Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)

Hernroth, Bodil; Baden, Susanne; Tassidis, Helena; Hörnaeus, Katarina; Guillemant, Julie; Lind, Sara Bergström; Bergquist, Jonas

doi:10.1016/j.fsi.2016.04.007

Cited by 28 publications

(10 citation statements)

References 64 publications

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“…Value of the data The data further validate the protein expression changes presented in Hernroth et al [1] . The data can be used to validate protein identification in Mytilus edulis from other studies.…”

supporting

confidence: 80%

“… Here, we provide the dataset associated with our research article on the potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis , “Impact of ocean acidification on antimicrobial activity in gills of the blue mussel ( Mytilus edulis )” [1] . Blue mussels were stimulated with lipopolysaccharides and samples were collected at different time points post injection.…”

mentioning

confidence: 99%

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Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)

Hörnaeus

Guillemant

et al. 2016

Data in Brief

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Here, we provide the dataset associated with our research article on the potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, “Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)” [1]. Blue mussels were stimulated with lipopolysaccharides and samples were collected at different time points post injection. Protein extracts were prepared from the gills, digested using trypsin and a full in-depth proteome investigation was performed using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Protein identification and quantification was performed using the MaxQuant 1.5.1.2 software, “MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification” [2].

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supporting

confidence: 80%

mentioning

confidence: 99%

Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)

Hörnaeus

Guillemant

et al. 2016

Data in Brief

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“…A growing body of evidence has suggested that OA could disrupt the energy balance of mollusks by impairing key processes associated with high energy expenditure, such as immunity, development, acid–base homeostasis maintenance, and antioxidant processes ( Lannig et al, 2010 ). A disturbance in energy metabolism by OA could sensitize bivalves to other environmental perturbations such as pathogen infection ( Ivanina and Sokolova, 2015 ; Hernroth et al, 2016 ). In this study, the suppressed expression of two genes (SDH and SCS) in OA-treated oysters suggested only a mild inhibition effect of OA on aerobic energy metabolism.…”

Section: Discussionmentioning

confidence: 99%

Seawater Acidification Reduced the Resistance of Crassostrea gigas to Vibrio splendidus Challenge: An Energy Metabolism Perspective

et al. 2018

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Negative physiological impacts induced by exposure to acidified seawater might sensitize marine organisms to future environmental stressors, such as disease outbreak. The goal of this study was to evaluate if ocean acidification (OA) could reduce the resistance capability of the Pacific oyster (Crassostrea gigas) to Vibrio splendidus challenge from an energy metabolism perspective. In this study, the Pacific oyster was exposed to OA (pH 7.6) for 28 days and then challenged by V. splendidus for another 72 h. Antioxidative responses, lipid peroxidation, metabolic (energy sensors, aerobic metabolism, and anaerobic metabolism) gene expression, glycolytic enzyme activity, and the content of energy reserves (glycogen and protein) were investigated to evaluate the environmental risk of pathogen infection under the condition of OA. Our results demonstrated that following the exposure to seawater acidification, oysters exhibited an energy modulation with slight inhibition of aerobic energy metabolism, stimulation of anaerobic metabolism, and increased glycolytic enzyme activity. However, the energy modulation ability and antioxidative regulation of oysters exposed to seawater acidification may be overwhelmed by a subsequent pathogen challenge, resulting in increased oxidative damage, decreased aerobic metabolism, stimulated anaerobic metabolism, and decreased energy reserves. Overall, although anaerobic metabolism was initiated to partially compensate for inhibited aerobic energy metabolism, increased oxidative damage combined with depleted energy reserves suggested that oysters were in an unsustainable bioenergetic state and were thereby incapable of supporting long-term population viability under conditions of seawater acidification and a pathogen challenge from V. splendidus.

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“…Many kinds of nutritional and pharmaceutical benefits of Mytilus edulis have been reported, such as nourishing the liver and kidneys, adjusting blood pressure, curing night sweats, dizziness and impotence, and so on [ 5 ]. More and more bioactive peptides from the M. edulis whole body rather than different tissues, such as antimicrobial peptides [ 6 , 7 ], anti-inflammatory peptides [ 5 ], antioxidant peptides [ 8 , 9 ] and anticoagulant peptides, have been reported in recent years. Meanwhile, the bioactive peptides from different tissues of M. edulis are identifiable [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…The antithrombotic activity of M. edulis hydrolysate has been reported in several studies [ 6 ]. However, anticoagulant peptides were rarely isolated from M. edulis , and interactions between the peptide and thrombin have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins

Qiao

Chen

et al. 2018

IJMS

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Mytilus edulis is a typical marine bivalve mollusk. Many kinds of bioactive components with nutritional and pharmaceutical activities in Mytilus edulis were reported. In this study, eight different parts of Mytilus edulis tissues, i.e., the foot, byssus, pedal retractor muscle, mantle, gill, adductor muscle, viscera, and other parts, were separated and the proteins from these tissues were prepared. A total of 277 unique peptides from the hydrolysates of different proteins were identified by UPLC-Q-TOF-MS/MS, and the molecular weight distribution of the peptides in different tissues was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The bioactivity of the peptides was predicted through the Peptide Ranker database and molecular docking. Moreover, the peptides from the adductor muscle were chosen to do the active validation of anticoagulant activity. The active mechanism of three peptides from the adductor muscle, VQQELEDAEERADSAEGSLQK, RMEADIAAMQSDLDDALNGQR, and AAFLLGVNSNDLLK, were analyzed by Discovery Studio 2017, which also explained the anticoagulant activity of the hydrolysates of proteins from adductor muscle. This study optimized a screening and identification method of bioactive peptides from enzymatic hydrolysates of different tissues in Mytilus edulis.

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Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)

Cited by 28 publications

References 64 publications

Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)

Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)

Seawater Acidification Reduced the Resistance of Crassostrea gigas to Vibrio splendidus Challenge: An Energy Metabolism Perspective

Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins

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