Impact of root-associated strains of three Paraburkholderia species on primary and secondary metabolism of Brassica oleracea

Jeon, Je-Seung; Carreño-Quintero, Natalia; Eekelen, Henriëtte D. L. M. van; Vos, Ric C. H. de; Raaijmakers, Jos M.; Etalo, Desalegn W.

doi:10.1038/s41598-021-82238-9

Cited by 26 publications

(22 citation statements)

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“…CCGE1002 from Mimosa occidentalis ) or bacteria found in the root endosphere ( P. hospita BT03 was found in Populus deltoides ) ( Gottel et al, 2011 ; Ormeño-Orrillo et al, 2012 ; Taulé et al, 2012 ). P. hospita mHSR1 is a root-associated strain, which induces a systemic resistance against the leaf pathogen Xanthomonas campestris in Brassica oleracea , and Variovorax PMC12 promotes growth of Solanum lycopersicum under abiotic stress conditions as well as induces resistance to wilt disease caused by Ralstonia solanacearum ( Lee et al, 2018 ; Jeon et al, 2021 ). Interestingly, downstream of P. phymatum VgrG (Bphy_5985) within the T6SS-b cluster, where usually effectors are located, we found two genes coding for potential sodium:dicarboxylate symporters (Bphy_5990 and Bphy_5992; Figure 1A ).…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

et al. 2021

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Paraburkholderia phymatum STM815, a rhizobial strain of the Burkholderiaceae family, is able to nodulate a broad range of legumes including the agriculturally important Phaseolus vulgaris (common bean). P. phymatum harbors two type VI Secretion Systems (T6SS-b and T6SS-3) in its genome that contribute to its high interbacterial competitiveness in vitro and in infecting the roots of several legumes. In this study, we show that P. phymatum T6SS-b is found in the genomes of several soil-dwelling plant symbionts and that its expression is induced by the presence of citrate and is higher at 20/28°C compared to 37°C. Conversely, T6SS-3 shows homologies to T6SS clusters found in several pathogenic Burkholderia strains, is more prominently expressed with succinate during stationary phase and at 37°C. In addition, T6SS-b expression was activated in the presence of germinated seeds as well as in P. vulgaris and Mimosa pudica root nodules. Phenotypic analysis of selected deletion mutant strains suggested a role of T6SS-b in motility but not at later stages of the interaction with legumes. In contrast, the T6SS-3 mutant was not affected in any of the free-living and symbiotic phenotypes examined. Thus, P. phymatum T6SS-b is potentially important for the early infection step in the symbiosis with legumes.

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Section: Discussionmentioning

confidence: 99%

Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

et al. 2021

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“…Another candidate probiotic, P. fungorum , is a plant probiotic that can increase the growth and production of strawberries. P. fungorum is a novel microbiome-based plant breeding strategy that can regulate growth and disease defense [ 28 ]. It is known that E. ludwigii is a candidate probiotic in aquaculture, as it enhances the digestive enzyme activity of Haliotis asinina L. In addition, E. ludwigii isolated from Aedes albopictus has been shown to inhibit La Crosse encephalitis, suggesting that the bacteria may secrete antiviral metabolites [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Administration of Novel Multistrain Probiotics from Healthy Grouper Intestines Promotes the Intestinal Immune Response against NNV Infection

Joe

Sung

et al. 2021

Life

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Epinephelus lanceolatus (giant grouper) is a high-value cultured species in the Asia-Pacific region. However, nervous necrosis virus (NNV) is an infectious viral disease that affects over 120 species of marine cultured species and causes high mortality, ranging from 90–100% in the grouper industry. Probiotics isolated from the intestines of healthy individuals have provided insight into novel approaches involved in the defense against viral pathogens. In this study, we isolated three strains of bacteria as candidate probiotics from healthy grouper intestines and a 28-day feeding trial was performed. At day 21, the nervous necrosis virus (NNV) challenge test was conducted for 7 days to evaluate the antiviral effect of candidate probiotics. The results showed that candidate probiotics could improve growth conditions, such as weight gain (WG) and specific growth rate (SGR), and increase the utilization of feed. Furthermore, the candidate probiotic mixture had the ability to protect against NNV, which could decrease the mortality rate by 100% in giant grouper after NNV challenge. Subsequently, we analyzed the mechanism of the candidate probiotic mixture’s defense against NNV. A volcano plot revealed 203 (control vs. NNV), 126 (NNV vs. probiotics–NNV), and 5 (control vs. probiotics–NNV) differentially expressed transcripts in intestinal tissue. Moreover, principal components analysis (PCA) and cluster analysis heatmap showed large differences among the three groups. Functional pathway analysis showed that the candidate probiotic mixture could induce the innate and adaptive immunity of the host to defend against virus pathogens. Therefore, we hope that potential candidate probiotics could be successfully applied to the industry to achieve sustainable aquaculture.

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“…Extracts were analyzed by accurate mass LC-MS as described previously by Jeon et al ( 2021 ) with minor adaptations. An UltiMate 3000 U-HPLC system (Dionex, Sunnyvale, CA, USA) was used to create a 45 min linear gradient of 5–75% acetonitrile in a 0.1% FA water a flow rate of 0.19 mL min −1 .…”

Section: Methodsmentioning

confidence: 99%

Elicitor Application in Strawberry Results in Long-Term Increase of Plant Resilience Without Yield Loss

et al. 2021

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For a first step integrating elicitor applications into the current IPM strategy increasing plant resilience against pests, we investigated repeated elicitor treatments in a strawberry everbearer nursery and cropping cycle under glass. During nursery methyl-jasmonate (MeJA), testing induction of defenses with plant bioassays was applied every 3 weeks. Thrips damage and reproduction by spider mites, whitefly and aphids were strongly reduced upon elicitor treatment. Subsequently, we applied MeJA every 3 weeks or based on scouting pests during a whole cropping cycle. Thrips leaf bioassays and LC-MS leaf metabolomics were applied to investigate the induction of defenses. Leaf damage by thrips was lower for both MeJA application schemes compared to the control except for the last weeks. While elicitor treatments after scouting also reduced damage, its effect did not last. Thrips damage decreased from vegetative to mature plants during the cropping cycle. At the end of the nursery phase, plants in the elicitor treatment were smaller. Surprisingly, growth during production was not affected by MeJA application, as were fruit yield and quality. LC-MS leaf metabolomics showed strong induction of vegetative plants decreasing during the maturation of plants toward the end of cultivation. Concurrently, no increase in the JA-inducible marker PPO was observed when measured toward the end of cultivation. Mostly flavonoid and phenolic glycosides known as plant defense compounds were induced upon MeJA application. While induced defense decreased with the maturation of plants, constitutive defense increased as measured in the leaf metabolome of control plants. Our data propose that young, relatively small plant stages lack constitutive defense necessitating an active JA defense response. As plants, mature constitutive defense metabolites seem to accumulate, providing a higher level of basal resistance. Our results have important implications for but are not limited to strawberry cultivation. We demonstrated that repeated elicitor application could be deployed as part of an integrated approach for sustainable crop protection by vertical integration with other management tactics and horizontal integration to control multiple pests concurrently. This approach forms a promising potential for long-term crop protection in greenhouses.

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Impact of root-associated strains of three Paraburkholderia species on primary and secondary metabolism of Brassica oleracea

Cited by 26 publications

References 58 publications

Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

Dietary Administration of Novel Multistrain Probiotics from Healthy Grouper Intestines Promotes the Intestinal Immune Response against NNV Infection

Elicitor Application in Strawberry Results in Long-Term Increase of Plant Resilience Without Yield Loss

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