Impact of microparticles derived from erythrocytes on fibrinolysis

Levin, G. Ya.; Sukhareva, Ekaterina; Lavrentieva, Athina

doi:10.1007/s11239-015-1299-y

Cited by 23 publications

(17 citation statements)

References 31 publications

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“…As expected from in vitro studies demonstrating an increase in mechanical stability and retardation of fibrinolysis, similar effects were shown for thrombi in experimental cerebral ischemia . Alternatively, RBC‐derived MVs have a prominent fibrinolytic activity in vitro because of the presence of plasminogen on their surface . Moreover, a higher fraction of RBCs in cerebral thrombi has been shown to correlate with better responsiveness to intravenous thrombolysis .…”

Section: Interactions Of Rbcs With Cellular and Molecular Components mentioning

confidence: 99%

Red blood cells: the forgotten player in hemostasis and thrombosis

Weisel

Litvinov

2019

Journal of Thrombosis and Haemostasis

325

272

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Summary New evidence has stirred up a long‐standing but undeservedly forgotten interest in the role of erythrocytes, or red blood cells (RBCs), in blood clotting and its disorders. This review summarizes the most recent research that describes the involvement of RBCs in hemostasis and thrombosis. There are both quantitative and qualitative changes in RBCs that affect bleeding and thrombosis, as well as interactions of RBCs with cellular and molecular components of the hemostatic system. The changes in RBCs that affect hemostasis and thrombosis include RBC counts or hematocrit (modulating blood rheology through viscosity) and qualitative changes, such as deformability, aggregation, expression of adhesive proteins and phosphatidylserine, release of extracellular microvesicles, and hemolysis. The pathogenic mechanisms implicated in thrombotic and hemorrhagic risk include variable adherence of RBCs to the vessel wall, which depends on the functional state of RBCs and/or endothelium, modulation of platelet reactivity and platelet margination, alterations of fibrin structure and reduced susceptibility to fibrinolysis, modulation of nitric oxide availability, and the levels of von Willebrand factor and factor VIII in blood related to the ABO blood group system. RBCs are involved in platelet‐driven contraction of clots and thrombi that results in formation of a tightly packed array of polyhedral erythrocytes, or polyhedrocytes, which comprises a nearly impermeable barrier that is important for hemostasis and wound healing. The revisited notion of the importance of RBCs is largely based on clinical and experimental associations between RBCs and thrombosis or bleeding, implying that RBCs are a prospective therapeutic target in hemostatic and thrombotic disorders.

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Section: Interactions Of Rbcs With Cellular and Molecular Components mentioning

confidence: 99%

Red blood cells: the forgotten player in hemostasis and thrombosis

Weisel

Litvinov

2019

Journal of Thrombosis and Haemostasis

325

272

View full text Add to dashboard Cite

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“…It is known that the effect of α 2 -macroglobulin on plasmin is more pronounced than that on thrombin. Previously we revealed antiplasmin activity in eMV, which is probably associated with α 2 -macroglobulin [7]. Evidently, the presence of this protein in eMV can explain their slowly progressing ATA.…”

Section: Resultsmentioning

confidence: 74%

Antithrombin Activity of Erythrocyte Microvesicles

Levin

Sukhareva

2017

Bull Exp Biol Med

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Coagulation and optical (based on chromogenic substrate) methods were employed to examine antithrombin activity of erythrocytes and erythrocyte-derived microvesicles isolated days 7, 14, 21, and 28 on erythrocyte storage. The erythrocyte-derived microvesicles decelerated fibrin clot formation from fibrinogen in the presence of exogenous thrombin both with and without heparin. Microvesicles reduced optical density of chromogenic substrate. These data suggest that erythrocyte-derived microvesicles display a prominent antithrombin activity, which significantly increases during erythrocyte storage.

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“…Circulating lipids can lead to disturbances in the coagulation system and to cerebral and pulmonary embolism. Membrane lipids derived from cellular debris are known to be procoagulant and can also cause immunological reactions . Contamination of wound blood with fat particles frequently occurs in cardiac or orthopaedic surgery, where fat layers and circulating particles have been observed in wound blood .…”

Section: Discussionmentioning

confidence: 99%

A new approach for fat removal in a discontinuous autotransfusion device–concept and evaluation

et al. 2017

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Background Fat present during blood salvage in orthopaedic or cardiac surgery can pose a risk of fat embolism and should be eliminated before transfusion. Based on observations of central fat accumulation at the bottom of Latham bowls, a fat reduction program was developed using two volume displacements, where blood temporarily is removed and respun in the bowl to force the fat through the RBC sediment. Materials and MethodsPooled ABO-matched RBC and FFP were adjusted to a haematocrit of 10%, and human fat tissue added to a concentration of 1Á25 vol%. In six experiments, blood was processed with the new-generation cell salvage device CS Elite in a newly developed fat reduction program in bowls of three sizes. Volumetric quantification of fat was performed after centrifugation of blood samples in Pasteur pipettes. From volumes, haematocrits and the concentrations of fat, RBC recovery and fat elimination rates were calculated.Results Fat removal rates of 93Á2 -2Á8, 97Á0 -2Á1 and 99Á6 -0Á3% were observed with a 70-ml, 125-ml and 225-ml bowl, respectively, and even higher rates when removal rates were calculated one cycle. At the same time, high RBC recovery and plasma elimination rates were maintained, not significantly different to the default program mode.Conclusion Modifications in process parameters and sequence led to a fat reduction program that significantly improves fat removal with the Cell Saver Elite from 77Á4 -5Á1% in the default mode to an average of 98Á6 -1Á1%, yielding results equivalent to the continuous cell salvage system (C.A.T.S).

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Impact of microparticles derived from erythrocytes on fibrinolysis

Cited by 23 publications

References 31 publications

Red blood cells: the forgotten player in hemostasis and thrombosis

Red blood cells: the forgotten player in hemostasis and thrombosis

Antithrombin Activity of Erythrocyte Microvesicles

A new approach for fat removal in a discontinuous autotransfusion device–concept and evaluation

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