Impact of surface grafting density of PEG macromolecules on dually fluorescent silica nanoparticles used for the in vivo imaging of subcutaneous tumors

Adumeau, Laurent; Genevois, Coralie; Roudier, Lydia; Schatz, Christophe; Couillaud, Franck; Mornet, Stéphane

doi:10.1016/j.bbagen.2017.01.036

Cited by 41 publications

(39 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PEG density of the SNCs ( fig. S4 and table S1) was around 0.9 molecules/nm 2 (Table 1), which is sufficient for a "brush" conformation that allows effective immune evasion (19). The surface FA density of FA-PEGmodified SNCs was around 0.09 molecules/nm 2 .…”

Section: Nonspecific and Receptor-mediated Cell Binding And Uptakementioning

confidence: 92%

Nanoparticle elasticity regulates phagocytosis and cancer cell uptake

et al. 2020

View full text Add to dashboard Cite

The ability of cells to sense external mechanical cues is essential for their adaptation to the surrounding microenvironment. However, how nanoparticle mechanical properties affect cell-nanoparticle interactions remains largely unknown. Here, we synthesized a library of silica nanocapsules (SNCs) with a wide range of elasticity (Young’s modulus ranging from 560 kPa to 1.18 GPa), demonstrating the impact of SNC elasticity on SNC interactions with cells. Transmission electron microscopy revealed that the stiff SNCs remained spherical during cellular uptake. The soft SNCs, however, were deformed by forces originating from the specific ligand-receptor interaction and membrane wrapping, which reduced their cellular binding and endocytosis rate. This work demonstrates the crucial role of the elasticity of nanoparticles in modulating their macrophage uptake and receptor-mediated cancer cell uptake, which may shed light on the design of drug delivery vectors with higher efficiency.

show abstract

Section: Nonspecific and Receptor-mediated Cell Binding And Uptakementioning

confidence: 92%

Nanoparticle elasticity regulates phagocytosis and cancer cell uptake

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Polyethylene glycol is a linker molecule frequently used to functionalise NPs for drug delivery applications (Allen and Cullis 2013) and is rationalised by the strategy of improving the circulation half-life of NPs in vivo to exploit the enhanced permeability and retention (EPR) effect for localising in tumour sites (He et al 2008). Controlling the density of PEG on the NP surfaces to form 'brush-like' conformations appears to be a crucial factor that determines the ability of NPs to evade immune cell uptake and to impart 'stealth' properties (Adumeau et al 2017;Yang et al 2014).…”

Section: Bioconjugation Strategiesmentioning

confidence: 99%

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

et al. 2020

View full text Add to dashboard Cite

Background: Fluorescent silica nanoparticles have been extensively utilised in a broad range of biological applications and are facilitated by their predictable, wellunderstood, flexible chemistry and apparent biocompatibility. The ability to couple various siloxane precursors with fluorescent dyes and to be subsequently incorporated into silica nanoparticles has made it possible to engineer these fluorophores-doped nanomaterials to specific optical requirements in biological experimentation. Consequently, this class of nanomaterial has been used in applications across immunodiagnostics, drug delivery and human-trial bioimaging in cancer research. Main body: This review summarises the state-of-the-art of the use of dye-doped silica nanoparticles in bioapplications and firstly accounts for the common nanoparticle synthesis methods, surface modification approaches and different bioconjugation strategies employed to generate biomolecule-coated nanoparticles. The use of dye-doped silica nanoparticles in immunoassays/biosensing, bioimaging and drug delivery is then provided and possible future directions in the field are highlighted. Other non-cancerrelated applications involving silica nanoparticles are also briefly discussed. Importantly, the impact of how the protein corona has changed our understanding of NP interactions with biological systems is described, as well as demonstrations of its capacity to be favourably manipulated. Conclusions: Dye-doped silica nanoparticles have found success in the immunodiagnostics domain and have also shown promise as bioimaging agents in human clinical trials. Their use in cancer delivery has been restricted to murine models, as has been the case for the vast majority of nanomaterials intended for cancer therapy. This is hampered by the need for more human-like disease models and the lack of standardisation towards assessing nanoparticle toxicity. However, developments in the manipulation of the protein corona have improved the understanding of fundamental bio-nano interactions, and will undoubtedly assist in the translation of silica nanoparticles for disease treatment to the clinic.

show abstract

“…Additionally, nanoparticles help to stabilize hydrophobic components in aqueous media and prevent degradation and inactivation of active compounds [ 15 , 16 , 17 , 18 ]. In cancer diagnosis and treatment, targeted nanoparticles can be designed and synthesized to enhance their selective uptake and retention inside tumoral cells [ 4 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…A part of using fluorescent dyes to label nanoparticles, the external surface of nanoparticles can be further functionalized with molecules that confer stability in aqueous solution. Polyethylene glycol (PEG) is a molecule that improves water stability, minimizes non-specific interactions with other molecules in the extracellular matrix, and does not activate the immune response [ 26 , 27 , 52 , 53 , 54 , 55 ]. Thus, PEG ensures nanoparticles dispersion and high bioavailability to cells.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells

Prieto-Montero

Katsumiti

Cajaraville

et al. 2020

Sensors

View full text Add to dashboard Cite

Functionalized fluorescent silica nanoparticles were designed and synthesized to selectively target cancer cells for bioimaging analysis. The synthesis method and characterization of functionalized fluorescent silica nanoparticles (50–60 nm), as well as internalization and subcellular localization in HeLa cells is reported here. The dye, rhodamine 101 (R101) was physically embedded during the sol–gel synthesis. The dye loading was optimized by varying the synthesis conditions (temperature and dye concentration added to the gel) and by the use of different organotriethoxysilanes as a second silica precursor. Additionally, R101, was also covalently bound to the functionalized external surface of the silica nanoparticles. The quantum yields of the dye-doped silica nanoparticles range from 0.25 to 0.50 and demonstrated an enhanced brightness of 230–260 fold respect to the free dye in solution. The shell of the nanoparticles was further decorated with PEG of 2000 Da and folic acid (FA) to ensure good stability in water and to enhance selectivity to cancer cells, respectively. In vitro assays with HeLa cells showed that fluorescent nanoparticles were internalized by cells accumulating exclusively into lysosomes. Quantitative analysis showed a significantly higher accumulation of FA functionalized fluorescent silica nanoparticles compared to nanoparticles without FA, proving that the former may represent good candidates for targeting cancer cells.

show abstract

Impact of surface grafting density of PEG macromolecules on dually fluorescent silica nanoparticles used for the in vivo imaging of subcutaneous tumors

Abstract: Control of the physicochemical features of nanoparticle surfaces to improve blood circulation times and monitoring of the EPR effect. This article is part of a Special Issue entitled "Recent Advances in Bionanomaterials" Guest Editor: Dr. Marie-Louise Saboungi and Dr. Samuel D. Bader.

Cited by 41 publications

References 50 publications

Nanoparticle elasticity regulates phagocytosis and cancer cell uptake

Nanoparticle elasticity regulates phagocytosis and cancer cell uptake

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells

Contact Info

Product

Resources

About