Impact of Prebiotics and Synbiotics Administered in ovo on the Immune Response against Experimental Antigens in Chicken Broilers

Stefaniak, Tadeusz; Madej, Janusz A.; Graczyk, Stanisław; Siwek, Maria; Łukaszewicz, Ewa; Kowalczyk, Artur; Sieńczyk, Marcin; Maiorano, Giuseppe; Bednarczyk, Marek

doi:10.3390/ani10040643

Cited by 25 publications

(27 citation statements)

References 44 publications

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“…B cells, by secreting antibodies, are the component of humoral immunity. The effects of the same prebiotics and synbiotics, with the same experimental schedule and application as in the present study, on the humoral immune response were studied by Stefaniak et al [ 22 ]. They found significantly higher serum IgG levels in SRBC-immunized 35-day-old Pre1- and Pre2 chickens than in the control group.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the chickens euthanized on the 21st day were immunized once on the 7th day and the birds euthanized on the 35th day were immunized twice, on the 7th and 21st days. The immunization scheme and the count of immunized chickens were described by Stefaniak et al [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…An innovative method of automatic in ovo injection facilitates the use of this approach under field conditions [ 15 ]. Previous studies showed that prebiotics or synbiotics applied in ovo on the 12th day of incubation may affect the development of the central and peripheral lymphoid organs in broiler chickens and may stimulate the secondary immune response, as well as modulate leukocyte maturation and reactivity [ 19 , 20 , 21 , 22 ]. Chickens treated in ovo with inulin or Bi 2 tos and then immunized with sheep red blood cells (SRBC) had significantly higher serum IgG levels than the control group [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers

Szczypka

Suszko-Pawłowska

Kuczkowski

et al. 2021

Animals

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The effects of in ovo-delivered prebiotics and synbiotics on the lymphocyte subsets of the lymphoid organs in non-immunized 7-day-old broiler chickens and in non-immunized, sheep red blood cells (SRBC)-immunized, and dextran (DEX)-immunized 21- and 35-day-old birds were studied. The substances were injected on the 12th day of egg incubation: Prebiotic1 group (Pre1) with a solution of inulin, Prebiotic2 group (Pre2) with a solution of Bi2tos (non-digestive transgalacto-oligosaccharides), Synbiotic1 group (Syn1) with inulin and Lactococcus lactis subsp. lactis IBB SL1, and Synbiotic2 group (Syn2) with Bi2tos and Lactococcus lactis subsp. cremoris IBB SC1. In 7-day-old chicks, a decrease in T splenocytes was noticed in all groups. The most pronounced effect in 21- and 35-day-old birds was an increase in TCRγδ+ cells in Syn1 and Syn2 groups. A decrease in bursal B cells was observed in DEX-immunized Pre1 group (21-day-old birds), and in the Syn1 group in non-immunized and SRBC-immunized 35-day-old birds. An increase in double-positive lymphocytes was observed in Pre1 (35-day-old birds) and Pre2 (immunized 21-day-old birds) groups. In Pre1 and Syn1 groups (21- and 35-day-old), an increase in B splenocytes and a decrease in T splenocytes were observed. We concluded that Syn1 was the most effective in the stimulation of the chicken immune system.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers

Szczypka

Suszko-Pawłowska

Kuczkowski

et al. 2021

Animals

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“…Considering the weakened state of the immune system of neonatal chickens, early establishment of gut microbiota with bene cial microbes may reduce the risk of post-hatch infection 21,24 . There is some evidence that early intestinal colonization of bene cial bacteria through in ovo inoculation or oral administration immediately post-hatch modulates innate responses as well as cell-and antibodymediated immune responses to different antigens 20,25,26 . The present study was conducted to evaluate and compare the effects of in ovo or oral administration of probiotic bacteria on innate and adaptive immune responses of newly hatched chicks.…”

Section: Discussionmentioning

confidence: 99%

In Ovo and Oral Administration of Probiotic Lactobacilli Modulate Cell- and Antibody-Mediated Immune Responses in Newly Hatched Chicks

Alizadeh

Bavananthasivam

Shojadoost

et al. 2020

Preprint

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There is some evidence that lactobacilli can strengthen immune system of chickens. This study evaluated the effects of in ovo and oral administration of a lactobacilli cocktail on cytokine gene expression, antibody-mediated immune response, and spleen cellularity in chickens. Lactobacilli were administered either in ovo at embryonic day 18, orally to hatched chicks at days 1, 7, 14, 21, and 28 post-hatches, or by both treatments combined. On days 5 and 10 post-hatch, spleen and bursa of Fabricius were collected for gene expression and cell composition analysis. On days 14 and 21 post-hatch, birds were injected with sheep red blood cells (SRBC) and keyhole limpet hemocyanin (KLH), and sera were collected on days 7, 14, and 21 post-primary immunization. Birds that received lactobacilli (107 CFU) via in ovo followed by weekly oral administration showed a greater immune response by enhancing antibody response, increasing the percentage of CD4+ and CD4+CD25+ T cells in the spleen, and upregulating the expression of the expression of interferon (IFN)-α, IFN-β, interleukin (IL)-12, and IL-13 in the spleen and expression of IFN-γ, IL-2, IL-6, IL-8, IL-12, and IL-18 in bursa. These findings suggest that pre-and post-hatch administration of lactobacilli modulate immune response in newly hatched chickens.

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“…The last, but not the least, these efforts of academia, the animal industry, and the government authorities should be in harmony to resolve these issues. A c c e p t e d A r t i c l e Inulin Increased heterophils:lymphocytes ratio [106] Colonization of CD8α + T cells and γδ + T cells in cecal tonsil [76] Inhibition of IgG retardation against SRBC [107] Yeast cell wall extract Decreased proportion of E. coli and Salmonella in cecum [108] β-glucan…”

Section: Future Perspectivementioning

confidence: 99%

Immunosecurity: immunomodulants enhance immune responses in chickens

Choi

Yun

2021

Anim Biosci

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The global population has increased with swift urbanization in developing countries, and it is likely to result in a high demand for animal-derived protein-rich foods. Animal farming has been constantly affected by various stressful conditions, which can be categorized into physical, environmental, nutritional, and biological factors. Such conditions could be exacerbated by banning on the use of antibiotics as a growth promoter together with a pandemic situation including, but not limited to, African swine fever, avian influenza, and foot-and-mouth disease. To alleviate these pervasive tension, various immunomodulants have been suggested as alternatives for antibiotics. Various studies have investigated how stressors (i.e., imbalanced nutrition, dysbiosis, and disease) could negatively affect nutritional physiology in chickens. Importantly, the immune system is critical for host protective activity against pathogens, but at the same time excessive immune responses negatively affect its productivity. Yet, comprehensive review articles addressing the impact of such stress factors on the immune system of chickens are scarce. In this review, we categorize these stressors and their effects on the immune system of chickens and attempt to provide immunomodulants which can be a solution to the aforementioned problems facing the chicken industry.

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Impact of Prebiotics and Synbiotics Administered in ovo on the Immune Response against Experimental Antigens in Chicken Broilers

Cited by 25 publications

References 44 publications

Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers

Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers

In Ovo and Oral Administration of Probiotic Lactobacilli Modulate Cell- and Antibody-Mediated Immune Responses in Newly Hatched Chicks

Immunosecurity: immunomodulants enhance immune responses in chickens

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