Impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes

Esbert, Marga; Pacheco, A.; Vidal, Francesca; Florensa, M.; Riqueros, M.; Ballesteros, A.; Garrido, Nicolás; Calderón, Gloria

doi:10.1016/j.rbmo.2011.07.010

Cited by 80 publications

(50 citation statements)

References 37 publications

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“…Though the relationship between sperm assessment and accurately diagnosing male infertility or predicting conception remains ambiguous [2,[21][22][23], semen analysis is the first diagnostic tool used to determine a contributing male factor in an infertile couple. Manual semen analysis, based largely on the evaluation of sperm concentration, motility and morphology, is known to suffer from lack of standardization, repeatability and accuracy [4].…”

Section: Discussionmentioning

confidence: 99%

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Lammers¹,

Splingart

Barrière

et al. 2013

J Assist Reprod Genet

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Purpose Despite controversy regarding its clinical value, male fertility investigation mainly relies on semen analysis. Even though reference guidelines are available, manual sperm analysis still suffers from analytical variability, thus questioning the interest of automated sperm analysis systems. The aim of this study is to compared automated computerized semen analysis systems (SQA-V GOLD and CASA CEROS) to the conventional manual method in terms of accuracy and precision. Methods We included 250 men in this double-blind prospective study. The SQA-V GOLD (Medical Electronic Systems) and CEROS, CASA system (Hamilton Thorne) were compared to the standard manual assessment based on the WHO 5th Edition. The main outcome measures were sperm concentration, total sperm number, total motility, progressive motility, non-progressive motility, morphology, motile sperm concentration (MSC) and progressively motile sperm concentration (PMSC) with the three methods. Results Statistical analysis of the test results from the automated systems and the manual method demonstrated no significant differences for most of the semen parameters. The Spearman coefficients of rank correlation (rho) for CASA and the SQA-V GOLD automated systems vs. the manual method were: Sperm concentration (0.95 and 0.95), total sperm number (0.95 and 0.95), MSC (0.94 and 0.96) and PMSC (0.94 and 0.93) correspondingly. Concerning sperm morphology, both automated systems demonstrated high specificity (Sp) and negative predictive values (NPV), despite significantly different medians (CASA: 83.7 % for Sp and 95.2 % for NPV, SQA-V: 97.9 % for Sp and 92.5 %). The highest precision (lowest 95 % confidence interval for duplicate tests) for all semen variables was found in the SQA-V GOLD. Conclusions The advantages of using automated semen analysers are: Standardization, speed (lower turnaround time), precision, reduced potential for human error, automated data recording and less need for highly skilled professionals to run the systems. The disadvantages of using automated systems are: notably the problem with testing some atypical samples and the inability to perform an assessment of morphology abnormalities. Based on the results of this study, the SQA-V Gold demonstrated better agreement vs. the manual method. In conclusion, automated semen analyzers can be used for routine semen analysis providing rapid clinically acceptable results with higher precision, and positively impacting laboratory standardization.

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Section: Discussionmentioning

confidence: 99%

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Lammers¹,

Splingart

Barrière

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

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“…Several studies have shown that the prevalence of males with high levels of SDF is greater in the infertile than in the fertile population (Spano et al, 2000;Zini et al, 2002), and that many couples with unexplained infertility have higher levels of SDF (Host et al, 1999;Oleszczuk et al, 2013). However, studies examining the effect of high SDF on assisted conception fertilization, embryo development, pregnancy, miscarriage, and birth outcomes report conflicting results; some studies report a correlation between SDF and outcomes for both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles (Collins et al, 2008;Robinson et al, 2012), others that IVF but not ICSI outcomes are influenced (Bungum et al, 2007;Zini, 2011;Osman et al, 2015), and others have found no clear association with either assisted fertilization method (Esbert et al, 2011;Ni et al, 2014). These inconsistencies may in part be attributed to study design and the use of different assays, with a recent study reporting that Comet and TUNEL assays were more predictive of clinical outcomes than SCSA (Simon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Bradley¹,

McArthur²,

Gee³

et al. 2016

Andrology

105

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SUMMARYSperm DNA fragmentation (SDF) is used in assisted reproductive technology (ART) programs as an indicator for sperm quality, although there is still a lack of consensus as to its clinical utility. In this retrospective study, we examined intracytoplasmic sperm injection (ICSI) outcomes of 1924 infertile patients who underwent SDF analysis using the sperm chromatin integrity test. ART patients were classified as having low [DNA fragmentation index (DFI) <29%] or high SDF (DFI ≥29%) and by whether or not an intervention [physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection (IMSI), testicular sperm extraction (TESE)/testicular sperm aspiration (TESA), frequent ejaculation] was performed. High SDF patients who did not have an intervention had a lower fertilization rate and poorer clinical outcomes from blastocyst transfers as compared with low SDF patients; the fertilization rate was 66.0% vs. 70.2% (p = 0.042), single embryo transfer (SET) fetal heart pregnancy rate was 28.5% vs. 45.2% (p = 0.042), and SET live birth rate was 24.9% vs. 40.6% (p = 0.060), respectively. Furthermore, high SDF patients who had an intervention had significantly improved blastocyst transfer outcomes, similar to those of low SDF patients; the SET live birth rate for high SDF intervention patients was 43.8% as compared with 24.9% for high SDF no intervention patients (p = 0.037) and 40.6% for low SDF patients (p = 0.446). Analysis of the three main intervention subgroups for high SDF patients revealed that TESE/TESA patients had the highest SET live birth rate; in comparison with 24.2% for high SDF patients who did not have an intervention, PICSI patients had 38.3% (p = 0.151), IMSI patients had 28.7% (p = 0.680), and TESE/TESA patients had 49.8% (p = 0.020). Our data suggest that SDF results indicate ICSI outcomes and that patients who have high SDF benefit from an intervention.

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“…We found a very few number of study assessing the contrary. For example in [26] study, there were no correlation between DNA fragmentation and the issue of ART technique. But it is important to note the fact that in that study, the cases included oocyte donor (so young selected women with high quality oocyte).…”

Section: Discussionmentioning

confidence: 79%

Varicocele and DNA Fragmentation in Senegalese Infertile Males—A Study of 95 Cases

Diallo¹,

Faye²,

Diallo³

et al. 2017

ARSci

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Varicocele is the first etiology of infertility in men worldwide and affects almost half of the men dealing with infertility in Senegal. Varicocele is associated with a high rate of sperm DNA fragmentation so this test is being more and more recommended especially when patients have to benefit from an ART (Assisted Reproductive Technology) procedure. The aim of our study was to assess if our patients with varicocele are concerned by higher DNA fragmentation rate in spermatozoon. Sperm samples were collected (95 cases of varicocele and 30 controls) and analyzed between January 2013 and June 2016 in a prospective study. Sperm DNA damage, characterized by the rate of DNA fragmentation was evaluated by TUNEL assay using fluorescence microscopy. There was a significant higher percentage of DNA fragmentation in patients with varicocele (p < 0.001), compared to controls. We plan to study a bigger cohort to assess this tendency and study the benefits of surgery in varicocele by evaluating DNA fragmentation after the procedure.

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Impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes

Cited by 80 publications

References 37 publications

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Varicocele and DNA Fragmentation in Senegalese Infertile Males—A Study of 95 Cases

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