Impact of Pb on Chlamydomonas reinhardtii at Physiological and Transcriptional Levels

Zheng, Canqi; Aslam, Muhammad; Liu, Xiaojuan; Du, Hong; Xie, Xihui; Jia, Haojie; Huang, Nan; Tang, Kaiming; Yang, Yingquan; Li, Ping

doi:10.3389/fmicb.2020.01443

Cited by 28 publications

(20 citation statements)

References 86 publications

(99 reference statements)

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“…Furthermore, the identified N-glycoproteins were all annotated by Clusters of Orthologous Group/Eukaryote Orthologous Group (COG/KOG) database ( Figure 3A ; Zheng et al, 2020 ). A total of 285 N-glycosylated proteins were annotated in COG/KOG database.…”

Section: Resultsmentioning

confidence: 99%

Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum

Xie

Chen

et al. 2021

Front. Plant Sci.

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N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae. Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum. In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum. These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum. Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway. Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans. High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue. Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue. The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae. The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.

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Section: Resultsmentioning

confidence: 99%

Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum

Xie

Chen

et al. 2021

Front. Plant Sci.

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“…Error bars indicate SD for three biological replicates. Cell density was referenced from our recent article ( Zheng et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…On the third day, algal cells under the control and the lead stresses were used to do transcriptomic analysis. The transcriptomic data were referenced from our recent article ( Zheng et al, 2020 ). The sequence reads had already been available by the National Center for Biotechnology Information sequence read archives (SRR10269729, SRR10269730, SRR10269727, SRR10269728, SRR10269731, and SRR10269732).…”

Section: Methodsmentioning

confidence: 99%

Endoplasmic Reticulum-Mediated Protein Quality Control and Endoplasmic Reticulum-Associated Degradation Pathway Explain the Reduction of N-glycoprotein Level Under the Lead Stress

Zheng

Aslam

et al. 2021

Front. Plant Sci.

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Different anthropogenic activities result in the continuous increase of metal lead (Pb) in the environment and adversely affect living organisms. Therefore, it is important to investigate the tolerance mechanism in a model organism. Chlamydomonas reinhardtii is an important green eukaryotic model microalga for studying different kinds of biological questions. In this study, the responses of C. reinhardtii were revealed via a comprehensive approach, including physiological, genomic, transcriptomic, glycomic, and bioinformatic techniques. Physiological results showed that the growth rate and soluble protein content were significantly reduced under the high lead stress. Also, the results obtained from the genomic and transcriptomic analyses presented that the endoplasmic reticulum-mediated protein quality control (ERQC) system and endoplasmic reticulum-associated degradation (ERAD) pathway were activated under the third day of high lead stress. The unique upregulated protein disulfide isomerase genes on the ERQC system were proposed to be important for the protein level and protein quality control. The accumulation of specific N-glycans indicated that specific N-glycosylation of proteins might alter the biological functions of proteins to alleviate the Pb stress in alga and/or lead to the degradation of incomplete/misfolded proteins. At the same time, it was observed that genes involved in each process of ERAD were upregulated, suggesting that the ERAD pathway was activated to assist the degradation of incomplete/misfolded proteins. Therefore, it is reasonable to speculate that the reduction of protein level under the high lead stress was related to the activated ERQC system and QRAD pathway. Our findings will provide a solid and reliable foundation and a proposed ERAD working model for further in-depth study of the ERQC system and ERAD pathway under the Pb stress and even other biotic and abiotic stresses.

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“…Recently, Zheng et al. (2020) analyzed the genes involved in the potential signaling pathways in Chlamydomonas reinhardtii and their ultimate responses to phytohormones. They annotated two genes putatively responding to ABA, the nucleotide‐binding gene (Cre09.g392350) and PPP13 (Cre03.g186950).…”

Section: Discussionmentioning

confidence: 99%

Exogenous Abscisic Acid Confers Salinity Tolerance in Chlamydomonas reinhardtii During Its Life Cycle

Abu‐Ghosh

Iluz

Dubinsky

et al. 2021

Journal of Phycology

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The plant hormone abscisic acid (ABA) coordinates responses to environmental signals with developmental changes and is important for stress resilience and crop yield. However, fundamental questions remain about how this phytohormone affects microalgal growth and stress regulation throughout the different stages of their life cycle. In this study, the effects of ABA on the physiology of the freshwater microalga Chlamydomonas reinhardtii at its different life cycle stages were investigated. Exogenously added ABA enhanced the growth and photosynthesis of C. reinhardtii during the vegetative stage. The hormone also increased the tolerance of this alga to high‐salinity stress during gamete formation under nutrient depletion, as well as it extended their survival. We show that the level of reactive oxygen species (ROS) generated in the ABA‐treated cells was significantly less than that in the untreated cells under inhibiting NaCl concentrations. Cell size examination showed that ABA prevents cells from forming palmella when exposed to high salinity. All together, these results suggest that ABA can support the vitality and survival of C. reinhardtii under high salt conditions.

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Impact of Pb on Chlamydomonas reinhardtii at Physiological and Transcriptional Levels

Cited by 28 publications

References 86 publications

Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum

Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum

Endoplasmic Reticulum-Mediated Protein Quality Control and Endoplasmic Reticulum-Associated Degradation Pathway Explain the Reduction of N-glycoprotein Level Under the Lead Stress

Exogenous Abscisic Acid Confers Salinity Tolerance in Chlamydomonas reinhardtii During Its Life Cycle

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