Impact of serum and dialysates obtained from chronic hemodialysis patients maintained on high cut‐off membranes on inflammation profile in human THP‐1 monocytes

Trojanowicz, Bogusz; Ulrich, Christof; Fiedler, Roman; Storr, Markus; Boehler, Torsten; Martus, Peter; Pawlak, Michael; Glomb, Marcus A.; Henning, Christian; Templin, Markus F.; Werner, Kristin; Zickler, Daniel; Willy, Kevin; Schindler, Ralf; Girndt, Matthias

doi:10.1111/hdi.12494

Cited by 7 publications

(5 citation statements)

References 44 publications

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“…Several trials have meanwhile provided evidence suggesting that larger pore membranes indeed enhance clearance of different middle molecules, and can reduce inflammation and calcification. A study in hemodialysis patients treated with HCO vs. high-flux membranes demonstrated by exposing THP-1 monocytes their serum or to the dialysate that HCO membranes eliminate a spectrum of pro-inflammatory mediators from serum into the dialysate [ 41 ]. In patients with signs of chronic mild inflammation (C-reactive protein (CRP) > 5 mg/l), use of HCO in series with a low-flux dialyzer resulted in a dampening of systemic inflammation markers such as soluble TNF receptor 1 (sTNFR1), associated with (cardiovascular) outcomes in observational CKD cohorts [ 42 ], but not of expression density of the P-selectin receptor CD162 on monocytes in pre-dialysis blood samples [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the association between increasing membrane pore size and endotoxin permeability using a novel experimental dialysis simulation set-up

et al. 2018

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Background: Membranes with increasing pore size are introduced to enhance removal of large uremic toxins with regular hemodialysis. These membranes might theoretically have higher permeability for bacterial degradation products. In this paper, permeability for bacterial degradation products of membranes of comparable composition with different pore size was investigated with a new in vitro set-up that represents clinical flow and pressure conditions. Methods: Dialysis was simulated with an AK200 machine using a low-flux, high-flux, medium cut-off (MCO) or high cut-off (HCO) device (n = 6/type). A polyvinylpyrrolidone-solution (PVP) was recirculated at blood side. At dialysate side, a challenge solution containing a filtrated lysate of two water-borne bacteria (Pseudomonas aeruginosa and Pelomononas saccharophila) was infused in the dialysate flow (endotoxin ≥ 4EU/ml). Blood and dialysate flow were set at 400 and 500 ml/min for 60 min. PVP was sampled before (PVP pre ) and after (PVP post ) the experiment and dialysate after 5 and 55 min. Limulus Amebocyte Lysate (LAL) test was performed. Additionally, samples were incubated with a THP-1 cell line (24 h) and IL-1β levels were measured evaluating biological activity.

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Section: Discussionmentioning

confidence: 99%

Assessment of the association between increasing membrane pore size and endotoxin permeability using a novel experimental dialysis simulation set-up

et al. 2018

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“…The effects of different membrane characteristics could then be evaluated without influences from sequestration. Studies comparing the use of high cut-off versus high-flux dialysis membranes on the cytokine production profile [109] and the angiotensin-system [110] found less inflammation and a modification of the ACE/ACE2 relation on monocytes towards the normal situation. This might be taken as a hint that the alterations in dialysis patients may be improved by dialysis technology that removes middle molecules (15-45 kD molecular weight).…”

Section: The Role Of Hemodialysis Treatment On Monocytes Phenotype and Functionmentioning

confidence: 99%

Monocytes in Uremia

Girndt

Trojanowicz

Ulrich

2020

Toxins

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Monocytes play an important role in both innate immunity and antigen presentation for specific cellular immune defense. In patients with chronic renal failure, as well as those treated with maintenance hemodialysis, these cells are largely dysregulated. There is a large body of literature on monocyte alterations in such patients. However, most of the publications report on small series, there is a vast spectrum of different methods and the heterogeneity of the data prevents any meta-analytic approach. Thus, a narrative review was performed to describe the current knowledge. Monocytes from patients with chronic renal failure differ from those of healthy individuals in the pattern of surface molecule expression, cytokine and mediator production, and function. If these findings can be summarized at all, they might be subsumed as showing chronic inflammation in resting cells together with limited activation upon immunologic challenge. The picture is complicated by the fact that monocytes fall into morphologically and functionally different populations and population shifts interact heavily with dysregulation of the individual cells. Severe complications of chronic renal failure such as impaired immune defense, inflammation, and atherosclerosis can be related to several aspects of monocyte dysfunction. Therefore, this review aims to provide an overview about the impairment and activation of monocytes by uremia and the resulting clinical consequences for renal failure patients.

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“…In particular, HCO dialysis cleared β2-microglobulin, sTNF-RI, factor D, and high molecular AGEs better than HF membranes did [200]. The recent report in which exposure to HCO serum markedly lowered the levels of 72 pro-inflammatory transcripts in THP1 cells (including TNF-α and IL-6) compared with exposure to HF serum demonstrated that HCO dialyzers may also directly decrease the inflammatory potential of monocytes and the subsequent calcification of vascular/valvular cells [201]. Indeed, VSMC calcification was 43% lower when the cells were incubated in vitro with HCO dialysis serum, relative to HF dialysis serum; this effect was accompanied by a 26% relative decrease in ALP expression [202].…”

Section: The Impact Of Ckd On Macrophage Functions: Consequences Fmentioning

confidence: 99%

New Insights into the Roles of Monocytes/Macrophages in Cardiovascular Calcification Associated with Chronic Kidney Disease

Hénaut¹,

Candellier

Boudot³

et al. 2019

Toxins

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Cardiovascular disease (CVD) is an important cause of death in patients with chronic kidney disease (CKD), and cardiovascular calcification (CVC) is one of the strongest predictors of CVD in this population. Cardiovascular calcification results from complex cellular interactions involving the endothelium, vascular/valvular cells (i.e., vascular smooth muscle cells, valvular interstitial cells and resident fibroblasts), and monocyte-derived macrophages. Indeed, the production of pro-inflammatory cytokines and oxidative stress by monocyte-derived macrophages is responsible for the osteogenic transformation and mineralization of vascular/valvular cells. However, monocytes/macrophages show the ability to modify their phenotype, and consequently their functions, when facing environmental modifications. This plasticity complicates efforts to understand the pathogenesis of CVC—particularly in a CKD setting, where both uraemic toxins and CKD treatment may affect monocyte/macrophage functions and thereby influence CVC. Here, we review (i) the mechanisms by which each monocyte/macrophage subset either promotes or prevents CVC, and (ii) how both uraemic toxins and CKD therapies might affect these monocyte/macrophage functions.

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Impact of serum and dialysates obtained from chronic hemodialysis patients maintained on high cut‐off membranes on inflammation profile in human THP‐1 monocytes

Abstract: Taken together, these data demonstrate that High cut-off -membranes eliminate a spectrum of mediators from serum into the dialysate that possess proinflammatory properties and may impair cellular viability.

Cited by 7 publications

References 44 publications

Assessment of the association between increasing membrane pore size and endotoxin permeability using a novel experimental dialysis simulation set-up

Assessment of the association between increasing membrane pore size and endotoxin permeability using a novel experimental dialysis simulation set-up

Monocytes in Uremia

New Insights into the Roles of Monocytes/Macrophages in Cardiovascular Calcification Associated with Chronic Kidney Disease

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