2017
DOI: 10.3762/bjnano.8.10
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Impact of surface wettability on S-layer recrystallization: a real-time characterization by QCM-D

Abstract: Quartz crystal microbalance with dissipation monitoring (QCM-D) has been employed to study the assembly and recrystallization kinetics of isolated SbpA bacterial surface proteins onto silicon dioxide substrates of different surface wettability. Surface modification by UV/ozone oxidation or by vapor deposition of 1H,1H,2H,2H-perfluorododecyltrichlorosilane yielded hydrophilic or hydrophobic samples, respectively. Time evolution of frequency and dissipation factors, either individually or combined as the so-call… Show more

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Cited by 13 publications
(19 citation statements)
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“…>85% of the total mass is bound). Such an observation resembles to what has been already described by our group for stopped-flow conditions and same type of underlying substrates [19,22,24]. However, as main difference, the much larger flow rate employed in those stopped-flow experiments (300 µL/min) allowed for a faster completion of the protein adsorption (see also Supplementary Materials , Figure S1) if compared to current experiments (25 µL/min).…”
Section: Resultssupporting
confidence: 86%
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“…>85% of the total mass is bound). Such an observation resembles to what has been already described by our group for stopped-flow conditions and same type of underlying substrates [19,22,24]. However, as main difference, the much larger flow rate employed in those stopped-flow experiments (300 µL/min) allowed for a faster completion of the protein adsorption (see also Supplementary Materials , Figure S1) if compared to current experiments (25 µL/min).…”
Section: Resultssupporting
confidence: 86%
“…The assembly of a SbpA protein S-layer onto fluorinated hydrophobic SiO 2 surfaces under continuous flow conditions was initially followed by means of QCMD. This technique allows a quantitative real-time monitoring of the process in terms of bound protein mass as well as film viscous properties, according to the variations recorded for frequency (∆f ) and dissipation (∆D) factors, respectively [19]. Figure 1 depicts the results obtained for the injection of five SbpA concentrations: 100, 50, 25, 10, and 5 µg/mL.…”
Section: Resultsmentioning
confidence: 99%
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“…14,15 This is the case of SbpA from Lysinibacillus sphaericus CCM2177, which presents a square (p4) lattice symmetry, a system that has quite extensively been characterized in literature so far. [16][17][18] Because of the large number of nanometric pores found along the S-layer structure, it can be hypothesized that the release of the drug from the polymeric matrix can be still conducted although to a lower rate.…”
Section: Introductionmentioning
confidence: 99%