Impact of non-surgical periodontal treatment on salivary expression of cytokines related to bone metabolism

Beiler, Tatiane F C S B; Mello‐Neto, João Martins de; Alves, Juliana Cardoso; Hamlet, Stephen; Ipe, Deepak S.; Figueredo, Carlos Marcelo

doi:10.1007/s10266-020-00502-2

Cited by 10 publications

(7 citation statements)

References 20 publications

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“…It is generally agreed that non-surgical periodontal treatment can result in downregulation of pro-inflammatory cytokines associated with bone connective tissue and bone metabolism. The majority of studies demonstrated significant reduction of salivary concentrations of Il-1β, IL-6, IL-8, TNF-α and GM-CSF, as well as increases in IL-4 and IL-10 [33][34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Omega-3 Polyunsaturated Fatty Acids EPA and DHA as an Adjunct to Non-Surgical Treatment of Periodontitis: A Randomized Clinical Trial

et al. 2020

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Periodontitis is a chronic multifactorial inflammatory disease that leads to the loss of supportive tissues around the teeth with gradual deterioration of masticatory function and esthetics, resulting eventually in the decrease of the life quality. Host immune response triggered by bacterial biofilm is responsible for the chronic periodontal inflammation and ongoing tissue loss. Omega-3 polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have anti-inflammatory properties, thus may be used for the treatment of chronic inflammatory diseases. In this study, we aimed to evaluate the effect of dietary supplementation with omega-3 PUFA in the patients with stage III and IV periodontitis. Thirty otherwise healthy patients were treated with scaling and root planning (SRP). In the test group (n = 16), patients were additionally supplemented with 2.6 g of EPA and 1.8 g of DHA. In the control group (n = 14), patients received only SRP. Periodontal examination was performed at baseline and three months following initial therapy. Salivary samples were taken twice at baseline and at the end of the experiment. We found that there was a statistically significant reduction in the bleeding on probing (BOP) and improvement of clinical attachment loss (CAL) at three months in the test group compared to the control group. Moreover, a statistically significant higher percentage of closed pockets (probing depth ≤ 4 mm without BOP) was achieved in the test group vs. control group after three months of treatment. Accordingly, the levels of pro-inflammatory cytokines/chemokines interleukin (IL)-8 and IL-17 were markedly lower, while the level of anti-inflammatory IL-10 was significantly higher in the salivary samples of the patients supplemented with omega-3 PUFA at three months in comparison to the patients treated with SRP alone. Our findings demonstrate that dietary intervention with high-dose of omega-3 PUFA during non-surgical therapy may have potential benefits in the management of periodontitis.

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Section: Discussionmentioning

confidence: 99%

Omega-3 Polyunsaturated Fatty Acids EPA and DHA as an Adjunct to Non-Surgical Treatment of Periodontitis: A Randomized Clinical Trial

et al. 2020

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“…Treatment with anti-Sclerostin antibody enhances alveolar bone mass, leads to the formation of cellular cementum and increases alveolar bone deposition [ 11 , 35 ]. Data from clinical studies have shown that SOST is upregulated in periodontal tissues, saliva and GCF of chronic periodontitis patients when compared to healthy sites [ 36 , 37 , 38 , 39 ]. In the present study, although there was a trend towards higher Sclerostin amounts in the gingivitis sites ( p = 0.09), no significant differences were observed between periodontitis, gingivitis and healthy sites in periodontitis patients ( p > 0.05).…”

Section: Discussionmentioning

confidence: 99%

WNT-5a and SOST Levels in Gingival Crevicular Fluid Depend on the Inflammatory and Osteoclastogenic Activities of Periodontal Tissues

et al. 2021

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Background and Objectives: Wnt signaling leads to stimulation of osteoblasts and it reduces osteoclastogenesis and bone resorption via the regulation of the osteprotegrin and receptor activator of nuclear factor kappa-Β ligan (RANKL). Wnt signaling pathways are regulated by their physiological antagonists such as sclerostin (SOST) as well as WNT-5a. The aim of this study was to determine the total amount of Sclerostin and WNT-5a in the gingival crevicular fluid (GCF) in sites with a continuum from a healthy to diseased periodontium. Materials and Methods: In this cross-sectional study, a total of 20 patients with generalized periodontitis, 10 subjects with gingivitis as well as 14 individuals with a healthy periodontium were recruited upon clinical and radiographic periodontal examination. In patients diagnosed with periodontitis, GCF samples were collected from periodontitis, gingivitis and healthy sites, while gingivitis patients provided samples from gingivitis and healthy sites. In healthy patients, only healthy sites were sampled. Protein total amount of SOST and WNT-5a were quantified by sandwich enzyme-linked immunosorbent assay (ELISA). Results: A total of 108 GCF samples were collected from a total of 44 individuals. When all periodontitis (n = 51), gingivitis (n = 12) and healthy (n = 45) sites were analyzed regardless of the patient diagnosis, periodontitis sites demonstrated significantly elevated WNT-5a total amounts (p = 0.03) when compared to gingivitis sites. Gingivitis sites demonstrated a trend of more total SOST (p = 0.09) when compared to periodontitis and healthy sites. Within each patient diagnostic category, sites showed similar SOST and WNT-5a total amounts (p > 0.05). Conclusions: WNT-5a levels in GCF depend on the stage of periodontitis sites. SOST trended higher in the GCF of gingivitis sites but similar in chronic periodontitis and healthy sites. WNT-5a and SOST play a crucial role in periodontal tissue remodeling and depend on the inflammatory and osteoclastogenic activities.

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“…37 Patients with chronic periodontitis had 1.6 times higher levels of sclerostin expression than at baseline, but Beiler et al found no significant difference between salivary sclerostin levels prior to and following NSPT. 38…”

Section: Sclerostin Distribution Within Oral Tissuesmentioning

confidence: 99%

Sclerostin - The silent bone breaker

ROSHNİ,

SHETTY,

GİRİDHAR KAMATH

2023

Cumhuriyet Dental Journal

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A disparity between host defense and periodontopathogens leads to periodontitis, which is an inflammatory disease of the periodontium of high prevalence. The dysregulated host immune response brought on by the disease’s ongoing progression may result in tissue and bone destruction, which ultimately leads to tooth loss. Interpretation of bone metabolism has enhanced as a result of the identification of sclerostin and its function as a bone mass regulator. Primarily, osteocytes express sclerostin, an SOST gene known to inhibit formation of bone. The canonical Wnt pathway involved in bone homeostasis, is significantly suppressed by Sclerostin. It is thought to result in resorption of bone by altering the ratio of OPG and RANKL. Characteristics, mode of action and significance of sclerostin in periodontal diseases are discussed in this review.

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Impact of non-surgical periodontal treatment on salivary expression of cytokines related to bone metabolism

Cited by 10 publications

References 20 publications

Omega-3 Polyunsaturated Fatty Acids EPA and DHA as an Adjunct to Non-Surgical Treatment of Periodontitis: A Randomized Clinical Trial

Omega-3 Polyunsaturated Fatty Acids EPA and DHA as an Adjunct to Non-Surgical Treatment of Periodontitis: A Randomized Clinical Trial

WNT-5a and SOST Levels in Gingival Crevicular Fluid Depend on the Inflammatory and Osteoclastogenic Activities of Periodontal Tissues

Sclerostin - The silent bone breaker

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