Impact of particle size gradients on the apparent efficiency of chromatographic columns

Preparation of ion exchange columns with longitudinal stationary phase gradients

“…Investigation of capacity gradients is of potential interest because an increasing capacity gradient should result in peak focusing. As with the particle size gradient study of Fekete et al [17], capacity gradients are not expected to provide any efficiency benefit under isocratic conditions. Under such conditions the peak focusing effect of an increasing capacity gradient is offset by the relatively slow release of the band due to the high capacity at the outlet of the column.…”

“…Fekete et al published a theoretical study of the impact of a particle size gradient on chromatographic efficiency [17]. They concluded that under isocratic conditions a particle size gradient would provide no performance benefits.…”

Synthesis of Hybrid Monolithic Columns Using a Click Chemistry Reaction for Application in Capillary Liquid Chromatography

“…Efficiency values above 20000 plates m -1 for capillary monolithic columns are considered satisfactory for chromatographic separations, 37 although the literature presents values between 90000-110000 plates m -1 for other types of hybrid monoliths. [38][39][40] For asymmetry, values between 0.9 and 1.2 are considered satisfactory according to the United States Pharmacopeia (USP). 41 To compare the columns having monoliths 3 and 4, a mixture of alkylbenzenes was used, as seen in Figure 6.…”