Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics

Ji, Chenglong; Yu, Deliang; Wang, Qing; Li, Fēi; Zhao, Jianmin; Wu, Huifeng

doi:10.1016/j.marpolbul.2016.02.052

Cited by 22 publications

(22 citation statements)

References 14 publications

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“…In the mussels collected from the Bohai Sea in China, a survey reported that the mussels from some of the sampling sites were polluted by Cd and As with average concentrations at~2 and 5 mg/L, respectively [2]. In our recent study, the Cd and As pollution have been confirmed by the high accumulations of Cd and As in marine shrimp Crangon affinis collected from the Yellow River Estuary and Laizhou Bay along the Bohai coast, respectively [3]. Although Cd is relatively rare in marine environment, the concentration of Cd has reached 50 mg/L in some heavily polluted estuaries or harbors and ports [4].…”

Section: Introductionmentioning

confidence: 72%

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

et al. 2016

Fish & Shellfish Immunology

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a b s t r a c tCadmium (Cd) and arsenic (As) are the main metal/metalloid contaminants in the coastal environments of the Bohai Sea, China. In this work, a combined proteomic and metabolomic approach was applied to investigate the biological effects of Cd and As (V) in the early life stage (D-shape larvae) of mussel Mytilus galloprovincialis. Results indicated that Cd was a potential immune toxicant to D-shape larval mussel because of the numerous proteomic responses related to immune system. Additionally, Cd induced oxidative stress, cellular injury and disturbance in nucleic acid metabolism in D-shape larval mussels. However, only two identified proteins were significantly altered in As (V)-treated group, suggesting that D-shape larval mussel was less sensitive to As (V) than to Cd at protein level. These two proteins in response to As (V) suggested that As (V) influenced anti-oxidative system and cell proliferation in Dshape larval mussels. Metabolic responses indicated that Cd and As (V) induced disturbances in osmotic regulation and energy metabolism in D-shape larval mussels via different metabolic pathways. In addition, Cd reduced lipid metabolism as well. This work demonstrated that a combination of proteomics and metabolomics could provide an insightful view in the biological effects of pollutants in mussel M. galloprovincialis at an early life stage.

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Section: Introductionmentioning

confidence: 72%

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

et al. 2016

Fish & Shellfish Immunology

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“…Sun et al reported that Cd posed prominent risk on the marine organisms and ecosystem due to the high concentrations of Cd in the sediments from the Bohai coast [1]. Our recent research indicated that the dominant species, shrimp Crangon affinis, was contaminated by Cd from the Yellow River Estuary along the Bohai Sea [2]. As it is known, Cd can induce multiple adverse effects in organisms.…”

Section: Introductionmentioning

confidence: 99%

Proteomic responses reveal the differential effects induced by cadmium in mussels Mytilus galloprovincialis at early life stages

Peng

et al. 2016

Fish & Shellfish Immunology

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a b s t r a c tCadmium (Cd) has become an important metal contaminant and posed severe risk on the organisms in the coastal environments of the Bohai Sea. Marine mussel Mytilus galloprovincialis is widely distributed along the Bohai coast and consumed as seafood by local residents. Evidences indicate that the early stages of marine organisms are more sensitive to metal contaminants. In this study, we applied twodimensional electrophoresis-based proteomics to characterize the biological effects of Cd (50 mg L À1 ) in the early life stages (D-shape larval and juvenile) of mussels. The different proteomic responses demonstrated the differential responsive mechanisms to Cd exposure in these two early life stages of mussels. In details, results indicated that Cd mainly induced immune and oxidative stresses in both Dshape larval and juvenile mussels via different pathways. In addition, the significant up-regulation of triosephosphate isomerase and metallothionein confirmed the enhanced energy demand and mobilized detoxification mechanism in D-shape larval mussels exposed to Cd. In juvenile mussels, Cd exposure also induced clear apoptosis. Overall, this work suggests that Cd is a potential immune toxicant to mussel M. galloprovincialis at early life stages.

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“…Recent studies revealed that chronic As exposure perturbed amino acids metabolism . Decreased level of tyrosine caused by As exposure were reported in serum of rats, and in tissues of marine animals, and that tyrosine can be biomarker of As contamination in shrimp Crangon affinis . However, similarly, our previous study showed that tyrosine augmented in cerebral cortex after 12‐week As exposure, and that there were differences between cerebral cortex and serum metabolomics .…”

Section: Disscusionmentioning

confidence: 59%

Gas chromatography‐mass spectrometry based metabolomics profile of hippocampus and cerebellum in mice after chronic arsenic exposure

Hua

Du³

et al. 2018

Environmental Toxicology

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Intake of arsenic (As) via drinking water has been a serious threat to global public health. Though there are numerous reports of As neurotoxicity, its pathogenesis mechanisms remain vague especially its chronic effects on metabolic network. Hippocampus is a renowned area in relation to learning and memory, whilst recently, cerebellum is argued to be involved with process of cognition. Therefore, the study aimed to explore metabolomics alternations in these two areas after chronic As exposure, with the purpose of further illustrating details of As neurotoxicity.Twelve 3-week-old male C57BL/6J mice were divided into two groups, receiving deionized drinking water (control group) or 50 mg/L of sodium arsenite (via drinking water) for 24 weeks.Learning and memory abilities were tested by Morris water maze (MWM) test. Pathological and morphological changes of hippocampus and cerebellum were captured via transmission electron microscopy (TEM). Metabolic alterations were analyzed by gas chromatography-mass spectrometry (GC-MS). MWM test confirmed impairments of learning and memory abilities of mice after chronic As exposure. Metabolomics identifications indicated that tyrosine increased and aspartic acid (Asp) decreased simultaneously in both hippocampus and cerebellum. Intermediates (succinic acid) and indirect involved components of tricarboxylic acid cycle (proline, cysteine, and alanine) were found declined in cerebellum, indicating disordered energy metabolism. Our findings suggest that these metabolite alterations are related to As-induced disorders of amino acids and energy metabolism, which might therefore, play an important part in mechanisms of As neurotoxicity.Ting-Ting Mo, Hua Dai, and Hang Du contributed equally to the study.

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Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics

Cited by 22 publications

References 14 publications

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

Proteomic responses reveal the differential effects induced by cadmium in mussels Mytilus galloprovincialis at early life stages

Gas chromatography‐mass spectrometry based metabolomics profile of hippocampus and cerebellum in mice after chronic arsenic exposure

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