Impact of silk fibroin-based scaffold structures on human osteoblast MG63 cell attachment and&amp;nbsp;proliferation

A, Varkey; Venugopal, Elakkiya; Sugumaran, Pon Janani; Janarthanan, Gopinathan; Pillai, Mamatha M.; Selvakumar, R.; Bhattacharyya, Amitava

doi:10.2147/ijn.s82209

Cited by 17 publications

(12 citation statements)

References 29 publications

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“…The percentage of SF (n%) within the prepared SF/CMCS hydrogel was measured based on the Bradford protein assay [69] using the following equation:…”

Section: Evaluation Of Sf Content In Sf/cmcs Hydrogelmentioning

confidence: 99%

“…The prepared SF/CMCS hydrogel was placed in a weighing bottle containing 20 mL (V) of deionized water at 25 • C with a relative humidity of 75%. At different time intervals (t = 10 min, 30 min, 1 h, 3 h, and 6 h), the content of SF in the deionized water was measured via the Bradford protein assay [69], recorded as C. Then, the SF/CMCS hydrogels were lyophilized and weighed, which was recorded as m. The percentage of SF released from the SF/CMCS hydrogel (Q%) can be calculated using the following formula, in which n% is the percentage of SF in the SF/CMCS hydrogel, determined from Formula (3): The samples were first freeze-dried using a lyophilizer (LGJ-10, Shanghai YuMing Instrument Co., Ltd., Shanghai, China). Subsequently, the lyophilized samples were sputtercoated (GSL-1100X-SPC-16m, MTI Corporation, Richmond, VA, USA) with gold to give them a conductive coating.…”

Section: Quantifying the Sf Releasementioning

confidence: 99%

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Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing

Chen

Zhang

Jiang

et al. 2021

IJMS

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At present, silk fibroin (SF) hydrogel can be prepared by means of electrodeposition at 25 V in direct current (DC) mode. Reducing the applied voltage would provide benefits, including lower fabrication costs, less risk of high voltage shocks, and better stability of devices. Here, a simple but uncommon strategy for SF-based hydrogel preparation using 4 V in DC mode is discussed. SF was mixed and cross-linked with carboxymethyl chitosan (CMCS) through hydrogen bonding, then co-deposited on the graphite electrode. The thickness, mass, and shape of the SF/CMCS hydrogel were easily controlled by adjusting the electrodeposition parameters. Morphological characterization of the prepared hydrogel via SEM revealed a porous network within the fabricated hydrogel. This structure was due to intermolecular hydrogen bonding between SF and CMCS, according to the results of thermogravimetric analysis and rheological measurements. As a potential wound dressing, SF/CMCS hydrogel maintained a suitable moisture environment for wound healing and demonstrated distinct properties in terms of promoting the proliferation of HEK-293 cells and antibacterial activity against Escherichia coli and Staphylococcus aureus. Furthermore, histological studies were conducted on a full-thickness skin wound in rats covered with the SF/CMCS hydrogel, with results indicating that this hydrogel can promote wound re-epithelization and enhance granulation tissue formation. These results illustrate the feasibility of using the developed strategy for SF-based hydrogel fabrication in practice for wound dressing.

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“…The percentage of SF (n%) within the prepared SF/CMCS hydrogel was measured based on the Bradford protein assay [69] using the following equation:…”

Section: Evaluation Of Sf Content In Sf/cmcs Hydrogelmentioning

confidence: 99%

Section: Quantifying the Sf Releasementioning

confidence: 99%

Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing

Chen

Zhang

Jiang

et al. 2021

IJMS

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“…The method was modified from the literature [31]. After 3 days of cell seeding into the scaffolds, the cellseeded scaffolds were gently washed with PBS.…”

Section: Fluorescent Stainingmentioning

confidence: 99%

Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane

2021

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Angiogenesis is a crucial step in tissue regeneration and repair. Biomaterials that allow or promote angiogenesis are thus beneficial. In this study, angiogenic properties of salt-leached silk fibroin (SF) scaffolds seeded with human adipose stem cells (hADSCs) were studied using chick chorioallantoic membrane (CAM) as a model. The hADSC-seeded SF scaffolds (SF-hADSC) with the porosity of 77.34 ± 6.96% and the pore diameter of 513.95 ± 4.99 µm were implanted on the CAM of chick embryos that were on an embryonic day 8 (E8) of development. The SF-hADSC scaffolds induced a spoke-wheel pattern of capillary network indicative of angiogenesis, which was evident since E11. Moreover, the ingrowth of blood vessels into the scaffolds was seen in histological sections. The unseeded scaffolds induced the same extent of angiogenesis later on E14. By contrast, the control group could not induce the same extent of angiogenesis. In vitro cytotoxicity tests and in vivo angioirritative study reaffirmed the biocompatibility of the scaffolds. This work highlighted that the biocompatible SF-hADSC scaffolds accelerate angiogenesis, and hence they can be a promising biomaterial for the regeneration of tissues that require angiogenesis.

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“…Other materials that have been used in combination with SF to create scaffolds include agarose [66], type II collagen (col II) [67], type I collagen (col I) [68], and hydroxyapatite (Hap) [69]. The biocompatibility of SF has also been demonstrated in applications associated with the repair of bone [70][71][72][73][74][75][76][77], meniscus [78], skin [79], and tendon defects [3].…”

Section: Biocompatibilitymentioning

confidence: 99%

Scaffolds Based on Silk Fibroin for Osteochondral Tissue Engineering

Moncada‐Saucedo

Camacho‐Morales

Fuentes-Mera

2019

RDMS

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Physical and chemical features of silk fibroin as a biomaterialSFs from the cocoons of the silkworm B. mori are the most-used and most-studied SFs in TE; South Korea is home to over 300 varieties of this species [16]. Silkworm cocoons primarily consist of 2 bio-macromolecules: fibroin (fibrous protein) and sericin (globular protein). The silk of B. mori is synthesized in a group of specialized salivary glands, and fibroin (comprising 60-80% of the silk) is synthesized exclusively in the posterior region of the gland. The fibroin fibres are covered with sericin (15-35%), which is synthesized in the walls of the medial regions of the gland. Between 1 and 5% of the silk consists of non-sericin components, such as pigments, wax, sugars, and other impurities (Figure 1), [1,17,18].Most lepidopterans produce fibroin that consists of 3 protein components arranged as a single element of silk: heavy-chain (~391kDa) and light-chain fibroins (~25kDa), which are linked by a disulphide bridge, and P25 (~25kDa), also known as fibrohexamerin (Fhx), These components are present at a 6:6:1 ratio, respectively [19][20][21].

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Impact of silk fibroin-based scaffold structures on human osteoblast MG63 cell attachment and proliferation

Cited by 17 publications

References 29 publications

Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing

Preparation of Silk Fibroin/Carboxymethyl Chitosan Hydrogel under Low Voltage as a Wound Dressing

Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane

Scaffolds Based on Silk Fibroin for Osteochondral Tissue Engineering

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