Impact of photoperiod on uterine redox/inflammatory and metabolic status of golden hamster, Mesocricetus auratus

Abstract

Photoperiod modulates reproductive physiology at multiple levels in seasonally breeding animals. Golden hamsters are long-day breeders that diminish their fertility during the short days. Photoperiod is known to regulate hormonal milieu and uterus is a hormone-sensitive dynamic tissue. However, there is lack of molecular insight regarding the impact of photoperiod on uterine physiology with respect to redox and metabolic status in Mesocricetus auratus. We evaluated the impact of photoperiod onprogesterone [P4], melatonin, and insulin), their receptor expressions and key markers associated with redox (SIRT-1/FOXO-1), inflammatory (NFĸB/COX-2) and metabolic (IR/GLUT4) status in uterus. Adult female golden hamsters were exposed to different photoperiodic regimes, that is, short photoperiod (SP; 8L:16D) and long photoperiod (LP; 16L:8D) for 12 weeks. SP drastically decreased peripheral hormone profiles (T3, T4, E2, and P4) and compromised uterine histoarchitecture when compared with LP-exposed hamsters. Further, SP markedly decreased thyroid hormone receptor-α (TRα), insulin receptor, and glucose uptake transporter-4 (GLUT-4) expressions in uterus. We noted enhanced uterine oxidative (increased MDA and decreased SOD/CAT levels), SIRT-1/FOXO-1 expression and inflammatory (NFĸB/COX-2) load in SP condition. Further, elevated levels of circulatory insulin, melatonin, and its receptor (MT-1) expression in uterus was noted under SP condition. Thus, we may suggest that photoperiod might regulate uterine seasonality through modulation of local hormonal and redox/metabolic homeostasis thereby may restrict offspring bearing capacity under short days.

BPS‐induced ovarian dysfunction: Protective actions of melatonin via modulation of SIRT‐1/Nrf2/NFĸB and IR/PI3K/pAkt/GLUT‐4 expressions in adult golden hamster

“…Hamsters were kept under photoperiodic regime of 12.5 h light/11.5 h dark cycle in which they behave as continuous breeders. [36][37][38] All the experiments conducted for our study were in accordance with the guidelines set by the Committee for the Purpose of Supervision and Control on Experiments on Animals (CPSCEA), Government of India.…”

“…The thorough methodology for immunohistochemical detection of PCNA in ovarian sections was carried out using protocol described elsewhere. 19,37 The sections were then examined under Nikon Eclipse E200 research microscope with NIS-Element D software in randomly selected sections.…”

Effects of Seasonal Photoperiod on Growth, Lipid Metabolism, and Antioxidant Response in the Huanghe Carp (Cyprinus carpio haematopterus)

“…Some studies have also demonstrated that FOXO1 plays a key role in autophagy regulation and can upregulate the expression of autophagy-related genes [16]. FOXO signaling plays an important role in a study of photoperiodic effects on uterine metabolism in the golden hamster (Mesocricetus auratus), where photoperiod regulates uterine function-related seasonality through redox/metabolic homeostasis [17]. However, whether FOXO is involved and the role played under photoperiod-induced physiological changes in carp has not been studied.…”