranilic diamides comprising acyl thiourea substructure

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