Impact of oxidative stress on oocyte competence for in vitro embryo production programs

Soto-Heras, Sandra; Paramio, M. T.

doi:10.1016/j.rvsc.2020.07.013

Cited by 81 publications

(45 citation statements)

References 137 publications

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“…These studies have unveiled critical roles for Sirtuins during oocyte maturation. Sirtuins protect oocytes from oxidative stress, a major problem encountered during in vitro maturation techniques (Agarwal et al, 2012; Soto‐Heras & Paramio, 2020). Sirtuins also promote epigenetic changes during oocyte maturation but what are the readers of these epigenetic marks and the downstream biological functions remain mostly unknown.…”

Section: Discussionmentioning

confidence: 99%

Sirtuins in female meiosis and in reproductive longevity

Vázquez

Vaquero

Schindler

2020

Molecular Reproduction Devel

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Transmission of genetic material through high-quality gametes to progeny requires accurate homologous chromosome recombination and segregation during meiosis. A failure to accomplish these processes can have major consequences in reproductive health, including infertility, and development disorders in offspring. Sirtuins, a family of NAD +-dependent protein deacetylases and ADP-ribosyltransferases, play key roles in genome maintenance, metabolism, and aging. In recent years, Sirtuins have | VAZQUEZ ET AL.

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Section: Discussionmentioning

confidence: 99%

Sirtuins in female meiosis and in reproductive longevity

Vázquez

Vaquero

Schindler

2020

Molecular Reproduction Devel

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“…However, ROS does not only have a negative effect on cells. It is also demonstrated that ROS is involved in the regulation of meiotic maturation and that a low level of ROS is essential for proper meiotic maturation (Soto-Herasa & Paramio, 2020). Oocyte cultivation in a medium containing high concentrations of ROS scavengers can lead to the inhibition of meiotic maturation (Tiwari et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Proper meiotic maturation in in vitro conditions is crucial for the development of biotechnological methods and the use of assisted reproduction methods. A balanced redox state is important for proper oocyte development (Soto-Herasa & Paramio, 2020), but in vitro cultivation systems of oocytes are characterized by increased oxidative stress (Khazaei & Aghaz, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Antioxidant supplementation of the culture medium can be used to avoid the harmful effect of oxidative stress. For example, the non-enzymatic antioxidant melatonin has been shown to improve oocyte maturation, fertilization rate, and rate of blastocyst formation (Tamura et al, 2020;Soto-Herasa & Paramio, 2020). The use of antioxidants can therefore ameliorate in vitro cultivation conditions, but novel substances are still needed to reduce oxidative stress during in vitro oocyte cultivation to improve oocyte quality.…”

Section: Introductionmentioning

confidence: 99%

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The effect of carbon monoxide on meiotic maturation of porcine oocytes

Němeček

Eva

Petr

et al. 2021

PeerJ

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Oxidative stress impairs the correct course of meiotic maturation, and it is known that the oocytes are exposed to increased oxidative stress during meiotic maturation in in vitro conditions. Thus, reduction of oxidative stress can lead to improved quality of cultured oocytes. The gasotransmitter carbon monoxide (CO) has a cytoprotective effect in somatic cells. The CO is produced in cells by the enzyme heme oxygenase (HO) and the heme oxygenase/carbon monoxide (HO/CO) pathway has been shown to have an antioxidant effect in somatic cells. It has not yet been investigated whether the CO has an antioxidant effect in oocytes as well. We assessed the level of expression of HO mRNA, using reverse transcription polymerase chain reaction. The HO protein localization was evaluated by the immunocytochemical method. The influence of CO or HO inhibition on meiotic maturation was evaluated in oocytes cultured in a culture medium containing CO donor (CORM-2 or CORM-A1) or HO inhibitor Zn-protoporphyrin IX (Zn-PP IX). Detection of reactive oxygen species (ROS) was performed using the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate. We demonstrated the expression of mRNA and proteins of both HO isoforms in porcine oocytes during meiotic maturation. The inhibition of HO enzymes by Zn-PP IX did not affect meiotic maturation. CO delivered by CORM-2 or CORM-A1 donors led to a reduction in the level of ROS in the oocytes during meiotic maturation. However, exogenously delivered CO also inhibited meiotic maturation, especially at higher concentrations. In summary, the CO signaling molecule has antioxidant properties in porcine oocytes and may also be involved in the regulation of meiotic maturation.

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Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "The effect of carbon monoxide on meiotic maturation of porcine oocytes (v0.1)"

Lopez¹

2021

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Oxidative stress impairs the correct course of meiotic maturation and it is known that the oocytes are exposed to increased oxidative stress during meiotic maturation in in vitro conditions. Thus, reduction of oxidative stress can lead to improved quality of cultured oocytes. The gasotransmitter carbon monoxide (CO) has a cytoprotective effect in somatic cells. The CO is produced in cells by the enzyme heme oxygenase (HO) and the heme oxygenase/carbon monoxide (HO/CO) pathway has been shown to have an antioxidant effect in somatic cells. It has not yet been known whether the CO has an antioxidant effect in oocytes as well. We assessed the level of expression of HO mRNA, using reverse transcription polymerase chain reaction. The HO protein localization was evaluated by the immunocytochemical method. The influence of CO or HO inhibition on meiotic maturation was evaluated in oocytes cultured in a culture medium containing CO donor (CORM-2 or CORM-A1) or HO inhibitor Zn-protoporphyrin IX (Zn-PP IX). Detection of reactive oxygen species (ROS) was performed using the oxidant-sensing probe 2',7'dichlorodihydrofluorescein diacetate. We demonstrated the expression of mRNA and proteins of both HO isoforms in porcine oocytes during meiotic maturation. The inhibition of HO enzymes by Zn-PP IX did not affect meiotic maturation. CO delivered by CORM-2 or CORM-A1 donors led to a reduction in the level of ROS in the oocytes during meiotic maturation. However, exogenously delivered CO also inhibited meiotic maturation, especially at higher concentrations. In summary, the CO signaling molecule has antioxidant properties in porcine oocytes and may also be involved in the regulation of meiotic maturation.

show abstract

Impact of oxidative stress on oocyte competence for in vitro embryo production programs

Cited by 81 publications

References 137 publications

Sirtuins in female meiosis and in reproductive longevity

Sirtuins in female meiosis and in reproductive longevity

The effect of carbon monoxide on meiotic maturation of porcine oocytes

Peer Review #2 of "The effect of carbon monoxide on meiotic maturation of porcine oocytes (v0.1)"

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