Impact of natural curcumin on the progression of experimental periodontitis in diabetic rats

Abstract

Objective To evaluate the role of natural curcumin (CURC) on experimental periodontitis (EP) in animals with diabetes mellitus (DM). Material and Methods One hundred rats were assigned to DM + placebo (PLA); DM + CURC; DM + insulin (INS); DM + CURC + INS; and Non‐DM. Diabetes was induced by streptozotocin. After 3 days, they were initiated CURC and PLAC solutions and insulin administrations, daily for 30 days. This included a period of 19 days prior to EP induction (ligature at the first mandibular and the second maxillary molar) and then additional 11 days. Specimens from the mandible were processed for morphometric examination of bone level. Gingival tissues from mandibular molars were collected for quantification of IL‐1β, IL‐4, IL‐6, IL‐17, IFN‐γ, and TNF‐α using a Luminex/MAGpix assay. Gingivae from maxillary molars were subjected to RT‐PCR for assessment of Runx2, RANKL, OPG, SIRT, Dkk1, and Sost levels. Results Lower linear bone loss was detected in ligated molars of DM + CURC + INS vs DM + PLAC and DM + INS groups (P < 0.05). In ligated sites from DM rats treated with CURC + INS, IL‐6, IL‐1β, INF‐γ, and TNF‐α levels were the lowest in comparison with PLAC and/or INS and CURC as monotherapies (P < 0.05). CURC, independently of INS, increased Runx2 and SIRT when compared to DM + PLAC (P < 0.05) in ligated sites, whereas only CURC + INS reduced the RANKL/OPG ratio when compared to DM + PLAC (P < 0.05). Conclusion Natural CURC, when associated with INS, reduces the DM‐induced loss of supporting alveolar bone and promotes favorable modulation on osteo‐immune‐inflammatory mediators.

Resveratrol and insulin association reduced alveolar bone loss and produced an antioxidant effect in diabetic rats

“…A stock solution of RSV ¶ molecular weight: 228.2) was prepared in Tween-80 # and diluted further in water to obtain a working concentration to provide safe and convenient ingestion volume (1 mL) to dose the animals at a level of 10 mg/kg of RSV. [16][17][18][19][20] The PLAC was prepared with the same volume of Tween-80 and water used in the preparation of RSV. offered daily via gavage.…”

“…Insulin was administered daily by subcutaneous injection of neutral protamine Hagedorn ‖ diluted in 0.9% NaCl -5.5 to 3.5 mL at 6:00 pm and at 6 am. 20 All treatments (RSV, PLAC, and INS) were performed for 30 days. A period of 19 days before EP induction and then for another 11 days after that also is included.…”

“…5,13,14 The control of periodontal breakdown in diabetic patients may be a challenge because of the altered release of inflammatory factors 12,15 and to the oxidative stress status. 13 In this context, alternative therapies have been investigated that focus on the concept of host-modulation therapy to alter host response to microbial infection, and more specifically to downregulate oxidative stress with the aim of improving host modulation, specially using natural products [16][17][18][19][20] as well as the production of matrix metalloproteinases. 21,22 For the purposes of this investigation we shall focus on oxidative stress and approaches that could be used to downregulate this damaging pathobiological phenomenon.…”

The Impact of Curcumin on Bone Osteogenic Promotion of MC3T3 Cells under High Glucose Conditions and Enhanced Bone Formation in Diabetic Mice

“…Previous studies have suggested a positive effect of curcumin on bone formation in diabetes and diabetes-related periodontitis [22]. However, few studies have investigated the effectiveness of natural curcumin on alveolar bone formation in diabetic osteoporosis, and there is also a lack of investigations into cortical bone and cancellous bone.…”

“…More importantly, it is reported that curcumin could suppress osteoclast activities by inhibiting the expression of transcription factor AP-1 [21]. Moreover, curcumin, in combination with insulin, inhibits alveolar bone loss of experimental periodontitis in diabetic rats [22].…”