Impact of protein supplementation on semen quality, fertility, andBMP1Rgene expression in sheep of Bangladesh

Abstract

A study was carried out to know the impact of protein supplementation of fertility and expressions of fertility gene BMP1R. Three ISO-caloric but different levels of protein supplement ration (11.70% CP for control/To, 12.99% CP for T1 and 13.86% CP for T2) was fed to three different groups of sheep. DNA was extracted from the whole blood sample for PCR of the BMP1R fertility gene and purified PCR products were sequenced by Sanger sequencer. Sequence alignment, pair and multi-alignment comparison of the BMP1R gene of the species were done with MEGA6. The semen volume (1.0 ml), sperm counts (4.2×10 7 million), percentage of normal (94.3%) and viable sperm (3.7%) were higher in treatment 2 than the other two groups. The semen volume (1.0 ml), sperm counts (4.2×107 million), the percentage of normal (94.3%) and viable sperm (3.7%) were higher in treatment 2 than the other two groups. Ewes treated with supplemented, protein concentrate reached the conception at an earlier age (Treatment 1, 9.5 ± 0.16 months and treatment 2, 10.3 ± 0.04 months) than control (9.8 ± 0.15 months). The lambing interval varied, from 198 to 202 days. Lamb’s birth weights of three treated groups were ranging from 1.2 to 1.39 kg. The designated sequences of BMP1R gene were revealed 100% homology with the sequence of Kazakh sheep. The present study indicated that the influence of nutrition on reproductive performance and genomic study will be helpful for genetic improvement of low productive sheep.