Impact of operating condition on the denitrifying bacterial community structure in a 3DBER-SAD reactor

Hao, Ruixia; Meng, Chengcheng; Li, Jianbing

doi:10.1007/s10295-016-1853-4

Cited by 12 publications

(15 citation statements)

References 31 publications

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“…A novel coupled system using constructed wetland and biofilm‐electrode reactor (CW–BER) was developed to treat low C/N wastewater, and the highest removal extent of NO 3 − ‐N was 63.0% under optimum operating conditions (C/N 0.75–1, current 15 mA, HRT 12 h and pH 7.5) . The mean total nitrogen removal was up to 85% in a 3DBER‐SAD (an integrated system of three‐dimensional electrode reactor and sulfur autotrophic denitrification) with 60 mA current . Hao et al .…”

Section: Resultsmentioning

confidence: 99%

Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor

Zhai

Pavlostathis

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND: A biofilm-electrode reactor (BER) was developed on the basis of hydrogenotrophic denitrification. The BER can be strengthened by adding organic matter, and the nitrate removal will be enhanced by a combination of heterotrophic and autotrophic denitrification. Denitrification in a biological system can be affected by operating parameters, and the microbial community and functional genes can be changed at the same time. In this study, the effect of hydraulic retention time (HRT) and current on the denitrification of a low chemical oxygen demand to nitrogen ratio (COD/N), saline wastewater in a continuous-flow BER was evaluated and compared to a conventional biofilm reactor (BR). RESULTS:The highest extent of nitrate removal was obtained at the optimum HRT (8 h) and current (10 mA). Low levels of ammonia (0.5-4 mg L −1 ), produced through dissimilatory nitrate reduction to ammonia (DNRA), were observed. Quantitative real-time polymerase chain reaction analysis revealed that the proportion of nirS-type denitrifiers (3.75-8.30%) exceeded nirK-type denitrifiers (0.24-0.90%) in both BR and BER. The genera of T78, Paracoccus and Azoarcus were identified as the dominant denitrifying bacteria in the BER. The cooperative activity of nitrate, nitrite and nitrous oxide reductase is necessary to reduce nitrate to dinitrogen in the denitrification process. The highest ratio of nosZ/nir genes in the bacterial community was 0.25 when the applied current was 10 mA. CONCLUSION: Compared to other denitrification reactors, the BER is more effective to treat low-COD/N, saline wastewater with short HRT and low current. RESULTS AND DISCUSSION Effect of HRT on denitrification performanceThe denitrification performance of BR and BER was investigated at five HRTs, decreasing from 16 to 12, 8, 4 and 2 h. The variation J Chem Technol Biotechnol 2019; 94: 933-941

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Section: Resultsmentioning

confidence: 99%

Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor

Zhai

Pavlostathis

et al. 2018

J of Chemical Tech & Biotech

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“…Thauera is a dominant Gram-negative organotrophic bacterium belonging to β-proteobacteria which has been identified in wastewater treatment systems [35], in an integrated system of three-dimensional biofilm-electrode reactor and sulfur autotrophic denitrification (3DBER-SAD) under mixotrophic conditions [27]. Thauera has also been identified in sequential batch reactors where the heterotrophic and autotrophic denitrifying process was conducted [23] and in several denitrifying bioreactors under autotrophic conditions, suggesting its ability for autotrophic growth [23].…”

Section: Nitrification and Denitrificationmentioning

confidence: 99%

“…Acidovorax is a Gram-negative bacterium which has the ability of using both acetate and hydrogen for denitrification [29]. Denitrifying bacteria, similar to Acidovorax and Azoarcus, a facultatively anaerobic, mesophilic, and Gram-negative bacterium with the ability of growing with a variety of organic substrates [26], have been identified under mixotrophic denitrifying conditions [27]. Denitrifying species of Acidovorax spp.…”

Section: Nitrification and Denitrificationmentioning

confidence: 99%

“…C27 for conducting both organotrophic and autotrophic denitrification has been reported [22]. On the other hand, Thiobacillus denitrificans, an obligate autotrophy and facultative anaerobic bacterium, which can use elemental sulfur as an electron donor, has been isolated from natural environments, manmade environments [17], and denitrifying reactors operated under mixotrophic conditions [27].…”

Section: Nitrification and Denitrificationmentioning

confidence: 99%

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Denitrification in the Presence of Chlorophenols: Progress and Prospects

Martínez‐Gutiérrez¹,

Texier²,

Cuervo-López³

et al. 2017

Nitrification and Denitrification

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Diverse industrial effluents may contain recalcitrant compounds such as chlorophenols. Besides, excessive use of pesticides in agriculture is a major cause of the appearance of chlorophenols in surface and groundwater. To mitigate and control the effects of chlorophenols in the environment, various methods have been developed for their elimination. Biological processes represent a sustainable and economical alternative that can lead to the mineralization of chlorophenols and be effective for the removal of these pollutants from different water bodies, such as rivers, groundwater, and wastewater. Some studies have reported that chlorophenols mineralization and nitrate reduction may simultaneously be performed. Other works have suggested that a reductive dechlorination occurs such as the first step and later, the phenol formed is subsequently mineralized by denitrification. However, the published information can be confusing as the denitrifying process is often associated with the sole nitrate consumption without corroborating the total reduction of nitrate to N 2 . Additionally, there are alternative systems that combine biological process with a chemical or electrochemical process for chlorophenols removal. This chapter focuses on the advances accomplished in the study of the removal of chlorophenols under denitrifying conditions with the aim of having a clearer panorama of the treatment alternatives that can be applied for treatment of this type of effluents.

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“…For instance, secondary effluents from municipal wastewater treatment plants (WWTPs) contain insufficient organic carbon for tertiary denitrification; the application of HAD in advanced treatment can reduce TN emission by utilizing both the small amount of organics in the secondary effluent and inorganic electron donors without the need for organic dosing. In previous studies, higher denitrification rates were achieved when limited organic carbon sources (liquid carbon, woodchips, and sludge) were subjected to sulfur- and ferrous-based autotrophic denitrification. , Therefore, to resolve complications with TN removal from organic-limited water and wastewater, it is essential to study the effects of organic carbon on autotrophic denitrification processes and to improve TN removal performance via the HAD process.…”

Section: Introductionmentioning

confidence: 99%

Stable-Isotope Probing Reveals the Activity and Function of Autotrophic and Heterotrophic Denitrifiers in Nitrate Removal from Organic-Limited Wastewater

Xing

et al. 2018

Environ. Sci. Technol.

112

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Combined heterotrophic and autotrophic denitrification (HAD) is a sustainable and practical method for removing nitrate from organic-limited wastewater. However, the active microorganisms responsible for denitrification in wastewater treatment have not been clearly identified. In this study, a combined microelectrolysis, heterotrophic, and autotrophic denitrification (CEHAD) process was established. DNA-based stable isotope probing was employed to identify the active denitrifiers in reactors fed with either C-labeled inorganic or organic carbon sources. The total nitrogen removal efficiencies reached 87.2-92.8% at a low organic carbon concentration (20 mg/L COD). Real-time polymerase chain reaction of the nirS gene as a function of the DNA buoyant density following the ultracentrifugation of the total DNA indicated markedC-labeling of active denitrifiers. High-throughput sequencing of the fractionated DNA in HCO/CHCOO-fed and HCO/CHCOO-fed reactors revealed that Thermomonas-like phylotypes were labeled by C-bicarbonate, while Thauera-like and Comamonas-like phylotypes were labeled byC-acetate. Meanwhile, Arenimonas-like and Rubellimicrobium-like phylotypes were recovered in the "heavy" DNA fractions from both reactors. These results suggest that nitrate removal in CEHAD is catalyzed by various active microorganisms, including autotrophs, heterotrophs, and mixotrophs. Our findings provide a better understanding of the mechanism of nitrogen removal from organic-limited water and wastewater and can be applied to further optimize such processes.

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Impact of operating condition on the denitrifying bacterial community structure in a 3DBER-SAD reactor

Cited by 12 publications

References 31 publications

Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor

Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor

Denitrification in the Presence of Chlorophenols: Progress and Prospects

Stable-Isotope Probing Reveals the Activity and Function of Autotrophic and Heterotrophic Denitrifiers in Nitrate Removal from Organic-Limited Wastewater

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