PurposeThe intersection of digital twins and ecological diversity offers a new and complex landscape that requires interdisciplinary investigation. Recognizing the urgent need for a nuanced approach to the ethical, technological and practical challenges in this area, this paper presents a conceptual framework that serves as a roadmap for future research and policymaking.Design/methodology/approachThis study employs a conceptual methodology deeply rooted in interdisciplinary perspectives. A systematic literature review was conducted using the Scopus database to identify relevant articles. The selected articles were rigorously analyzed to derive key dimensions, which were then validated through expert panel reviews and a pilot study.FindingsOur framework identifies seven critical dimensions: Data Acquisition and Simulation, Impact Assessment, Ecological Protection and Management, Ethical and Legal Considerations, Social and Cultural Impacts, Technological Feasibility and Limitations and Policies and Regulations. These dimensions provide a comprehensive structure for understanding and addressing the intersection of digital twins and biodiversity conservation.Originality/valueThis study contributes a foundational guide for sustainable and ethical engagement between digital twins and biodiversity conservation. It offers a novel, interdisciplinary framework that integrates diverse perspectives and provides practical insights for scholars, policymakers and practitioners in this emerging field.