Impact of Origin and Biological Source on Chemical Composition, Anticholinesterase and Antioxidant Properties of Some St. John’s Wort Species (Hypericum spp., Hypericaceae) from the Central Balkans

Abstract

Abstract:The study shows the influence of the origin of plant material and biological source on the in vitro antioxidant (neutralization of DPPH and OH radical, nitric oxide, and inhibition of lipid peroxidation) and anticholinesterase activity of chemically characterized and quantified ethanol extracts of ten St. John's wort samples. The investigated samples were: five Hypericum perforatum species representatives collected at different localities, one commercial sample of Hyperici herba purchased at a local market and four Hypericum species autochtonous to the Balkan Peninsula (H. maculatum subsp. immaculatum, H. olympicum, H. richeri subsp. grisebachii and H. barbatum). All the examined extracts exhibited notable antioxidant potential, but in most of the cases indigenous Hypericum species expressed stronger effects compared to the original source of the drug, H. perforatum. The changes in the content of phenolic compounds, especially flavonoids, hyperforin and hypericin, related to the source of the drug affected the investigated activities. Since all of the investigated species have shown prominent inhibition of

Optimisation of organic solvent-free polyphenol extraction from Hypericum triquetrifolium Turra using Box–Behnken experimental design and kinetics

“…This was in line with the results on the Y TP and consisted clear evidence that differences in Y TP were reflected on the antioxidant properties of the extracts. This assumption is in accordance with previous examinations on various Hypericum species, where it was shown that extracts with higher polyphenolic load also exhibited stronger antioxidant effects [24,25]. Similar results were reported for several Hypericum taxa [26][27][28].…”

Natural Products for the Prevention and Treatment of Hangover and Alcohol Use Disorder

“…These results suggest that HPE could have some beneficial effects on alcohol withdrawal syndrome in people [102]. Hypericum perforatum exhibits remarkable antioxidant potential due to high content of phenolic compounds, especially flavonoids, hyperforin and hypericin [103]. Hyperforin has NMDA-receptor-antagonistic and potential neuroprotective effects in vitro which might provide therapeutic effectiveness in the relapse prevention of alcohol use disorders [103].…”

“…Hypericum perforatum exhibits remarkable antioxidant potential due to high content of phenolic compounds, especially flavonoids, hyperforin and hypericin [103]. Hyperforin has NMDA-receptor-antagonistic and potential neuroprotective effects in vitro which might provide therapeutic effectiveness in the relapse prevention of alcohol use disorders [103]. In addition, hyperforin could reduce alcohol intake more effectively than hypericin in alcohol-dependent mice [104].…”

The efficacy and mechanism of apoptosis induction by hypericin-mediated sonodynamic therapy in THP-1 macrophages

“…1,19,21 The polycyclic dione hypericin (HY), which is one of the active ingredients of St John's wort, is a photosensitizer that accumulates in dark glands found in flowers and leaves. [22][23][24] HY has been proposed as a highly promising photosensitizer due to its minimal toxicity, tumor high selectivity, high clearance from the host body, much singlet oxygen production and anti-inflammatory properties, as HY is able to induce the apoptosis of human lens epithelial cells, nasopharyngeal carcinoma cells, and U87 glioma cells 20,23,[25][26][27][28] during the rapid healing process after PDT. However, the effectiveness of HY as a potential sonosensitizer for SDT remains relatively unknown.…”