Impact of Nosema ceranae and Nosema apis on individual worker bees of the two host species (Apis cerana and Apis mellifera) and regulation of host immune response

Sinpoo, Chainarong; Paxton, Robert J.; Disayathanoowat, Terd; Krongdang, Sasiprapa

doi:10.1016/j.jinsphys.2017.12.010

Cited by 62 publications

(73 citation statements)

References 70 publications

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“…However, in places where both parasites exist, such as in Canada, USA and Europe, infections by N. ceranae have become more common than infections by N. apis [6][7][8]. N. ceranae causes suppression of the immune response in honey bees by downregulating the expression of antimicrobial peptide (AMP) genes [9], although another study showed that N. apis and N. ceranae can also up-regulate AMP genes of honey bees [10]. In addition, N. ceranae infections cause increased energetic stress on the bee, leading to degeneration of epithelial cells and significantly shortening its life span [3,11].…”

Section: Introductionmentioning

confidence: 99%

Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds

Borges

Guzmán-Novoa

2020

PLoS ONE

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Nosema ceranae is a microsporidian parasite that causes nosemosis in the honey bee (Apis mellifera). As alternatives to the antibiotic fumagillin, ten nutraceuticals (oregano oil, thymol, carvacrol, trans-cinnmaldehyde, tetrahydrocurcumin, sulforaphane, naringenin, embelin, allyl sulfide, hydroxytyrosol) and two immuno-stimulatory compounds (chitosan, poly I:C) were examined for controlling N. ceranae infections. Caged bees were inoculated with N. ceranae spores, and treatments were administered in sugar syrup. Only two compounds did not significantly reduce N. ceranae spore counts compared to the infected positive control, but the most effective were sulforaphane from cruciferous vegetables, carvacrol from oregano oil, and naringenin from citrus fruit. When tested at several concentrations, the highest sulforaphane concentration reduced spore counts by 100%, but also caused 100% bee mortality. For carvacrol, the maximum reduction in spore counts was 57% with an intermediate concentration and the maximum bee mortality was 23% with the highest concentration. For naringenin, the maximum reduction in spore counts was 64% with the highest concentration, and the maximum bee mortality was only 15% with an intermediate concentration. In the longevity experiment, naringenin-fed bees lived as long as Nosema-free control bees, both of which lived significantly longer than infected positive control bees. While its antimicrobial properties may be promising, reducing sulforaphane toxicity to bees is necessary before it can be considered as a candidate for controlling N. ceranae. Although further work on formulation is needed with naringenin, its effect on extending longevity in infected bees may give it an additional value as a potential additive for bee feed in honey bee colonies.

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Section: Introductionmentioning

confidence: 99%

Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds

Borges

Guzmán-Novoa

2020

PLoS ONE

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“…Nosema infects the epithelial cells of the midgut of honey bee adults (Webster 1993) and larvae (Eiri et al 2015). Nosema ceranae is native to Eastern honey bee colonies (Apis cerana) but has rapidly spread since the 1990s to A. mellifera colonies around the world and has evidently largely displaced N. apis (Klee et al 2007;Papini et al 2017;Sinpoo et al 2018). Bees can be treated with the antibiotic fumagillin, which inhibits the enzyme methionine aminopeptidase-2 and thereby disrupts spore protein maturation (Sin et al 1997;Huang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Nicotine does not reduce Nosema ceranae infection in honey bees

et al. 2020

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Your article is protected by copyright and all rights are held exclusively by International Union for the Study of Social Insects (IUSSI). This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

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“…Vg was active even against Paenibacillus larvae, a gram-positive bacterium infesting young honeybee larvae and causing a disease called American foulbrood, probably the deadliest bee brood disease worldwide . In addition, it has been reported that infection of honey bee larvae by the spores of the microsporidium Nosema ceranae significantly upregulated Vg expression in workers (BenVau and Nieh, 2017;Sinpoo et al, 2018), and that bee Vg interacted with the cell wall of the entomopathogenic fungus Beauveria bassiana, eliciting membrane disruption and permeabilization. Furthermore, Vg appears to induce transgenerational immune priming in bee queens, enhancing immunity in their offspring by transporting pathogenassociated pattern molecules, which are attached to Vg, into the eggs within queen ovaries (Sadd et al, 2005;Salmela et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Changes in vitellogenin expression caused by nematodal and fungal infections in insects

Kodrı́k

Ibrahim

Gautam

et al. 2019

Journal of Experimental Biology

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This study examined the expression and role of vitellogenin (Vg) in the body of the firebug Pyrrhocoris apterus (Heteroptera, Insecta) during infection elicited by two entomopathogenic organisms, the nematode Steinernema carpocapsae and the fungus Isaria fumosorosea. Infection by S. carpocapsae significantly upregulated Vg mRNA expression in the male body. The corresponding increase in Vg protein expression was also confirmed by electrophoretic and immunoblotting analyses. Remarkably, in females, the opposite tendency was noted. Nematodal infection significantly reduced both Vg mRNA and Vg protein expression levels in fat body and hemolymph, respectively. We speculate that infection of reproductive females reduces Vg expression to a level that is still sufficient for defense, but is insufficient for reproduction. This circumstance reduces energy expenditure and helps the individual to cope with the infection. Importantly, purified Vg significantly inhibited growth of Xenorhabdus spp., an entomotoxic bacteria isolated from S. carpocapsae. However, the effect of Vg against I. fumosorosea was not so obvious. The fungus significantly stimulated Vg gene expression in males; however, a similar increase was not recapitulated at the protein level. Nevertheless, in females, both mRNA and protein Vg levels were significantly reduced after the fungal infection. The obtained data demonstrate that Vg is probably an important defense protein, possibly with a specific activity. This considerably expands the known spectrum of Vg functions, as its primary role was thought to be limited to regulating egg development in the female body.

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Impact of Nosema ceranae and Nosema apis on individual worker bees of the two host species (Apis cerana and Apis mellifera) and regulation of host immune response

Cited by 62 publications

References 70 publications

Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds

Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds

Nicotine does not reduce Nosema ceranae infection in honey bees

Changes in vitellogenin expression caused by nematodal and fungal infections in insects

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