The review presents data on chemical-analytical methods of PCDD, PCDF determination and biotesting methods used for toxicological assessment of pollutants. Distribution of PCDDs, PCDFs on the territory of Moscow is analyzed. Analysis of publications of Russian and foreign authors showed that the currently existing methods of biotesting are in fact untested for dioxins. In order to quickly establish the toxicological effect of persistent organic pollutants in soil, it is suggested to use biotesting methods, with inclusion of test organisms representing the main trophic levels of ecosystems: producers, consumers, and decomposers.