Impact of Surface Modification on Cellular Uptake and Cytotoxicity of Silica Nanoparticles

Vranic, Sandra; Watanabe, Eri; Miyakawa, Kayoko; Takeuchi, Sakie; Osada, Y.; Ichihara, Sahoko; Zong, Cai; Wu, Wenting; Sakurai, ﻿Toshihiro; Sato, Akira; Hara, Yasushi; Suzuki, Toshihiro; Ryo, Akihide; Boland, Sonja; Tran, Lang; Ichihara, Gaku

doi:10.21203/rs.3.rs-96205/v1

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Further, in the absence of FBS, like in this study, CPS20 particles, followed by CPS200, PPS20 and AMI200 particles, showed the highest rate of macrophage uptake. The preference of carboxyl-functionalized over amino-functionalized particles by macrophages appears to be independent of the particle material because it was reported for 100 nm polystyrene particles and for 25 nm silica particles [29][30][31].…”

Section: Discussionmentioning

confidence: 90%

Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

et al. 2021

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Respiratory exposure of humans to environmental and therapeutic nanoparticles repeatedly occurs at relatively low concentrations. To identify adverse effects of particle accumulation under realistic conditions, monocultures of Calu-3 and A549 cells and co-cultures of A549 and THP-1 macrophages in the air–liquid interphase culture were exposed repeatedly to 2 µg/cm2 20 nm and 200 nm polystyrene particles with different functionalization. Particle accumulation, transepithelial electrical resistance, dextran (3–70 kDa) uptake and proinflammatory cytokine secretion were determined over 28 days. Calu-3 cells showed constant particle uptake without any change in barrier function and cytokine release. A549 cells preferentially ingested amino- and not-functionalized particles combined with decreased endocytosis. Cytokine release was transiently increased upon exposure to all particles. Carboxyl-functionalized demonstrated higher uptake and higher cytokine release than the other particles in the A549/THP-1 co-cultures. The evaluated respiratory cells and co-cultures ingested different amounts and types of particles and caused small (partly transient) effects. The data suggest that the healthy cells can adapt to low doses of non-cytotoxic particles.

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Section: Discussionmentioning

confidence: 90%

Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

et al. 2021

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Development of Cell-Derived Plasma Membrane Vesicles as a Nanoparticle Encapsulation and Delivery System

Kheradmandi,

Farnoud,

Burdick

2023

Preprint

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BackgroundDeveloping non-invasive delivery platforms with a high level of structural and/or functional similarity to biological membranes is highly desirable to reduce toxicity and improve targeting capacity of nanoparticles. Numerous studies have investigated the impacts of physicochemical properties of engineered biomimetic nanoparticles on their interaction with cells, yet technical difficulties have led to the search for better biomimetics, including vesicles isolated directly from live cells. Cell-derived giant plasma membrane vesicles (GPMVs), in particular, offer a close approximation of the intact cell plasma membrane by maintaining the latter′s compositional complexity, protein positioning in a fluid-mosaic pattern, and physical and mechanical properties. Thus, to overcome technical barriers of prior nanoparticle delivery approaches, we aimed to develop a novel method using GPMVs to encapsulate a variety of engineered nanoparticles, then use these core-shell, nanoparticle-GPMV vesicle structures to deliver cargo to other cells.ResultsThe GPMV system in this study was generated by chemically inducing vesiculation in A549 cells, a model human alveolar epithelial line. These cell-derived GPMVs retained encapsulated silica nanoparticles (50 nm diameter) for at least 48 hours at 37 °C. GPMVs showed nearly identical lipid and protein membrane profiles as the parental cell plasma membrane, with or without encapsulation of nanoparticles. Notably, GPMVs were readily endocytosed in the parental A549 cell line as well as the human monocytic THP-1 cell line. Higher cellular uptake levels were observed for GPMV-encapsulated nanoparticles compared to control groups, including free nanoparticles. Further, GPMVs delivered a variety of nanoparticles to parental cells with reduced cytotoxicity compared to free nanoparticles at concentrations that were otherwise significantly toxic.ConclusionsWe have introduced a novel technique to load nanoparticles within the cell plasma membrane during the GPMV vesiculation process. These GPMVs are capable of (a) encapsulating different types of nanoparticles (including larger and not highly-positively charged bodies that have been technically challenging cargoes) using a parental cell uptake technique, and (b) improving delivery of nanoparticles to cells without significant cytotoxicity. Ultimately, endogenous surface membrane proteins and lipids can optimize the physicochemical properties of cell membrane-derived vesicles, which could lead to highly effective cell membrane-based nanoparticle/drug delivery systems.

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Cationic Phenosafranin Photosensitizers Based on Polyhedral Oligomeric Silsesquioxanes for Inactivation of Gram-Positive and Gram-Negative Bacteria

Rózga-Wijas

Bąk-Sypień

Turecka

et al. 2021

IJMS

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The high photodynamic effect of the Newman strain of the S. aureus and of clinical strains of S. aureus MRSA 12673 and E. coli 12519 are observed for new cationic light-activated phenosafranin polyhedral oligomeric silsesquioxane (POSS) conjugates in vitro. Killing of bacteria was achieved at low concentrations of silsesquioxanes (0.38 µM) after light irradiation (λem. max = 522 nm, 10.6 mW/cm2) for 5 min. Water-soluble POSS-photosensitizers are synthesized by chemically coupling a phenosafranin dye (PSF) (3,7-diamino-5-phenylphenazine chloride) to an inorganic silsesquioxane cage activated by attachment of succinic anhydride rings. The chemical structure of conjugates is confirmed by 1H, 13C NMR, HRMS, IR, fluorescence spectroscopy and UV-VIS analyzes. The APDI and daunorubicin (DAU) synergy is investigated for POSSPSFDAU conjugates. Confocal microscopy experiments indicate a site of intracellular accumulation of the POSSPSF, whereas iBuPOSSPSF and POSSPSFDAU accumulate in the cell wall or cell membrane. Results from the TEM study show ruptured S. aureus cells with leaking cytosolic mass and distorted cells of E. coli. Bacterial cells are eradicated by ROS produced upon irradiation of the covalent conjugates that can kill the bacteria by destruction of cellular membranes, intracellular proteins and DNA through the oxidative damage of bacteria.

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Impact of Surface Modification on Cellular Uptake and Cytotoxicity of Silica Nanoparticles

Cited by 3 publications

References 30 publications

Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

Development of Cell-Derived Plasma Membrane Vesicles as a Nanoparticle Encapsulation and Delivery System

Cationic Phenosafranin Photosensitizers Based on Polyhedral Oligomeric Silsesquioxanes for Inactivation of Gram-Positive and Gram-Negative Bacteria

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