Impact of SMOFLipid on Pulmonary Alveolar Development in Newborn Guinea Pigs

Lavoie, Jean-Claude; Mohamed, Ibrahim; Nuyt, Anne‐Monique; Elremaly, Wesam; Rouleau, Thérèse

doi:10.1002/jpen.1153

Cited by 16 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, Lavoie et al . described the increased oxidative stress in the lung tissue of newborn guinea pigs administered ω-3 PUFA (SMOFlipid) compared with ω-6 PUFA (Intralipid) 43 . In our study, we did not find the elevated MDA content, the marker of lipoperoxidation, in any group.…”

Section: Discussionmentioning

confidence: 99%

The effect of ω-3 polyunsaturated fatty acids on the liver lipidome, proteome and bile acid profile: parenteral versus enteral administration

Bechyňská

Ďásková

Vrzáčková

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Parenteral nutrition (PN) is often associated with the deterioration of liver functions (PNALD). Omega-3 polyunsaturated fatty acids (PUFA) were reported to alleviate PNALD but the underlying mechanisms have not been fully unraveled yet. Using omics´ approach, we determined serum and liver lipidome, liver proteome, and liver bile acid profile as well as markers of inflammation and oxidative stress in rats administered either ω-6 PUFA based lipid emulsion (Intralipid) or ω-6/ω-3 PUFA blend (Intralipid/Omegaven) via the enteral or parenteral route. In general, we found that enteral administration of both lipid emulsions has less impact on the liver than the parenteral route. Compared with parenterally administered Intralipid, PN administration of ω-3 PUFA was associated with 1. increased content of eicosapentaenoic (EPA)- and docosahexaenoic (DHA) acids-containing lipid species; 2. higher abundance of CYP4A isoenzymes capable of bioactive lipid synthesis and the increased content of their potential products (oxidized EPA and DHA); 3. downregulation of enzymes involved CYP450 drug metabolism what may represent an adaptive mechanism counteracting the potential negative effects (enhanced ROS production) of PUFA metabolism; 4. normalized anti-oxidative capacity and 5. physiological BAs spectrum. All these findings may contribute to the explanation of ω-3 PUFA protective effects in the context of PN.

show abstract

Section: Discussionmentioning

confidence: 99%

The effect of ω-3 polyunsaturated fatty acids on the liver lipidome, proteome and bile acid profile: parenteral versus enteral administration

Bechyňská

Ďásková

Vrzáčková

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In animal studies, two authors confirmed the anti-oxidative effect of FO in guinea pigs [18] or rats subjected to intestinal ischemia [19]. In contrast, Lavoie et al described the increased oxidative stress in the lung tissue of guinea pigs administered FO (SMOFlipid) compared with SO (Intralipid) [20].…”

Section: Introductionmentioning

confidence: 94%

The ω-3 Polyunsaturated Fatty Acids and Oxidative Stress in Long-Term Parenteral Nutrition Dependent Adult Patients: Functional Lipidomics Approach

et al. 2020

View full text Add to dashboard Cite

Omega-3 polyunsaturated fatty acids (ω-3PUFAs) are introduced into parenteral nutrition (PN) as hepatoprotective but may be susceptible to the lipid peroxidation while olive oil (OO) is declared more peroxidation resistant. We aimed to estimate how the lipid composition of PN mixture affects plasma and erythrocyte lipidome and the propensity of oxidative stress. A cross-sectional comparative study was performed in a cohort of adult patients who were long-term parenterally administered ω-3 PUFAs without (FO/–, n = 9) or with (FO/OO, n = 13) olive oil and healthy age- and sex-matched controls, (n = 30). Lipoperoxidation assessed as plasma and erythrocyte malondialdehyde content was increased in both FO/– and FO/OO groups but protein oxidative stress (protein carbonyls in plasma) and low redox status (GSH/GSSG in erythrocytes) was detected only in the FO/– subcohort. The lipidome of all subjects receiving ω-3 PUFAs was enriched with lipid species containing ω-3 PUFAs (FO/–˃FO/OO). Common characteristic of all PN-dependent patients was high content of fatty acyl-esters of hydroxy-fatty acids (FAHFAs) in plasma while acylcarnitines and ceramides were enriched in erythrocytes. Plasma and erythrocyte concentrations of plasmanyls and plasmalogens (endogenous antioxidants) were decreased in both patient groups with a significantly more pronounced effect in FO/–. We confirmed the protective effect of OO in PN mixtures containing ω-3 PUFAs.

show abstract

“…The approach used to obtain a gradation of the redox potential in vivo was to feed the animal with a different mode of nutrition, oral (ON) versus parenteral (PN), to vary the quality of lipid emulsion of these PNs (higher level of omega-6 fatty acids in Intralipid than in SMOFLipid), and protect or not these PNs from ambient light (which promotes the generation of peroxides). The oxidative nature of the PN [ 19 ], particularly if the PN contained SMOFLipid instead of Intralipid has already been reported in the lungs of guinea pigs [ 15 ]. Although exposure to light affected hepatic vitamin C levels, it had no influence on the redox potential.…”

Section: Discussionmentioning

confidence: 99%

“…Animals on PN were nourished through a catheter inserted in the jugular vein under anaesthesia with a ketamine/xylazine cocktail on the third day of life, as previously described [ 9 , 15 , 16 ]. PN solutions, freshly prepared daily, consisted of two separate bags.…”

Section: Methodsmentioning

confidence: 99%

“…This mode of nutrition induces an oxidative stress caused by the contamination of PN with oxidant molecules such as H 2 O 2 [ 13 ], lipid peroxides [ 13 , 14 ] and aldehydes [ 14 ]. PN induces an oxidative shift of the glutathione redox potential in the lungs [ 15 ] and livers of animals [ 16 ], and in the blood of premature infants [ 17 , 18 ]. Hydrogen peroxide is the result of the reduction of dissolved oxygen by vitamin C present in parenteral solution, i.e., a reaction by which vitamin C is oxidized into dehydroascorbate that is rapidly hydrolysed into diketogulonate [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between redox potential of glutathione and DNA methylation level in liver of newborn guinea pigs

et al. 2020

Self Cite

View full text Add to dashboard Cite

The metabolism of DNA methylation is reported to be sensitive to oxidant molecules or oxidative stress. Hypothesis: early-life oxidative stress characterized by the redox potential of glutathione influences the DNA methylation level. The in vivo study aimed at the impact of modulating redox potential of glutathione on DNA methylation. Newborn guinea pigs received different nutritive modalities for 4 days: oral nutrition, parenteral nutrition including lipid emulsion Intralipid (PN-IL) or SMOFLipid (PN-SF), protected or not from ambient light. Livers were collected for biochemical determinations. Redox potential (p < 0.001) and DNA methylation (p < 0.01) were higher in PN-infused animals and even higher in PN-SF. Their positive correlation was significant (r 2 = 0.51; p < 0.001). Methylation activity was higher in PN groups (p < 0.01). Protein levels of DNA methyltransferase (DNMT)-1 were lower in PN groups (p < 0.01) while those of both DNMT3a isoforms were increased (p < 0.01) and significantly correlated with redox potential (r 2 > 0.42; p < 0.001). The ratio of SAM (substrate) to SAH (inhibitor) was positively correlated with the redox potential (r 2 = 0.36; p < 0.001). In conclusion, early in life, the redox potential value strongly influences the DNA methylation metabolism, resulting in an increase of DNA methylation as a function of increased oxidative stress. These results support the notion that early-life oxidative stress can reprogram the metabolism epigenetically. This study emphasizes once again the importance of improving the quality of parenteral nutrition solutions administered early in life, especially to newborn infants. Abbreviation of Title: Parenteral nutrition and DNA methylation

show abstract

Impact of SMOFLipid on Pulmonary Alveolar Development in Newborn Guinea Pigs

Abstract: In our model, SMOF is pro-oxidant and induces hypo-alveolarization following exaggerated apoptosis. These results highlight the need for further studies before introducing SMOFLipid in standard neonatal care.

Cited by 16 publications

References 31 publications

The effect of ω-3 polyunsaturated fatty acids on the liver lipidome, proteome and bile acid profile: parenteral versus enteral administration

The effect of ω-3 polyunsaturated fatty acids on the liver lipidome, proteome and bile acid profile: parenteral versus enteral administration

The ω-3 Polyunsaturated Fatty Acids and Oxidative Stress in Long-Term Parenteral Nutrition Dependent Adult Patients: Functional Lipidomics Approach

Relationship between redox potential of glutathione and DNA methylation level in liver of newborn guinea pigs

Contact Info

Product

Resources

About