Impact of mucin, bile salts and cholesterol on the virulence of Vibrio anguillarum towards gnotobiotic sea bass (Dicentrarchus labrax) larvae

Li, Xuan; Bossier, Peter; Dierckens, Kristof; Laureau, Stanislas; Defoirdt, Tom

doi:10.1016/j.vetmic.2014.10.033

Cited by 17 publications

(11 citation statements)

References 32 publications

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“…) but virulence interactions with enterocytes are evident when the pathogen is in contact with mucin, bile salts and cholesterol as these intestinal factors trigger an increase in protease activity, flagellar motility, biofilm formation and exopolysaccharide production; respective expressions of genes emp A (metalloprotease), fle Q (flagellar transcriptional regulator), fla A (flagellin protein), che R (chemotaxis methyltransferase), wbf D (exopolysaccharide synthesis) and wza (exopolysaccharide export) are specifically enhanced under these conditions (Li et al . ).…”

Section: Pathology and Virulencementioning

confidence: 97%

“…It is believed that V. anguillarum might possess outer-membrane vesicles which transport virulence factors onto enterocytes for interaction and pathogenesis when colonized in the host intestine (Rekecki et al 2013). Vibrio anguillarum does not cause damage to saline-rinsed intestinal tissue ex vivo (Martinsen et al 2011) but virulence interactions with enterocytes are evident when the pathogen is in contact with mucin, bile salts and cholesterol as these intestinal factors trigger an increase in protease activity, flagellar motility, biofilm formation and exopolysaccharide production; respective expressions of genes empA (metalloprotease), fleQ (flagellar transcriptional regulator), flaA (flagellin protein), cheR (chemotaxis methyltransferase), wbfD (exopolysaccharide synthesis) and wza (exopolysaccharide export) are specifically enhanced under these conditions (Li et al 2015).…”

Section: Pathology and Virulencementioning

confidence: 99%

“…The virulence factors that are initiated following contact with such host-endogenous cues result in the growth and release of hypervirulent V. anguillarum cells which are easily transmitted by contact among fish populations that are densely contained in high aquaculture quantities therein (Li et al 2015). Blue oysters have been shown to accumulate and concentrate such virulent V. anguillarum cells which are released via faecal matter as a vector of enhanced pathogenicity in the marine environment (Pietrak et al 2012).…”

Section: Pathology and Virulencementioning

confidence: 99%

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A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Hickey

Lee

2017

Reviews in Aquaculture

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Vibrio (Listonella) anguillarum is a marine bacteria that is pathogenic to a number of aquatic organisms including several species which are important to the aquaculture industry. Organisms that are infected by V. anguillarum are diagnosed with vibriosis which can be lethal in a matter of days. Vibriosis can be particularly devastating to aquaculture businesses and measures of prevention or treatment are generally quite expensive. Efforts to understand and control V. anguillarum virulence have been of high‐priority among international aquatic research studies. The knowledge that has accumulated as a result of this collective research effort is reviewed in this article.

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Section: Pathology and Virulencementioning

confidence: 97%

Section: Pathology and Virulencementioning

confidence: 99%

Section: Pathology and Virulencementioning

confidence: 99%

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A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Hickey

Lee

2017

Reviews in Aquaculture

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“…This indicates that sensing of the host environment largely induces the T3SS. Previous research has shown that virulence gene expression can be induced in vibrios by several features of the host environment, including (but not limited to) low iron levels [ 25 ], low oxygen levels [ 26 ], catecholamines [ 27 ], mucin, cholesterol and bile salts [ 28 , 29 ] and low phosphate levels [ 30 ]. Further research will be needed to investigate which of these factors (if any) is responsible for the induction of the T3SS during infection.…”

Section: Resultsmentioning

confidence: 99%

Expression and Quorum Sensing Regulation of Type III Secretion System Genes of Vibrio harveyi during Infection of Gnotobiotic Brine Shrimp

et al. 2015

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Type III secretion systems enable pathogens to inject their virulence factors directly into the cytoplasm of the host cells. The type III secretion system of Vibrio harveyi, a major pathogen of aquatic organisms and a model species in quorum sensing studies, is repressed by the quorum sensing master regulator LuxR. In this study, we found that during infection of gnotobiotic brine shrimp larvae, the expression levels of three type III secretion operons in V. harveyi increased within the first 12h after challenge and decreased again thereafter. The in vivo expression levels were highest in a mutant with a quorum sensing system that is locked in low cell density configuration (minimal LuxR levels) and lowest in a mutant with a quorum sensing system that is locked in the high cell density configuration (maximal LuxR levels), which is consistent with repression of type III secretion by LuxR. Remarkably, in vivo expression levels of the type III secretion system genes were much (> 1000 fold) higher than the in vitro expression levels, indicating that (currently unknown) host factors significantly induce the type III secretion system. Given the fact that type III secretion is energy-consuming, repression by the quorum sensing master regulators might be a mechanism to save energy under conditions where it does not provide an advantage to the cells.

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“…Mucin, a natural barrier widely found in animals, always plays critical role in antiviral and antibacterial activities [27][28][29][30]. OVM is a mucin protein in egg.…”

Section: Discussionmentioning

confidence: 99%

Sialic acid involves in the interaction between ovomucin and hemagglutinin and influences the antiviral activity of ovomucin

Shan

Wang

et al. 2018

International Journal of Biological Macromolecules

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Ovomucin (OVM) plays an important role in inhibiting infection of various pathogens. However, this bioactivity mechanism is not much known. Here, the role of sialic acid in OVM anti-virus activity has been studied by ELISA with lectin or ligand. Structural changes of OVM after removing sialic acid were analyzed by circular dichroism and fluorescence spectroscopy. OVM could be binding to the hemagglutinin (HA) of avian influenza viruses HN and HN, this binding was specific and required the involvement of sialic acid. When sialic acid was removed, the binding was significantly reduced 71.5% and 64.35%, respectively. Therefore, sialic acid was proved as a recognition site which avian influenza virus bound to. Meanwhile, the endogenous fluorescence and surface hydrophobicity of OVM removing sialic acid were increased and the secondary structure tended to shift to random coil. This indicated that OVM molecules were in an unfolded state and spatial conformation disorder raising weakly. Remarkably, free sialic acid strongly promoted OVM binding to HA and thereby enhanced the interaction. It may contribute to the inhibition of host cell infection, agglutinate viruses. This study can be extended to the deepening of passive immunization field.

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Impact of mucin, bile salts and cholesterol on the virulence of Vibrio anguillarum towards gnotobiotic sea bass (Dicentrarchus labrax) larvae

Cited by 17 publications

References 32 publications

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Expression and Quorum Sensing Regulation of Type III Secretion System Genes of Vibrio harveyi during Infection of Gnotobiotic Brine Shrimp

Sialic acid involves in the interaction between ovomucin and hemagglutinin and influences the antiviral activity of ovomucin

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