Impact of platelet count on results obtained from multiple electrode platelet aggregometry (Multiplate™)

Aa, Hanke; Roberg, K.A.; Monaca, Enrico; Sellmann, Timur; Weber, Cornelius; Rahe‐Meyer, Niels; Görlinger, Klaus

doi:10.1186/2047-783x-15-5-214

Cited by 144 publications

(99 citation statements)

References 19 publications

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“…These results extend our recent findings of a very high reproducibility of the VerifyNow Ò assay compared with MEA and other platelet function tests [23]. Moreover, a very recent study on 20 healthy volunteers reported that results obtained using MEA are significantly affected by variations in platelet count below the normal range [24]. Our study extends these findings to CAD patients with normal platelet counts.…”

Section: Platelet Turnover and Platelet Aggregation In Whole Bloodsupporting

confidence: 90%

Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease

Grove¹,

Hvas

Mortensen³

et al. 2011

Journal of Thrombosis and Haemostasis

133

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To cite this article: Grove EL, Hvas AM, Mortensen SB, Larsen SB, Kristensen SD. Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease. J Thromb Haemost 2011; 9: 185-91. Summary.Background: Previous studies have demonstrated considerable variation in the antiplatelet effect of aspirin. Objectives: To investigate the impact of platelet turnover on the antiplatelet effect of aspirin in patients with stable coronary artery disease (CAD) and to identify determinants of platelet turnover. Methods: Platelet turnover was evaluated by measurements of immature platelets and thrombopoietin in 177 stable CAD patients on aspirin monotherapy, including 85 type 2 diabetics and 92 non-diabetics. Whole blood platelet aggregation was determined using the VerifyNow Ò Aspirin test and multiple electrode aggregometry (MEA, Multiplate , P = 0.034). In a multivariate regression analysis, immature platelet levels were determined by thrombopoietin levels (P < 0.001), smoking (P = 0.020) and type 2 diabetes (P = 0.042). Conclusions: The antiplatelet effect of aspirin was reduced in CAD patients with an increased platelet turnover. Once-daily dosing of aspirin might not suffice to adequately inhibit platelet aggregation in patients with an increased platelet turnover.

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Section: Platelet Turnover and Platelet Aggregation In Whole Bloodsupporting

confidence: 90%

Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease

Grove¹,

Hvas

Mortensen³

et al. 2011

Journal of Thrombosis and Haemostasis

133

View full text Add to dashboard Cite

show abstract

“…Mengistu blood aggregometry at lower platelet concentrations [19,20]. Two more studies also reported correlations between platelet count and extent of platelet aggregation on MEA [18,21]. However, the latter results are difficult to interpret as the counts were adjusted either by addition of PPP [21], which is known to inhibit platelet aggregation [17], or with dilution with Tyrode's buffer [18].…”

Section: Discussionmentioning

confidence: 99%

Comparison of multiple electrode aggregometry with lumi‐aggregometry for the diagnosis of patients with mild bleeding disorders

Ghaithi

Drake

Watson

et al. 2017

Journal of Thrombosis and Haemostasis

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Background Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing preanalytical errors associated with preparation of platelet-rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents (e.g. aspirin and clopidogrel). Objective Our current study sought to evaluate the ability of MEA in diagnosing patients with mild bleeding disorders by comparison with light transmission lumi-aggregometry (lumi-LTA). Methods Forty healthy subjects and 109 patients with a clinical diagnosis of a mild bleeding disorder were recruited into the UK Genotyping and Phenotyping of Platelets study (GAPP, ISRCTN 77951167). MEA was performed on whole blood using one or two concentrations of ADP, PAR-1 peptide, arachidonic acid and collagen. Lumi-LTA was performed in PRP using several concentrations of ADP, adrenaline, arachidonic acid, collagen, PAR-1 peptide and ristocetin. Results Of 109 patients tested, 54 (49%) patients gave abnormal responses by lumi-LTA to one or more agonists. In contrast, only 16 (15%) patients were shown to have abnormal responses to one or more agonists by MEA. Conclusions In this study we showed that MEA is less sensitive in identifying patients with abnormal platelet function relative to lumi-LTA.

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“…Platelet aggregation was measured using a lumi-aggregometer (Chrono-log Corp., Havertown, PA, USA) and was monitored for 8 min. The area under the aggregation curve was calculated by Image-Pro Plus software (Media Cybernetics, Silver Spring, MD, USA) for statistical analysis as previously described (Hanke et al, 2010).…”

Section: Platelet Aggregationmentioning

confidence: 99%

Aspirin enhances protective effect of fish oil against thrombosis and injury‐induced vascular remodelling

Gong

Lin

Piao

et al. 2015

British J Pharmacology

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Background and Purpose Although aspirin (acetylsalicylic acid) is commonly used to prevent ischaemic events in patients with coronary artery disease, many patients fail to respond to aspirin treatment. Dietary fish oil (FO), containing ω3 polyunsaturated fatty acids (PUFAs), has anti‐inflammatory and cardio‐protective properties, such as lowering cholesterol and modulating platelet activity. The objective of the present study was to investigate the potential additional effects of aspirin and FO on platelet activity and vascular response to injury. Experimental Approach Femoral arterial remodelling was induced by wire injury in mice. Platelet aggregation, and photochemical‐ and ferric chloride‐induced carotid artery thrombosis were employed to evaluate platelet function. Key Results FO treatment increased membrane ω3 PUFA incorporation, lowered plasma triglyceride and cholesterol levels, and reduced systolic BP in mice. FO or aspirin alone inhibited platelet aggregation; however, when combined, they exhibited synergistic suppression of platelet activity in mice, independent of COX‐1 inhibition. FO alone, but not aspirin, attenuated arterial neointimal growth in response to injury. Strikingly, a combination of FO and aspirin synergistically inhibited injury‐induced neointimal hyperplasia and reduced perivascular inflammatory reactions. Moreover, co‐administration of FO and aspirin decreased the expression of pro‐inflammatory cytokines and adhesion molecules in inflammatory cells. Consistently, a pro‐resolution lipid mediator‐Resolvin E1, was significantly elevated in plasma in FO/aspirin‐treated mice. Conclusions and Implications Co‐administration of FO and low‐dose aspirin may act synergistically to protect against thrombosis and injury‐induced vascular remodelling in mice. Our results support further investigation of adjuvant FO supplementation for patients with stable coronary artery disease. Linked Articles This article is part of a themed section on Chinese Innovation in Cardiovascular Drug Discovery. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2015.172.issue-23

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Impact of platelet count on results obtained from multiple electrode platelet aggregometry (Multiplate™)

Cited by 144 publications

References 19 publications

Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease

Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease

Comparison of multiple electrode aggregometry with lumi‐aggregometry for the diagnosis of patients with mild bleeding disorders

Aspirin enhances protective effect of fish oil against thrombosis and injury‐induced vascular remodelling

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