2020
DOI: 10.1016/j.mee.2020.111405
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Impact of serum concentration in cell culture media on tight junction proteins within a multiorgan microphysiological system

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Cited by 25 publications
(25 citation statements)
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“…It was due to the ample supply of FBS within the microphysiological system by cell culture media. 10% FBS in cell culture media results in continuously increasing TEER values and tighter cell–cell tight junction formation compared to serum-free or serum reduced cell culture media [ 38 ]. TGF-β1 in the concentration of 5 ng/mL was used as a stimulant to induce fibrosis in the liver fibrosis-on-chip model [ 39 ].…”
Section: Resultsmentioning
confidence: 99%
“…It was due to the ample supply of FBS within the microphysiological system by cell culture media. 10% FBS in cell culture media results in continuously increasing TEER values and tighter cell–cell tight junction formation compared to serum-free or serum reduced cell culture media [ 38 ]. TGF-β1 in the concentration of 5 ng/mL was used as a stimulant to induce fibrosis in the liver fibrosis-on-chip model [ 39 ].…”
Section: Resultsmentioning
confidence: 99%
“…The level of relevance of a microphysiological system (MPS) with human physiology makes them ideal for identifying the novel therapeutics. The organ-on-a-chip and spheroid culture systems are evolving to better mimic human pathophysiology, which is being enhanced by the integration of sensors and robotics [1][2][3][4][5][6][7][8]. Among MPS, spheroids are emerging with the potential to mimic human tissues in a 3D shape and function [9][10][11].…”
Section: Introductionmentioning
confidence: 99%
“…A previously validated TEER sensor was used as an additional parameter for assessing cell layer confluency in a chip platform [ 8 , 10 , 25 , 26 ]. The TEER sensor was used to assess the real-time effect of different ECMs on the barrier integrity of HepG2 cell monolayer formed in the MPS based on impedance monitoring for 144 h ( Figure 4 and Figure S9 ).…”
Section: Resultsmentioning
confidence: 99%
“…It was observed that ECM has a significant impact on the formation of tight junctions. TEER values among different ECMs have a substantial effect on the MPS-based real-time biological assays, as several researchers have employed TEER for estimating cell viability, fibrosis development, and FBS standardization [ 8 , 27 , 28 , 29 ]. It can be concluded that the choice of ECM is vital for developing the most physiologically relevant MPSs.…”
Section: Resultsmentioning
confidence: 99%
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