Impact of sleep restriction in B-1 cells activation and differentiation

Vidal, Andrey Sladkevicius; Reis, Natasha Ferraz de Campos; Lorenzo, Beatriz Helena Pizarro De; Alvares-Saraiva, Anuska Marcelino; Xander, Patrícia; Brito, Rosineide Santana de

doi:10.1016/j.imbio.2022.152280

Cited by 3 publications

(11 citation statements)

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“…In that study, sleep restriction alone was able to increase the population of B-1 cells producing NO and ROS, polarize the cell towards a myeloid profile, suppress the gene expression of some TLRs as well as the expression of the IL-10 gene [19]. Additionally, this study also demonstrated that intraperitoneal stimulation of C57BL/6J mice with L. amazonensis promastigotes led to different effects on B-1 cells of sleep-restricted mice compared to non-restricted controls [19]. Thus, sleep restriction altered the response pattern of B-1 cells in sleep-restricted animals infected with the parasite compared to those infected without sleep restriction.…”

Section: Discussionmentioning

confidence: 92%

“…However, the physiology of these cells under stress conditions still needs to be addressed. In previous study, our group showed that stress induced by sleep restriction for 21 days (SR21) led to changes in the behavior of B-1 cells [19]. In that study, sleep restriction alone was able to increase the population of B-1 cells producing NO and ROS, polarize the cell towards a myeloid profile, suppress the gene expression of some TLRs as well as the expression of the IL-10 gene [19].…”

Section: Discussionmentioning

confidence: 93%

“…In previous study, our group showed that stress induced by sleep restriction for 21 days (SR21) led to changes in the behavior of B-1 cells [19]. In that study, sleep restriction alone was able to increase the population of B-1 cells producing NO and ROS, polarize the cell towards a myeloid profile, suppress the gene expression of some TLRs as well as the expression of the IL-10 gene [19]. Additionally, this study also demonstrated that intraperitoneal stimulation of C57BL/6J mice with L. amazonensis promastigotes led to different effects on B-1 cells of sleep-restricted mice compared to non-restricted controls [19].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, our group demonstrated that sleep restriction over 21 days prompted the differentiation of peritoneal B-1 cells into B-1 cellderived phagocytes (B-1 CDP), enhancing their microbicidal capacity through increased production of nitric oxide (NO) and reactive oxygen species (ROS). This shift was accompanied by a harmful modulation of lymphoid commitment factors in B-1 cells from the sleep-restricted group [19]. A hypothesis regarding the potential influence of sleep on the immune system is supported by observations that sleep restriction can impact several immune cells and their functions, such as phagocytosis [20], antigen uptake [21], antibody production [22,23], and the levels of autoantibodies [24].…”

Section: Introductionmentioning

confidence: 95%

“…In certain experimental contexts or specific conditions, B-1 cells may be associated with myeloid or lymphoid lineage commitment. [12,19]. Thus, we investigated the expression of genes related to lymphoid and myeloid lineages in B-1 cells from C57BL/6J mice subjected to sleep restriction and intraperitoneally stimulated with P. brasiliensis or C. albicans fungi.…”

Section: Expression Of Lymphoid and Myeloid Commitment Genes In B-1 C...mentioning

confidence: 99%

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Sleep Restriction Modulates B‐1 Cell Response Following Fungal Infection

Vidal,

Alvares-Saraiva,

Segura

et al. 2024

Preprint

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B-1 cells, a subtype of B lymphocytes, are found in lymphoid tissues and peritoneal and pleural cavities. They play diverse roles, releasing cytokines, generating antibodies, regulating the immune response, engaging in phagocytosis, and moving to inflammatory sites. However, their physiology remains partially understood, especially in stressful conditions like sleep restriction. This study aimed to investigate murine B-1 cell activation and differentiation under sleep restriction. Male C57BL/6J mice underwent an 18-hour sleep restriction for 21 days, followed by Candida albicans or Paracoccidioides brasiliensis infection. Purified peritoneal B-1 cells were evaluated for activation markers, microbicidal molecules, cytokine gene expression, Toll-like receptors, and commitment factors. Results showed that sleep restriction minimally influenced activation marker expression in B-1 cells across infection models. However, under sleep restriction, microbicidal molecule production increased in P. brasiliensis (NO) and C. albicans (ROS) infections. Gene expression revealed elevated TLR-2 and cytokines (IL-12, IL-6, TNF-α) in sleep-restricted animals. Lineage commitment analysis indicated myeloid polarization in sleep-restricted B-1 cells for all infection models. These findings underscore the impact of sleep restriction on B-1 cell activation and differentiation, with outcomes varying based on infection type. This study highlights the importance of investigating immunological changes resulting from sleep restriction.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Expression Of Lymphoid and Myeloid Commitment Genes In B-1 C...mentioning

confidence: 99%

See 3 more Smart Citations

Sleep Restriction Modulates B‐1 Cell Response Following Fungal Infection

Vidal,

Alvares-Saraiva,

Segura

et al. 2024

Preprint

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Extracellular vesicles and sleep deprivation

Novaes e Brito

2024

Current Topics in Membranes

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Analysis of the correlation between immune cell characteristics and insomnia: a Mendelian randomization study

Han,

Song,

et al. 2024

Journal of Neurophysiology

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Background: Insomnia, recognized as a prevalent sleep disorder, has garnered extensive attention within the realm of public health. Recent studies indicate a close interaction between the immune system and sleep, however, the specific mechanism remains not yet fully understood.Methods: This study employed a comprehensive two-sample Mendelian randomization analysis to elucidate the correlation between immune cell characteristics and insomnia. Utilizing publicly available genetic data, we investigated the causal relationship between 731 immune cell traits and insomnia risk. Comprehensive sensitivity analyses were conducted to verify the robustness, heterogeneity, and horizontal pleiotropy of the results.Results: In this study, we identified that 14 immune phenotypes among four immune profiles (MFI, RC, AC, and MP) demonstrated a significant causal association with insomnia. Specifically, eight immune cell phenotypes were associated with an increased risk of insomnia, including CD11c+ monocyte% (P<0.001), CD11c+ HLA DR++ monocyte% (P =0.004), CD86+ plasmoid dendritic cell (DC) AC (P <0.001), CD33br HLA DR+ CD14dim AC (P <0.001), CD8dim AC (P =0.002), CCR2 on CD14+ CD16- monocyte (P <0.001), CD39 on monocyte (P<0.001), and SSC-A on myeloid DC (P<0.001). Six immune cell phenotypes demonstrated protective effects against insomnia, including PB/PC% B cell (P<0.001), CM CD4+% CD4+ (P<0.001), T cell AC (P<0.001), BAFF-R on IgD- CD38br (P<0.001), CD16-CD56 on HLA DR+ NK cells (P<0.001), and CD14 on CD33br HLA DR+ CD14dim (P<0.001).Conclusions: Our study established the correlation between immune cells and insomnia via the Mendelian randomization method, offering a novel theoretical foundation for the concept of sleep-immune crosstalk.

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Impact of sleep restriction in B-1 cells activation and differentiation

Cited by 3 publications

References 51 publications

Sleep Restriction Modulates B‐1 Cell Response Following Fungal Infection

Sleep Restriction Modulates B‐1 Cell Response Following Fungal Infection

Extracellular vesicles and sleep deprivation

Analysis of the correlation between immune cell characteristics and insomnia: a Mendelian randomization study

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