Impact of Macular Pigment on Fundus Autofluorescence Lifetimes

Sauer, Lydia; Schweitzer, Dietrich; Ramm, Lisa; Augsten, R; Hammer, Martin; Peters, Sven

doi:10.1167/iovs.14-15335

Cited by 67 publications

(126 citation statements)

References 61 publications

(97 reference statements)

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“…On the other hand, short lifetimes within atrophic lesions were mainly seen within the central macular area. Given the recent observations by Sauer et al [23,31], which linked macular pigment to short lifetimes by correlating foveal macular pigment optical density with mean foveal fluorescence lifetimes, the shorter lifetimes we observed are likely to originate from macular pigment. Furthermore, differences between STGD and AMD seen in FLIO could possibly also be explained by an abnormal accumulation of lipofuscin.…”

Section: Discussionsupporting

confidence: 57%

“…The fluorescence lifetime imaging ophthalmoscope, based on a Heidelberg retina angiograph Spectralis system (Heidelberg Engineering, Heidelberg, Germany), was used to obtain fluorescence lifetime data. The principles as well as technical details and laser safety calculations behind FLIO have been described in previous publications [12,22,23].…”

Section: Fluorescence Lifetime Imaging Ophthalmoscopementioning

confidence: 99%

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Fluorescence Lifetime Patterns of Retinal Pigment Epithelium Atrophy in Patients with Stargardt Disease and Age-Related Macular Degeneration

et al. 2019

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Purpose: To investigate whether autofluorescence lifetime patterns within retinal pigment epithelium (RPE) atrophy differ between age-related macular degeneration (AMD) and Stargardt disease (STGD). Methods: Mean retinal autofluorescence lifetimes were measured in a short and a long spectral channel (SSC: 498-560 nm; LSC: 560-720 nm). Mean retinal fluorescence lifetimes were analyzed with corresponding clinical features, fundus images, fundus autofluorescence intensity images, and optical coherence tomography. Mean fluorescence lifetime values of atrophic areas were compared between the two cohorts and within the same patient to adjacent nonatrophic regions. Results: Mean fluorescence lifetimes within areas with RPE atrophy of 13 patients with STGD (mean age ± SEM 43.7 ± 5 years) and 30 patients with geographic atrophy (mean age: 78 ± 2 years) were analyzed and compared to age-matched healthy participants. The mean area of RPE atrophy in STGD and AMD was 6.6 ± 2.3 mm 2 (range: 0.66-33.17 mm 2 ) and 17.5 ± 3.8 mm 2 (range: 0.58-50.02 mm 2 ), respectively. In patients with AMD, atrophic areas revealed significantly longer mean fluorescence lifetime values as compared with patients with STGD (SSC: 997 ± 60 vs. 363 ± 26 ps; LSC: 880 ± 46 vs. 393 ± 23 ps; p < 0.0001). Conclusions: This study established that RPE atrophy in patients secondary to STGD and AMD display distinctive mean fluorescence lifetime characteristics. As retinal fluorescence lifetimes within areas of RPE atrophy were significantly longer in AMD patients, the analysis of specific lifetime patterns may provide additional insight into the disease processes and the pathogenetic mechanisms in the development of atrophic patches in AMD and STGD.

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Section: Discussionsupporting

confidence: 57%

Section: Fluorescence Lifetime Imaging Ophthalmoscopementioning

confidence: 99%

Fluorescence Lifetime Patterns of Retinal Pigment Epithelium Atrophy in Patients with Stargardt Disease and Age-Related Macular Degeneration

et al. 2019

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“…Xanthophylls in vitro show a fluorescence emission of approximately 500e580 nm and a peak at 520e540 nm (Sharifzadeh et al, 2006). Due to the peak emission wavelength, the SSC is more influenced by macular pigment fluorescence than the LSC, which is reflected in a stronger correlation for the SSC (Snyder et al, 1985;Cosgrove et al, 1990;Decoster et al, 1992;Andersson and Gillbro, 1995;Sauer et al, 2015).…”

Section: à4mentioning

confidence: 99%

Fluorescence lifetime imaging ophthalmoscopy

Dysli

Wolf

Zinkernagel

2017

Acta Ophthalmologica

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a b s t r a c tImaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores. It mainly derives its signal from lipofuscin at the level of the retinal pigment epithelium. Fundus autofluorescence, however, can not only be characterized by the spatial distribution of the fluorescence intensity or emission spectrum, but also by a characteristic fluorescence lifetime function. The fluorescence lifetime is the average amount of time a fluorophore remains in the excited state following excitation. Fluorescence lifetime imaging ophthalmoscopy (FLIO) is an emerging imaging modality for in vivo measurement of lifetimes of endogenous retinal fluorophores. Recent reports in this field have contributed to our understanding of the pathophysiology of various macular and retinal diseases.Within this review, the basic concept of fluorescence lifetime imaging is provided. It includes technical background information and correlation with in vitro measurements of individual retinal metabolites. In a second part, clinical applications of fluorescence lifetime imaging and fluorescence lifetime features of selected retinal diseases such as Stargardt disease, age-related macular degeneration, choroideremia, central serous chorioretinopathy, macular holes, diabetic retinopathy, and retinal artery occlusion are discussed. Potential areas of use for fluorescence lifetime imaging ophthalmoscopy will be outlined at the end of this review.

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“…The laser safety of FLIO has been described in detail elsewhere (Sauer et al. ). Briefly, a single laser pulse exposes the retina with an energy of 2.5 10 −12 J (laser class 1), which is approximately four orders of magnitude below the maximum permissible energy according to American National Standards Institute [ANSI Z136.1‐2000 (ANSI )].…”

Section: Subjectsmentioning

confidence: 99%