In recent years, the display information of head-up displays for vehicles has gradually developed from single-depth to multi-depth. To reduce the workload of driving and the number of eye adjustments, researchers use the visual perception of human eyes to realize the image information integrated with the real world. In this study, HoloLens2 is used to demonstrate head-up displays of different depths. An electroencephalogram, an electro-ophthalmogram, and a NASA-TLX questionnaire were used to evaluate the fatigue of drivers during long-term driving. The results showed that a dual-depth head-up display could effectively reduce the driver’s workload.