2017
DOI: 10.1016/j.enzmictec.2017.07.004
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Impact of orientation of carbohydrate binding modules family 22 and 6 on the catalytic activity of Thermotoga maritima xylanase XynB

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Cited by 19 publications
(3 citation statements)
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“…Our results suggest that the mechanism of EndoS2 relies on an interplay between its GH domain and CBM. Many endoglycosidases have CBMs appended to them; however, they are usually appended in seemingly random orientations relative to the GH domain, and only a few studies have investigated how the orientation of the CBM affects the activity of the GH domain. It is tempting to speculate that the orientation of the CBM affects EndoS2 activity for several reasons. First, the GH and CBM are located on opposite tips of the “V-shaped” enzyme, with their glycan-binding surfaces pointing inward.…”
Section: Discussionmentioning
confidence: 99%
“…Our results suggest that the mechanism of EndoS2 relies on an interplay between its GH domain and CBM. Many endoglycosidases have CBMs appended to them; however, they are usually appended in seemingly random orientations relative to the GH domain, and only a few studies have investigated how the orientation of the CBM affects the activity of the GH domain. It is tempting to speculate that the orientation of the CBM affects EndoS2 activity for several reasons. First, the GH and CBM are located on opposite tips of the “V-shaped” enzyme, with their glycan-binding surfaces pointing inward.…”
Section: Discussionmentioning
confidence: 99%
“…The designed linker peptide will interfere with the thermostability [ 27 , 28 , 29 ] and enzymatic activity [ 17 , 27 ] of adjacent domains. As previously reported, the flexibility of the linker region is of great significance for enzyme activity [ 30 , 31 , 32 ], so we used a natural 22-residue linker peptide (PEVLPPLPKESRISEGEAVVVG) to connect these domains. The linker we selected contains four Pro and two Ser residues, which can form flexible hinge regions and aid in creating an active spatial conformation between different domains [ 1 , 25 ].…”
Section: Discussionmentioning
confidence: 99%
“…However, it has been reported that fusion with CBMs may have different effects on different enzymes and the effects may also vary with different CBMs because of their divergent structures and properties. For instance, an increase in activity of Thermotoga maritima xylanase XynB was reported with the addition of CBM6 and CBM22 (Tajwar et al, 2017). In contrast, the addition of CBM6 to xylanase XynZ from Clostridium thermocellum resulted in decreased activity (Khan et al, 2013).…”
Section: Introductionmentioning
confidence: 99%