Impact of prepubertal exposure to dietary protocatechuic acid on the hypothalamic-pituitary-testicular axis in rats

Adedara, Isaac A.; Omole, Olubunmi; Okpara, Edozie Samuel; Fasina, Opeyemi B.; Ayeni, Modupe F.; Ajayi, Olamide M.; Busari, Esther O.; Farombi, Ebenezer O.

doi:10.1016/j.cbi.2018.05.013

Cited by 21 publications

(12 citation statements)

References 55 publications

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“…Additionally, PCA has been shown to enhance testosterone levels in male rats (Adedara et al, 2018;Beytur et al, 2012), which is consistent with our results in DSF-treated rats. Although the testosterone levels in treated rats were not closed to that of control animals, it might be enough to regulate normal spermatogenesis.…”

Section: Discussionsupporting

confidence: 92%

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

et al. 2020

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Dolichandrone serrulata flower (DSF) has been believed to reduce blood glucose in hyperglycaemic persons with sub-fertility but its effect on improvement of male reproductive impairment has never been elucidated scientifically. This study attempted to investigate the hypoglycaemic effects of DSF on male reproductive damages in type 2 diabetes mellitus (T2DM) rats. Adult Sprague Dawley rats were divided into four groups (control, T2DM, DSF200 + T2DM and DSF600 + T2DM; n = 10/each). Control rats received low-fat diet for 14 days before saline injection while streptozocin (50 mg/kg BW) induced T2DM groups received high-fat diet and were orally administered with DSF (200 and 600 mg/kg BW) for 28 days. At the end, fasted blood glucose (FBG), malondialdehyde (MDA), testosterone, sperm quality, histology and protein expressions were examined. The result showed that DSF decreased high FBG and testicular MDA and increased testosterone levels of T2DM-treated rats. Lowsperm quality and histological malfunction were ameliorated in DSF-treated group. There was significant decrease in the expression of androgen receptor, heat-shock 70 and steroidogenic acute regulatory proteins of T2DM-treated rats. Our study demonstrated changes of six bands (116, 51, 45, 39, 35 and 29 kDas) of tyrosinephosphorylated proteins. In conclusion, DSF could reduce the FBGand ameliorate the reproductive damages in male T2DM rats.

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Section: Discussionsupporting

confidence: 92%

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

et al. 2020

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“…Besides, PCA reportedly exhibits pro‐apoptotic effects in cancer cells whereas it elicits anti‐apoptotic effects in normal cells (Zambonin et al, ). Lately, chronic oral administration of PCA has been shown to be safe and boosted spermatogenic indices by inhibiting inflammation, mitigating oxidative stress and apoptosis both in normal and diabetic rats (Adedara et al, , ).…”

Section: Introductionmentioning

confidence: 99%

Protocatechuic acid inhibits testicular and epididymal toxicity associated with methotrexate in rats

et al. 2019

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We examined the effect of protocatechuic acid (PCA) on methotrexate (MTX)-induced testicular and epididymal toxicity in Wistar rats, treated with MTX (20 mg/kg) alone or in combination with PCA (25 and 50 mg/kg) body weight for a week. PCA significantly abated MTX-mediated increase in reactive oxygen and nitrogen species generation and lipid peroxidation as well as enhances glutathione balance and antioxidant enzymes in the testes and epididymis of treated animals. PCA suppressed MTX-mediated increases in interleukin-1β, tumour necrosis factor alpha and caspase-3 activity in treated animals. Additionally, PCA treatment mediated increases in luteinising and follicle-stimulating hormones, prolactin and testosterone levels with marker enzymes of testicular function, accompanied with increase in sperm functionality in treated animals. Conclusively, PCA may serve as potential supplementation, enhancing reproductive health in males undergoing MTX therapy. K E Y W O R D Sapoptosis, methotrexate, oxido-inflammation, protocatechuic acid, reproductive toxicity

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“…Thus, these data indicate that the antiapoptotic benefits of PCA may be attributed to its direct ROS scavenging activity and influencing degradation of H 2 O 2 by increasing the activity of endogenous antioxidant enzyme, which was consistent with other studies. [38][39][40] Since ROS scavenging activity partially reflects antioxidant potential, the antioxidant property of PCA may ) (E, G, H). Data are expressed as the means AE SEM (n ¼ 3).…”

Section: Discussionmentioning

confidence: 99%

Protocatechuic Acid Protects Platelets from Apoptosis via Inhibiting Oxidative Stress-Mediated PI3K/Akt/GSK3β Signaling

Hong

et al. 2021

Thromb Haemost

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Oxidative stress plays crucial roles in initiating platelet apoptosis that facilitates the progression of cardiovascular diseases (CVDs). Protocatechuic acid (PCA), a major metabolite of anthocyanin cyanidin-3-O-β-glucoside (Cy-3-g), exerts cardioprotective effects. However, underlying mechanisms responsible for such effects remain unclear. Here, we investigate the effect of PCA on platelet apoptosis and the underlying mechanisms in vitro. Isolated human platelets were treated with hydrogen peroxide (H2O2) to induce apoptosis with or without pretreatment with PCA. We found that PCA dose-dependently inhibited H2O2-induced platelet apoptosis by decreasing the dissipation of mitochondrial membrane potential, activation of caspase-9 and caspase-3, and decreasing phosphatidylserine exposure. Additionally, the distributions of Bax, Bcl-xL, and cytochrome c mediated by H2O2 in the mitochondria and the cytosol were also modulated by PCA treatment. Moreover, the inhibitory effects of PCA on platelet caspase-3 cleavage and phosphatidylserine exposure were mainly mediated by downregulating PI3K/Akt/GSK3β signaling. Furthermore, PCA dose-dependently decreased reactive oxygen species (ROS) generation and the intracellular Ca2+ concentration in platelets in response to H2O2. N-Acetyl cysteine (NAC), a ROS scavenger, markedly abolished H2O2-stimulated PI3K/Akt/GSK3β signaling, caspase-3 activation, and phosphatidylserine exposure. The combination of NAC and PCA did not show significant additive inhibitory effects on PI3K/Akt/GSK3β signaling and platelet apoptosis. Thus, our results suggest that PCA protects platelets from oxidative stress-induced apoptosis through downregulating ROS-mediated PI3K/Akt/GSK3β signaling, which may be responsible for cardioprotective roles of PCA in CVDs.

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Impact of prepubertal exposure to dietary protocatechuic acid on the hypothalamic-pituitary-testicular axis in rats

Cited by 21 publications

References 55 publications

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

Protocatechuic acid inhibits testicular and epididymal toxicity associated with methotrexate in rats

Protocatechuic Acid Protects Platelets from Apoptosis via Inhibiting Oxidative Stress-Mediated PI3K/Akt/GSK3β Signaling

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