Introduction: Logistical and financial barriers have traditionally prevented formal American Heart Association (AHA) advanced cardiovascular life support (ACLS) training from being taught in the core Doctor of Pharmacy (PharmD) curriculum. The objective of this study was to determine the benefit of ACLS training on knowledge and confidence of PharmD students. Methods: Two modes of ACLS instruction were administered: (1) didactic lecture followed by skills assessment using low-fidelity simulation taught to all 3 rd year PharmD students (n=133) and (2) formal AHA ACLS training offered to a subset of students (n=24). Three assessments were administered: a pre-and post-test of all students assessing knowledge and confidence of ACLS and an additional post-test after completing formal AHA ACLS training. Changes in knowledge and confidence were compared with the Chi-squared and Mann Whitney U tests, as appropriate. Results: Formal ACLS certification resulted in increased knowledge of ACLS (75.6% vs 85%, p=0.045). Student confidence also improved after ACLS certification, with identification as "expert" increasing from 1 to 3 areas (p=0.004) and self-ratings of novice, advanced beginner, and competent all decreasing, indicating a shift towards higher confidence levels. Conclusion: Formal AHA training with high-fidelity mannequins improved pharmacy student knowledge and confidence of ACLS fundamentals.