Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Meziu, Enkeleda; Shehu, Kristela; Koch, Marcus; Schneider, Marc; Kraegeloh, Annette

doi:10.1016/j.ijpx.2023.100212

Cited by 3 publications

(4 citation statements)

References 74 publications

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“…The cryo-SEM preparation process was examined for its potential impact on mucus structure. 61 , 63 , 67 The pore size distribution of the examined BSM was found to be mainly between 10 and 80 nm ( Figure 2 C). This is in the expected size range based on nanoparticle diffusion experiments in native mucus and substantially smaller than the reported pore sizes of artificial pores (micrometer range), which can be caused by the cryogenic preparation process.…”

Section: Resultsmentioning

confidence: 98%

“…Overall, the network structure, including the pores and pore size distribution in the mucus appeared heterogeneous This goes in line with the pore size results from (cryo-) SEM studies from i.e., human airway mucus − or porcine and canine gastric mucus collections ,, Thus, BSM shows an internal network comparable to other mucus systems. The cryo-SEM preparation process was examined for its potential impact on mucus structure. ,, The pore size distribution of the examined BSM was found to be mainly between 10 and 80 nm (Figure C). This is in the expected size range based on nanoparticle diffusion experiments in native mucus and substantially smaller than the reported pore sizes of artificial pores (micrometer range), which can be caused by the cryogenic preparation process .…”

Section: Resultsmentioning

confidence: 99%

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Comprehensive Characterization of the Viscoelastic Properties of Bovine Submaxillary Mucin (BSM) Hydrogels and the Effect of Additives

Rulff,

Schmidt,

Wei

et al. 2024

Biomacromolecules

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This study presents a comprehensive characterization of the viscoelastic and structural properties of bovine submaxillary mucin (BSM), which is widely used as a commercial source to conduct mucus-related research. We conducted concentration studies of BSM and examined the effects of various additives, NaCl, CaCl 2 , MgCl 2 , lysozyme, and DNA, on its rheological behavior. A notable connection between BSM concentration and viscoelastic properties was observed, particularly under varying ionic conditions. The rheological spectra could be well described by a fractional Kelvin−Voigt model with a minimum of model parameters. A detailed proteomics analysis provided insight into the protein, especially mucin composition within BSM, showing MUC19 as the main component. Cryo-scanning electron microscopy enabled the visualization of the porous BSM network structure. These investigations give us a more profound comprehension of the BSM properties, especially those pertaining to viscoelasticity, and how they are influenced by concentration and environmental conditions, aspects relevant to the field of mucus research.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Comprehensive Characterization of the Viscoelastic Properties of Bovine Submaxillary Mucin (BSM) Hydrogels and the Effect of Additives

Rulff,

Schmidt,

Wei

et al. 2024

Biomacromolecules

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show abstract

“…human airway mucus [52][53][54] or porcine and canine gastric mucus collections 45,55,56 Thus, BSM shows an internal network comparable to other mucus systems. The cryo-SEM preparation process was examined for its potential impact on mucus structure 51,53,57 . The cryo-SEM images of the BSM displayed structures that were similar to those of mucus previously analyzed from various sources.…”

Section: Cryo-sem Images Visualize Bsm Networkmentioning

confidence: 99%

Comprehensive characterization of the viscoelastic properties of Bovine Submaxillary Mucin (BSM) hydrogels and the effect of additives

Rulff,

Schmidt,

Wei

et al. 2024

Preprint

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This study presents a comprehensive characterization of the viscoelastic and structural properties of Bovine Submaxillary Mucin (BSM), a good biomaterial model system for mucins and mucus in general. We conducted concentration studies of BSM and examined the effects of various additives - sodium chloride, calcium chloride, lysozyme, and DNA - on its rheological behavior. Through oscillatory and shear flow macrorheological experiments, we established a detailed correlation between the concentration of BSM and its viscoelastic properties, uncovering changes in the rheological moduli with the addition of sodium and calcium chloride, particularly at higher concentrations. This highlights the mucins responsiveness to ionic strength and composition variations. The presence of lysozyme and DNA further influenced the rheological characteristics of BSM, adding a new dimension to our understanding of mucin behavior. The rheological spectra could be well-described by a Fractional Kelvin-Voigt Model (FKVM), which allows to describe the complete behavior with a minimum of model parameters. A detailed proteomics analysis provided insight into the molecular interactions within BSM, enhancing our understanding of its structural intricacies. Complementing this, cryo-scanning electron microscopy (cryo-SEM) was employed to visualize the microstructural alterations and network properties in relation to the rheological data. By elucidating the complex interplay between mucin concentration, environmental conditions, and viscoelastic properties, this research significantly contributes to the field of mucus research and lays an important basis for future studies and advanced applications of numerous mucin-based biomaterials.

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“…The free sulfhydryl of the inhaled compounds could be oxidized and form disulfide bonds with mucins, which lock them in and reduce their penetration. [ 37 ] In addition, the carboxyl groups on the mucin proteoglycans exhibited negative charges, which readily combine with the vehicles with positive charges, especially nanocarriers for gene delivery, and trap them via electrostatic interaction. Several researchers have confirmed the particles with negative or neutral surface charges exhibited a rapid mucus penetrating rate compared to those with positive surface charges.…”

Section: Factors Influencing the Pulmonary Bioavailability Of The Inh...mentioning

confidence: 99%

Recent Progress in Nucleic Acid Pulmonary Delivery toward Overcoming Physiological Barriers and Improving Transfection Efficiency

Wang,

Bu,

Dai

et al. 2024

Advanced Science

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Pulmonary delivery of therapeutic agents has been considered the desirable administration route for local lung disease treatment. As the latest generation of therapeutic agents, nucleic acid has been gradually developed as gene therapy for local diseases such as asthma, chronic obstructive pulmonary diseases, and lung fibrosis. The features of nucleic acid, specific physiological structure, and pathophysiological barriers of the respiratory tract have strongly affected the delivery efficiency and pulmonary bioavailability of nucleic acid, directly related to the treatment outcomes. The development of pharmaceutics and material science provides the potential for highly effective pulmonary medicine delivery. In this review, the key factors and barriers are first introduced that affect the pulmonary delivery and bioavailability of nucleic acids. The advanced inhaled materials for nucleic acid delivery are further summarized. The recent progress of platform designs for improving the pulmonary delivery efficiency of nucleic acids and their therapeutic outcomes have been systematically analyzed, with the application and the perspectives of advanced vectors for pulmonary gene delivery.

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Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Cited by 3 publications

References 74 publications

Comprehensive Characterization of the Viscoelastic Properties of Bovine Submaxillary Mucin (BSM) Hydrogels and the Effect of Additives

Comprehensive Characterization of the Viscoelastic Properties of Bovine Submaxillary Mucin (BSM) Hydrogels and the Effect of Additives

Comprehensive characterization of the viscoelastic properties of Bovine Submaxillary Mucin (BSM) hydrogels and the effect of additives

Recent Progress in Nucleic Acid Pulmonary Delivery toward Overcoming Physiological Barriers and Improving Transfection Efficiency

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