Impact of Ridomil, Bavistin and Agrothoate on arbuscular mycorrhizal fungal colonization, biochemical changes and potassium content of cucumber plants

Rabab, A. Metwally; Reda, Enji

doi:10.1007/s10646-019-02042-0

Cited by 25 publications

(6 citation statements)

References 42 publications

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“…Thiram (2%) fungicide causes an increase in MDA content in diseased pepper plant leaves relative to control ones. This result was compatible with results of Metwally and Abdelhameed [67] in cucumber leaves treated with Ridomil. Thiram (2%) caused oxidative stress in pepper by producing ROS, which resulted in peroxidation of membranous lipids and the formation of MDA [110].…”

Section: Effect Of B Amyloliquefaciens Rash1 On Lipid Peroxidation In...supporting

confidence: 92%

“…Synthetic chemical fungicides have been a mainstay in agriculture for controlling fungal diseases for decades; however, fungicides, like other pesticides, can have negative non-target effects on the environment, such as beneficial fungi to plant growth [65]. Wide fungicide use influences the mutualist fungi such as arbuscular mycorrhizal fungi [66,67]. Our results showed that the inoculation of pepper plants with B. amyloliquefaciens RaSh1 bacterial endophyte significantly reduced the DI under A. alternata attack challenged conditions.…”

Section: B Amyloliquefaciens Rash1 Ameliorative Response To Pepper Du...mentioning

confidence: 65%

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In vivo and In vitro evaluation of the antifungal activity of the PGPR Bacillus amyloliquefaciens RaSh1 (MZ945930) against Alternaria alternata with growth promotion influences on Capsicum annuum L. plants

2023

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Alternaria alternata that threatens pepper production and causes major economic harm is responsible for the leaf spot/blight disease. Chemical fungicides have been widely employed; unfortunately, fungicidal resistance is a current concern. Therefore, finding new environmentally friendly biocontrol agents is a future challenge. One of these friendly solutions is the use of bacterial endophytes that have been identified as a source of bioactive compounds. The current study investigates the in vivo and in vitro fungicidal potential of Bacillus amyloliquefaciens RaSh1 (MZ945930) against pathogenic A. alternata. In vitro, the results revealed that RaSh1 exhibited strong antagonistic activity against A. alternata. In addition to this, we inoculated pepper (Capsicum annuum L.) plants with B. amyloliquefaciens RaSh1 and infected them with A. alternata. As a result of A. alternata infection, which generated the highest leaf spot disease incidence (DI), the plant's growth indices and physio-biochemical characteristics significantly decreased, according to our findings. Our results also showed the abnormal and deformed cell structure using light and electron microscopy of A. alternata-infected leaves compared with other treatments. However, DI was greatly reduced with B. amyloliquefaciens RaSh1 application (40%) compared to pepper plants infected with A. alternata (80%), and this led to the largest increases in all identified physio-biochemical parameters, including the activity of the defense-related enzymes. Moreover, inoculation of pepper plants with B. amyloliquefaciens RaSh1 decreased electrolyte leakage by 19.53% and MDA content by 38.60% as compared to A. alternata infected ones. Our results show that the endophyte B. amyloliquefaciens RaSh1 has excellent potential as a biocontrol agent and positively affects pepper plant growth.

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Section: Effect Of B Amyloliquefaciens Rash1 On Lipid Peroxidation In...supporting

confidence: 92%

Section: B Amyloliquefaciens Rash1 Ameliorative Response To Pepper Du...mentioning

confidence: 65%

In vivo and In vitro evaluation of the antifungal activity of the PGPR Bacillus amyloliquefaciens RaSh1 (MZ945930) against Alternaria alternata with growth promotion influences on Capsicum annuum L. plants

2023

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“…Unfortunately, these molecules may also affect soil plant-beneficial microorganisms such as AM fungi (Jin et al, 2013;Buysens et al, 2015). Their impact on these belowground microorganisms have been investigated under field (e.g., Schalamuk et al, 2014;Rivera-Becerril et al, 2017), greenhouse (e.g., Jin et al, 2013;Channabasava et al, 2015;Rabab and Reda, 2019) and growth chamber (de Novais et al, 2019a) conditions as well as in vitro on root organs (e.g., Campagnac et al, 2008;Zocco et al, 2008) or whole plants (e.g., Zocco et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Fungicides With Contrasting Mode of Action Differentially Affect Hyphal Healing Mechanism in Gigaspora sp. and Rhizophagus irregularis

et al. 2021

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Fungicides are widely used in conventional agriculture to control fungal diseases, but may also affect non-target microorganisms such as arbuscular mycorrhizal (AM) fungi. These root symbionts develop extended mycelial networks within the soil via mechanisms such as anastomosis that indistinctly concerns intact and damaged hyphae, the latter being named hyphal healing mechanism (HHM). The HHM differs between Glomeraceae and Gigasporaceae. However, the effects of fungicides on this mechanism in unknown. Here, the impact of azoxystrobin, pencycuron, flutolanil, and fenpropimorph at 0.02 and 2 mg L–1 were tested in vitro on the HHM of Gigaspora sp. MUCL 52331 and Rhizophagus irregularis MUCL 41833, and repair events visualized carefully under a dissecting bright-field light microscope. Azoxystrobin was the more detrimental for both AM fungi at 2 mg L–1, while fenpropimorph impacted only R. irregularis (stimulating at low and inhibiting at high concentration). Conversely, flutolanil and pencycuron did not impact any of the two AM fungi. The mechanisms involved remains to be elucidated, but perturbation in the still-to-be firmly demonstrated spitzenkörper or in sterols content as well as a process of hormesis are possible avenues that deserve to be explored in view of a rationale management of chemicals to control fungal pathogens without harming the beneficial AM fungi.

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“…The elucidation for these findings is that AM fungal association enhances the uptake of water and nutrients by cucumber plants through the presence of extensive extra-radical hyphal networks in soils that are more thin than roots and can pass through smaller pores; they obtain macronutrients (such as orthophosphate) and micronutrients (such as Zn 2+ ) from soil that is unobtainable to roots (Hao et al 2019 ; Metwally and Abdelhameed 2019 ; Abdelhameed and Metwally 2022 ). Also, these fungi aid in the improvement of morphological and physiological mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Attenuation of Zucchini mosaic virus disease in cucumber plants by mycorrhizal symbiosis

Metwally,

Taha,

El-Moaty

et al. 2024

Plant Cell Rep

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Key message Arbuscular mycorrhizal fungi generated systemic acquired resistance in cucumber to Zucchini yellow mosaic virus, indicating their prospective application in the soil as a sustainable, environmentally friendly approach to inhibit the spread of pathogens. Abstract The wide spread of plant pathogens affects the whole world, causing several plant diseases and threatening national food security as it disrupts the quantity and quality of economically important crops. Recently, environmentally acceptable mitigating practices have been required for sustainable agriculture, restricting the use of chemical fertilizers in agricultural areas. Herein, the biological control of Zucchini yellow mosaic virus (ZYMV) in cucumber (Cucumis sativus L.) plants using arbuscular mycorrhizal (AM) fungi was investigated. Compared to control plants, ZYMV-infected plants displayed high disease incidence (DI) and severity (DS) with various symptoms, including severe yellow mosaic, mottling and green blisters of leaves. However, AM fungal inoculation exhibited 50% inhibition for these symptoms and limited DS to 26% as compared to non-colonized ones. The detection of ZYMV by the Enzyme-Linked Immunosorbent Assay technique exhibited a significant reduction in AM-inoculated plants (5.23-fold) compared with non-colonized ones. Besides, mycorrhizal root colonization (F%) was slightly reduced by ZYMV infection. ZYMV infection decreased all growth parameters and pigment fractions and increased the malondialdehyde (MDA) content, however, these parameters were significantly enhanced and the MDA content was decreased by AM fungal colonization. Also, the protein, proline and antioxidant enzymes (POX and CAT) were increased with ZYMV infection with more enhancements due to AM root colonization. Remarkably, defence pathogenesis-related (PR) genes such as PR-a, PR-b, and PR-10 were quickly expressed in response to AM treatment. Our findings demonstrated the beneficial function of AM fungi in triggering the plant defence against ZYMV as they caused systemic acquired resistance in cucumber plants and supported their potential use in the soil as an environment-friendly method of hindering the spread of pathogenic microorganisms sustainably.

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Impact of Ridomil, Bavistin and Agrothoate on arbuscular mycorrhizal fungal colonization, biochemical changes and potassium content of cucumber plants

Cited by 25 publications

References 42 publications

In vivo and In vitro evaluation of the antifungal activity of the PGPR Bacillus amyloliquefaciens RaSh1 (MZ945930) against Alternaria alternata with growth promotion influences on Capsicum annuum L. plants

In vivo and In vitro evaluation of the antifungal activity of the PGPR Bacillus amyloliquefaciens RaSh1 (MZ945930) against Alternaria alternata with growth promotion influences on Capsicum annuum L. plants

Fungicides With Contrasting Mode of Action Differentially Affect Hyphal Healing Mechanism in Gigaspora sp. and Rhizophagus irregularis

Attenuation of Zucchini mosaic virus disease in cucumber plants by mycorrhizal symbiosis

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