Impact of Mobilization Strategies on Peripheral Blood Stem Cell Collection Efficiency and Product Quality: A Retrospective Single-Center Study

Abstract

Autologous stem cell transplantation is routinely used in the management of several hematological diseases, solid tumors, and immune disorders. Peripheral blood stem cell (PBSC) collection performed by apheresis is the preferred source of stem cells. In this study, the potential impact of mobilization regimens on the performance of the Spectra Optia® continuous mononuclear cell collection system was evaluated. We performed a retrospective data analysis for patients undergoing autologous PBSC collection at the Medical University Vienna, Vienna General Hospital between September 2016 and June 2018. Collections were divided into two main groups according to the mobilization regimen received: without (210 collections) or with (99 collections) plerixafor. Assessed variables included product characteristics and collection efficiency (CE). Overall, product characteristics were similar between the groups. Median CD34+ CE2 was 50.1% versus 53.0%, and CE1 was 66.9% versus 69.9% following mobilization without and with plerixafor, respectively; the difference was not statistically significant. Simple linear regression showed a very weak positive correlation between the mobilization method and CE1 or CE2 (mobilization with plerixafor increased CE2 by 4.106%). In conclusion, the Spectra Optia® apheresis system led to high CE and a good quality of PBSC products when mobilization regimens with or without plerixafor were used.

The efficiency of a continuous versus an intermittent apheresis method for collection of hematopoietic progenitor stem cells: A systematic review

“…For example, male gender, younger donor age, elevated baseline white blood cell (WBC) count, and higher doses of granulocyte‐colony‐stimulating factor (G‐CSF) administration have been identified as predictors of high CD34+ yield 7 . Collection efficiency (CE %) can be defined as the number of cells that is collected from the total number of cells processed by the apheresis machine 8 …”

“…7 Collection efficiency (CE %) can be defined as the number of cells that is collected from the total number of cells processed by the apheresis machine. 8 Peripheral HPSC is undertaken via apheresis using centrifugation-based techniques. 9 This technique isolates mononuclear cells (MNCs), including CD34+ hematopoietic stem cells.…”