Impact of polymicrobial biofilms in catheter-associated urinary tract infections

Azevedo, Andreia S.; Almeida, Carina; Melo, L. F.; Azevedo, N. F.

doi:10.1080/1040841x.2016.1240656

Cited by 67 publications

(58 citation statements)

References 171 publications

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“…This observation is in line with earlier studies indicating that, upon exposure to stress, fungi initiate a response based on activating cell wall salvage pathways that compensate for cell wall damage as a rescue mechanism 96–100 including, for example, an increase in transcript abundance of MPKC in response to oxidative stress 102–104,116–118 . Since the formation of biofilms can per se lead to resistance against antimicrobial treatment owing to the decreased physical accessibility of the pathogens by drugs 89,119–121 , additional effects of microbial interactions within mixed biofilms affecting the protective mechanisms of individual pathogens may further enhance the resistance to therapy. The understanding of processes occurring during bacterial-fungal interactions (BFI) is therefore essential for the development of appropriate diagnostic and therapeutic approaches.…”

Section: Discussionmentioning

confidence: 99%

Klebsiella pneumoniae prevents spore germination and hyphal development of Aspergillus species

Nogueira

Pereira

Jenull

et al. 2019

Sci Rep

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Different bacteria and fungi live as commensal organisms as part of the human microbiota, but shifts to a pathogenic state potentially leading to septic infections commonly occur in immunocompromised individuals. Several studies have reported synergistic or antagonistic interactions between individual bacteria and fungi which might be of clinical relevance. Here, we present first evidence for the interaction between Klebsiella pneumoniae and several Aspergillus species including A. fumigatus, A. terreus, A. niger and A. flavus which cohabit in the lungs and the intestines. Microbiological and molecular methods were employed to investigate the interaction in vitro, and the results indicate that Klebsiella pneumoniae is able to prevent Aspergillus spp. spore germination and hyphal development. The inhibitory effect is reversible, as demonstrated by growth recovery of Aspergillus spp. upon inhibition or elimination of the bacteria, and is apparently dependent on the physical interaction with metabolically active bacteria. Molecular analysis of Klebsiella-Aspergillus interaction has shown upregulation of Aspergillus cell wall-related genes and downregulation of hyphae-related genes, suggesting that Klebsiella induces cell wall stress response mechanisms and suppresses filamentous growth. Characterization of polymicrobial interactions may provide the basis for improved clinical management of mixed infections by setting the stage for appropriate diagnostics and ultimately for optimized treatment strategies.

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Section: Discussionmentioning

confidence: 99%

Klebsiella pneumoniae prevents spore germination and hyphal development of Aspergillus species

Nogueira

Pereira

Jenull

et al. 2019

Sci Rep

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“…Frequencies higher than 1 are shown in a gray heat map, darker gray indicates higher frequency. Asterisk indicates common genera defined in the literature (Azevedo et al, 2017) .…”

Section: Resultsmentioning

confidence: 99%

Molecular Techniques Complement Culture-Based Assessment of Bacteria Composition in Mixed Biofilms of Urinary Tract Catheter-Related Samples

et al. 2019

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Urinary or ureteral catheter insertion remains one of the most common urological procedures, yet is considered a predisposing factor for urinary tract infection. Diverse bacterial consortia adhere to foreign body surfaces and create various difficult to treat biofilm structures. We analyzed 347 urinary catheter- and stent-related samples, treated with sonication, using both routine culture and broad-range 16S rDNA PCR followed by Denaturing Gradient Gel Electrophoresis and Sanger sequencing (PCR-DGGE-S). In 29 selected samples, 16S rRNA amplicon Illumina sequencing was performed. The results of all methods were compared. In 338 positive samples, from which 86.1% were polybacterial, 1,295 representatives of 153 unique OTUs were detected. Gram-positive microbes were found in 46.5 and 59.1% of catheter- and stent-related samples, respectively. PCR-DGGE-S was shown as a feasible method with higher overall specificity (95 vs. 85%, p < 0.01) though lower sensitivity (50 vs. 69%, p < 0.01) in comparison to standard culture. Molecular methods considerably widened a spectrum of microbes detected in biofilms, including the very prevalent emerging opportunistic pathogen Actinotignum schaalii. Using massive parallel sequencing as a reference method in selected specimens, culture combined with PCR-DGGE was shown to be an efficient and reliable tool for determining the composition of urinary catheter-related biofilms. This might be applicable particularly to immunocompromised patients, in whom catheter-colonizing bacteria may lead to severe infectious complications. For the first time, broad-range molecular detection sensitivity and specificity were evaluated in this setting. This study extends the knowledge of biofilm consortia composition by analyzing large urinary catheter and stent sample sets using both molecular and culture techniques, including the widest dataset of catheter-related samples characterized by 16S rRNA amplicon Illumina sequencing.

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“…Biofilm formation has serious clinical implications. Biofilm in the uroepithelium of the bladder has been suggested as the mechanism responsible for recurrent cystitis [ 10 , 45 ]. Moreover, CAUTIs are a serious problem during hospitalization, which is caused by a formation of a biofilm on the catheter [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) in reducing the motility and the biofilm formation of uropathogenic Escherichia coli

Ranfaing¹,

Dunyach-Rémy

Lavigne

et al. 2018

PLoS ONE

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One strategy to prevent urinary tract infections is the use of natural products such as cranberry (Vaccinium macrocarpon) and propolis. The objective of this study was to evaluate the impact of these products alone and combined on the motility and biofilm formation of a collection of representative uropathogenic Escherichia coli (UPEC). Motility was evaluated by the swarming and swimming capacity of the isolates in presence/absence of cranberry ± propolis. Early and late biofilm formation was observed with the Biofilm Ring test (BioFilm Control) and the crystal violet method. Cranberry alone was seen to have a variable effect on motility and biofilm formation unrelated to bacterial characteristics, but a reduced motility and biofilm formation was observed for all the isolates in the presence of cranberry + propolis. These results suggest that cranberry alone doesn’t work on all the E. coli strains and propolis potentiates the effect of cranberry on UPEC, representing a new strategy to prevent recurrent urinary tract infections.

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Impact of polymicrobial biofilms in catheter-associated urinary tract infections

Cited by 67 publications

References 171 publications

Klebsiella pneumoniae prevents spore germination and hyphal development of Aspergillus species

Klebsiella pneumoniae prevents spore germination and hyphal development of Aspergillus species

Molecular Techniques Complement Culture-Based Assessment of Bacteria Composition in Mixed Biofilms of Urinary Tract Catheter-Related Samples

Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) in reducing the motility and the biofilm formation of uropathogenic Escherichia coli

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