Impact of maternal and neonatal factors on umbilical cord CD34+ cells

Abstract

Background: The achievement of optimal number of CD34 + umbilical cord stem cells is essential for successful umbilical cord stem cell transplantation. So the aim of this study was to assess the potential effect of both maternal and neonatal factors on the umbilical cord blood CD34 + cell count. Methods:The study was done on umbilical cord blood samples obtained from 20 mothers during labor.Their ages ranged from 22 to 34 years and were subjected to history taking, physical examination of the baby and assessment of the CD34 + cells count in umbilical cord blood.Results: Number of previous live births and weight of the baby had a significant effect on CD34 + cells count while the sex of the baby, delivery route, maternal age and gestation period had no significant effect on CD34 + cells count.Conclusions: Umbilical cord blood-derived CD34 + cell count is better with good weight and first babies and decreased with subsequent babies.

Metabolic sensor O-GlcNAcylation regulates erythroid differentiation and globin production via BCL11A

“…Due to limitations in cell number and case-to-case variations of CD34 + -derived erythroid cells, which were likely the influence of maternal and neonatal factors, e.g., maternal age, gestational age, birth order, birth weight, infant sex, and length of umbilical cord [ 32 , 33 ], human-derived erythroblastic leukemia K562 cells were used to further investigate the regulatory mechanisms behind terminal erythroid differentiation, which theoretically starts from proerythroblasts that proliferate and differentiate to generate reticulocytes [ 22 ]. We first optimized the differentiation protocol to obtain healthy cells in all stages and a sufficient amount of hemoglobin for protein analysis by modifying the cell density, concentrations, and duration of hemin used by Uchida et al (Protocol 0) [ 19 ] as well as by employing NaB to induce erythroid maturation and enucleation, as schematically depicted in Fig.…”

Mild or Moderate COVID-19 during Pregnancy Does Not Affect the Content of CD34+ Hematopoietic Stem Cells in Umbilical Cord Blood of Newborns

“…Over the past few decades, UCB has been regarded as a viable graft source and an effective alternative to bone marrow transplantation [13,17]. It is not surprising that the main attention of researchers was paid to predictors of obtaining the largest amount of both the UCB itself and the leukocytes including various stem/progenitor cell populations [24,36]. The factors that significantly affect these parameters can include the duration of pregnancy and body weight of the newborn.…”

Impact of Intrauterine Growth Restriction Diseases on The Umbilical Cord Blood CD34+ Cell Counts

“…Many of the previous reports revealed a positive correlation between birth weight and CD34+ cells count. [9][10][11]16 In light of divergent results from several studies, differences between groups should be taken into account when evaluating the results.…”

“…[6][7][8] Because of these restrictive problems, research in the literature has focused on factors altering CD34+ cells product such as maternal age, smoking status, birth weight, gestational age, gender, mode of delivery, preeclampsia. [9][10][11][12] Whereas the effect of morbidly adherent placenta (MAP) disease caused by abnormal implantation of the basal plate, 13 reported as 3% in women with previous cesarean history and 60% in women with three previous cesarean histories, MAP's impact on CD34+ cells 14 has not been demonstrated. 15 Also, there are not many articles on the effect of gestational diabetes mellitus (GDM) and gestational hypertension (GHT) diseases on the number of CD34+ cells in the umbilical cord blood, which are important diseases in obstetrics and gynecology practice.…”