Impact of MALDI‐TOF MS in Clinical Mycology; Progress and Barriers in Diagnostics

Santos, Cledir; Francisco, Elaine Cristina; Mazza, Mariana; Padovan, Ana Carolina Barbosa; Colombo, Arnaldo Lopes; Lima, Nelson

doi:10.1002/9781118960226.ch9

Cited by 10 publications

(10 citation statements)

References 62 publications

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“…Identification of Fusarium genus by MALDI-TOF MS is not yet accurately investigated due to low number of representative taxa in databases [32,53]. Our study identified 12 different spectral mass patterns, from which six within the FSSC and four within the FOSC.…”

Section: Discussionmentioning

confidence: 93%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

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The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.

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Section: Discussionmentioning

confidence: 93%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

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“…A number of studies have reported that misidentification of organisms by MALDI-TOF MS appeared to have been caused by the incompleteness of the reference libraries used (2,(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26). Two types of reference libraries are currently in use in Canada: clinical libraries with spectra approved by Health Canada and research libraries, which contain a wider selection of spectra but are not cleared for clinical applications.…”

Section: Rapid Communicationmentioning

confidence: 99%

Misidentification of Risk Group 3/Security Sensitive Biological Agents by MALDI-TOF MS in Canada: November 2015–October 2017

Pomerleau-Normandin¹,

Heisz²,

Su³

2018

CCDR

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Background: Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) is a technology increasingly used in diagnostic identification of microorganisms. However, anecdotal evidence suggests that this technology is associated with misidentification of Risk Group 3 (RG3)/Security Sensitive Biological Agents (SSBA) resulting in exposure risks to laboratory personnel. Objective: To investigate and characterize incidents related to the use of MALDI-TOF MS in

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“…Subsequently, Seyfarth et al [27] and Erhard et al [28] published the first protocols for identification by MALDI-TOF MS of Fusarium proliferatum and dermatophytes, respectively. Santos et al [24] recently review the impact of MALDI-TOF MS in clinical mycology laboratories pointing out the main progresses and barriers in fungal identification. These authors elected as limitations the commercial libraries with limited coverage of representative fungal taxa and the requirement to standardise procedures.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a cost-effective alternative for bacteria, mycobacteria, and yeast identification [ 16 , 17 ]. For filamentous fungi, since the first reports published in 2000 by Welham et al [ 18 ] and Li et al [ 19 ], where spores were analysed, many other subsequent contributions discussed developments for their identification by MALDI-TOF MS [ 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Subsequently, Seyfarth et al [ 27 ] and Erhard et al [ 28 ] published the first protocols for identification by MALDI-TOF MS of Fusarium proliferatum and dermatophytes, respectively.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)

Gómez-Velásquez¹,

Loaiza-Díaz²,

Hernández

et al. 2020

Microorganisms

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Identification of filamentous fungi by conventional phenotypic methods are time-consuming, and a correct identification at the species level is prone to errors. Therefore, a more accurate and faster time-to-results, and cost-effective technique, is required, such as the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). In this study, we describe the development of an in-house spectra library for the identification of filamentous fungi frequently isolated from patients with infections. An in-house spectra library was constructed using 14 reference strains grown in solid medium. Clinical isolates were identified either by the in-house spectra library or the Biotyper commercial library from Bruker Daltonics. Fungal identification was carried following the Biotyper’s established scores: ≤1.699: not reliably identified (NRI); 1.700–1.999: genus-level; ≥2.000: species-level. Clinical isolates were identified, with the in-house library, at species- and genus-level at 88.70% (55) and 3.22% (2), respectively. While 4.80% (3) was NRI and 3.22% (2) was discrepant concerning sequencing. On the contrary, identification up to species and genus-level with the commercial library was 44.44% (16) and 22.22% (8), respectively. NRI and the discrepancy was 30.55% (11) and 2.77% (1), respectively. For the reaming 26 isolates, 16 from Neoscytalidium dimidiatum and 10 from Sporothrix spp., respectively, the absence of spectrum and the specific spectra within the Sporothrix complex in the commercial library resulted in the inability to obtain an identification. In conclusion, the current results advocate the importance that each clinical microbiological laboratory needs to develop an ad hoc library associated with the MALDI-TOF MS fungal identification to overcome the limitations of the available commercial libraries.

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Impact of MALDI‐TOF MS in Clinical Mycology; Progress and Barriers in Diagnostics

Cited by 10 publications

References 62 publications

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Misidentification of Risk Group 3/Security Sensitive Biological Agents by MALDI-TOF MS in Canada: November 2015–October 2017

Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)

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