Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation

Khalil, Wael A.; El-Harairy, M.; Zeidan, A. E. B.; Hassan, Mahmoud A. E.

doi:10.1016/j.theriogenology.2018.12.017

Cited by 101 publications

(127 citation statements)

References 45 publications

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“…The free radical scavenging function of zinc is well established through earlier in vivo and in vitro studies [38,86]. Moreover, it enhanced the post-thawing quality when supplemented to rooster semen extender [28,47] as well as protected the spermatozoa quality (motility, DNA integrity) and spermatogenesis against oxidative damage when administered orally [48]. Therefore, it is probable that the protection of membrane peroxidation by ZnONPs and SeNPs through the reduction of MDA and increasing the SOD and GSH help in mitigating the subsequent damaging effect on the macromolecules like DNA and crucial organelles like mitochondria [38,87,88].…”

Section: Discussionmentioning

confidence: 97%

“…Safa et al [28] showed that the addition of 5 mg/mL of vitamin E to freezing diluents combined with 1% SeNPs improved the post-thawing sperm motility, vitality, and oxidative variables in rooster semen. Khalil et al [47] recommended an increase in the supplementation concentration of SeNPs from 0.5 to 1.0 mg/mL to Tris-egg yolk extenders after observing an increase in the percentage of viable sperm, and a decrease in early apoptotic, apoptotic, and necrotic sperm in post-thawed bull semen. However, increasing the concentration of SeNPs to 1.5 mg/mL adversely affected the viable sperm and increased the amount of apoptotic and necrotic sperm.…”

Section: Discussionmentioning

confidence: 99%

“…The extender was composed of 74 mL buffer + 6 mL glycerol + 20 mL egg yolk. The following supplements were added to the SHOTER extender to examine the effects on spermatozoa cryopreservation: Vit E (α tocopherol, T3251) at 200 µM/mL [55], Vit C (ascorbic acid, A4544) at 1 mg/mL [18], SeNPs at 1 µg/mL [47], ZnONPs at 50 µg/mL [20], Na 2 SeO 3 (Loba Chemie Pvt. Ltd., Mumbai, India) at 2 µg/mL [32], and ZnSO 4 (Loba Chemie) at 100 µM/mL [19].…”

Section: Experimental Designmentioning

confidence: 99%

“…Several studies have used nano-selenium (SeNPs) as a ROS scavenger to safeguard against oxidative damage in sperm cells. Adding SeNPs to the semen extender enhanced the post-thawing quality and oxidizing rooster semen variables [28,47]. The oral supplementation of SeNPs also protected the spermatozoa quality (motility, DNA integrity) and spermatogenesis against oxidative damage caused by cisplatin, a male reproductive toxicant [48].…”

Section: Introductionmentioning

confidence: 96%

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Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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There are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. Epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. In the current study, we compared the effects of supplementation with vitamin C, E, inorganic trace elements of selenium (Na2SeO3) and zinc (ZnSO4), and zinc and selenium nanoparticles (ZnONPs and SeNPs, respectively) on the cryopreservation of dromedary camel epididymal spermatozoa. When the SHOTOR extender was supplemented with ZnONPs and SeNPs; the sperm showed increased progressive motility; vitality; and membrane integrity after cooling at 5 °C for 2 h; when compared to the control and vitamin-supplemented groups. Moreover, the ZnONPs and SeNPs supplementation improved the progressive motility, vitality, sperm membrane integrity, ultrastructural morphology, and decreased apoptosis when frozen and thawed. SeNPs significantly increased reduced glutathione (GSH), superoxide dismutase (SOD), and decreased lipid peroxide malondialdehyde (MDA) levels. The advantageous effects of the trace elements were potentiated by reduction into a nano-sized particle, which could increase bioavailability and reduce the undesired liberation of toxic concentrations. We recommend the inclusion of SeNPs or ZnONPs to SHOTOR extenders to improve the cryotolerance of camel epididymal spermatozoa.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

Self Cite

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“…The former includes superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx) and catalase (CAT), while the latter includes reduced glutathione (GSH), vitamins, plant extracts (e.g., cinnamtannin B-1), minerals, amino acids, proteins and other exogenous compounds (e.g., resveratrol or quercetin) with antioxidant properties [123,139]. Recent studies investigated the addition of different nanoparticles to the freezing medium to overcome the main drawbacks that conventional antioxidants could present, like the low durability to harsh conditions [140][141][142][143]. Nanotechnology advances have contributed to the design of novel nano-compounds that possess antioxidant properties, such as selenium, zinc oxide and apoferritin containing gold-silver nanoparticles.…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Mechanisms, Applications, and Challenges of Utilizing Nanomaterials in Cryopreservation

Wang,

Gao,

Shu

2024

Adv Eng Mater

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Cryopreservation of biological samples, including cells, tissues, and organs, has become an essential component in various biomedical research and applications, such as cellular therapy, tissue engineering, organ transplantation, and conservation of endangered species. However, it faces critical challenges throughout the cryopreservation process, such as loading/unloading of cryoprotective agent (CPA), ice inhibition during cooling, and ultrafast and uniform heating during rewarming. Applying nanomaterials in cryopreservation has emerged as a promising solution to address these challenges in each step due to their unique properties. For instance, in order to deliver nonpermeating CPA into cells, some nanomaterials, such as polymeric nanocapsule, can carry nonpermeating CPA to penetrate into the cells, regulating the intracellular ice crystal. During cooling, some nanomaterials, such as graphene oxide, can bind to basal or prism planes of ice crystals, suppressing the ice growth. During rewarming, some nanomaterials, such as magnetic nanoparticles, can improve the heating performance, preventing devitrification and recrystallization during rewarming. However, challenges in nanomaterials‐assisted cryopreservation remain, including the need for comprehensive studies on nanomaterials toxicity and the development of scalable manufacturing processes for industrial applications. This review examines the role of nanomaterials in cryopreservation, focusing on their mechanisms, applications, and associated challenges.

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Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation

Cited by 101 publications

References 45 publications

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Mechanisms, Applications, and Challenges of Utilizing Nanomaterials in Cryopreservation

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