Impact of PDMS surface treatment in cell-mechanics applications

Dogru, Sedat; Aydemir, Duygu; Salman, Naveed; Ulusu, Nuriye Nuray; Alaca, B. Erdem

doi:10.1016/j.jmbbm.2019.103538

Cited by 10 publications

(6 citation statements)

References 48 publications

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“…39 The polymer chains in PDMS are all chemically linked (covalently bonded) compared to those in TPU and PMMA where both weaker physical (i.e., hydrogen bonding) and chemical cross-linking exist. 23 Exposure of PDMS to a force within its elastic limit for a short duration of time leads to full elastic recovery, 40 whereas with TPU, there is greater rearrangement of polymer chains under a force and hence the slower recovery. Increasing the weight percentage of TPU in the coaxial fibers due to the increasing flow rate in the core will also mean a more gradual elastic recovery as is shown with the samples.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Fabrication of Nanocomposites with High Elasticity and Strength for the Load-Bearing Layer of Small-Diameter Vascular Grafts

Zizhou

Khoshmanesh

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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Compliance mismatch of commercially available artificial grafts, where the artificial graft and the native vessel are subject to different radial expansions, is a major issue that results in graft occlusion after implantation. A human artery possesses a nonlinear mechanical response to pulsatile pressure due to its nonlinear viscoelastic nature, which is difficult to replicate in artificial graft fabrication. Here, we fabricated nanocomposites with nonlinear mechanical responses for potential application as the load-bearing layer of vascular grafts, based on a poly(dimethylsiloxane) (PDMS)-casted nanofibrous film. The nanofibers consisted of a core–sheath structure with a PDMS elastomer reinforced with poly(methyl methacrylate) (PMMA) nanofibers as the sheath and thermoplastic polyurethane (TPU) elastomer as the core. The surface morphology and chemical composition together with the crystalline structure of the nanocomposites were characterized, and dynamic mechanical analysis was performed to select the graft with the most suitable properties as the load-bearing layer of a small-diameter vascular graft. The presence of the stiff polymer PMMA and elastic polymer TPU in the PMMA/PDMS/TPU combination resulted in a delayed dissipation of energy after exposure to a force corresponding to 180 mm Hg. Casting the PDMS/PMMA/TPU nanofibrous mat into a nanocomposite film improved the ultimate tensile strength of PDMS without compromising its elasticity. The compliance values of the nanocomposites were also found to be a close match to those of the greater saphenous vein, demonstrating a great potential of the nanocomposites as the load-bearing layer in a biostable vascular graft.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Fabrication of Nanocomposites with High Elasticity and Strength for the Load-Bearing Layer of Small-Diameter Vascular Grafts

Zizhou

Khoshmanesh

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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“…[92][93][94][95][96] In the eld of mechanobiology, surface mechanical forces play a key role in maintaining tissue functions of the cells developed on PDMS substrates during and aer the developmental stage. 5 As a result, the impairment of mechanical forces on the substrate can lead to various diseases such as cardiac hypertrophy, 97,98 arthritis, 99-101 asthma, 102 osteoporosis, 103 deafness, 104,105 atherosclerosis, 106 cancer, 107 glaucoma, 108 and muscular dystrophy. 109 To quantify the surface and intracellular forces involved during tissue growth, PDMS, attributing to its tuneable mechanical properties and high refractive index, is one of the most used substrate materials to mark force elds in traction force microscopy (TFM) and microfabricated post array detectors (mPADs) in mechanobiology laboratories.…”

Section: Discussionmentioning

confidence: 99%

“…3,4 As one of the most widely used elastomers in the eld of cell mechanics, PDMS is subjected to several surface treatments such as plasma oxidation and UV sterilization, that directly affect the mechanical properties of PDMS. 5 The minor changes imparted to the surface mechanical properties of PDMS are reported to cause signicant errors in quantifying cellular traction forces. [6][7][8] Since PDMS is a so polymer with low elastic modulus in the range of 1-3 MPa in its unmodied conditions, [9][10][11] attempts to improve its local mechanical properties using nanoscale reinforcements are prominent.…”

Section: Introductionmentioning

confidence: 99%

“…3,4 As one of the most widely used elastomers in the field of cell mechanics, PDMS is subjected to several surface treatments such as plasma oxidation and UV sterilization, that directly affect the mechanical properties of PDMS. 5 The minor changes imparted to the surface mechanical properties of PDMS are reported to cause significant errors in quantifying cellular traction forces. 6–8…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the surface mechanical properties of functionalized single-walled carbon nanotube (SWCNT) reinforced PDMS nanocomposites using nanoindentation analysis

Ananthasubramanian,

Sahay,

Raghavan

2024

RSC Adv.

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“…Conical indentation tests on the flat samples and the spherical samples were conducted to examine the modified models as discussed before. The Poisson's ratio of PDMS was reported to be 0.40 to 0.50 [64,65] and a value of 0.49 was adopted in this study. The Young's modulus obtained from the indentation tests of the flat samples is 0.99 ± 0.08 MPa at a large deformation using the modified model, which is consistent with the results (1.08 ± 0.1 MPa) within measurement error determined by the original Sneddon model at a small deformation (i.e.…”

Section: Experimental Validation For Modified Sneddon Modelmentioning

confidence: 99%

A modified Sneddon model for the contact between conical indenters and spherical samples

Han

Chen

2021

Journal of Materials Research

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Indentation techniques have proven to be effective to characterize the mechanical properties of materials. For the elastic deformation, the commonly used models are Hertz model and Sneddon model. However, neither of them works for indenting the spherical samples using the pyramid or conical indenter. Therefore, one modified Sneddon model has been developed to determine the Young’s modulus of spherical samples from indentation results. In this study, the effects of sample diameter and indenter angles on indentation tests were investigated by finite element method (FEM). The empirical correction parameters in the new mathematical model were introduced based on dimensional analysis and determined by the numerical fitting to FEM results. Experimental tests with different conical indenters have demonstrated that the new model is capable to reliably determine the Young’s modulus of the spherical samples. The new model can fill the gap of the contact mechanics and enrich the experimental solid mechanics for the interpretation of indentation results. Graphic abstract

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Impact of PDMS surface treatment in cell-mechanics applications

Cited by 10 publications

References 48 publications

Fabrication of Nanocomposites with High Elasticity and Strength for the Load-Bearing Layer of Small-Diameter Vascular Grafts

Fabrication of Nanocomposites with High Elasticity and Strength for the Load-Bearing Layer of Small-Diameter Vascular Grafts

Investigation of the surface mechanical properties of functionalized single-walled carbon nanotube (SWCNT) reinforced PDMS nanocomposites using nanoindentation analysis

A modified Sneddon model for the contact between conical indenters and spherical samples

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