BackgroundThe present research was conducted to investigate the impact of reishi mushroom extract (RME) on the formation of heterocyclic aromatic amines (HAAs) in meatballs. Sample preparations involved applying RME using either the spreading or addition method, with varying concentrations (0%, 0.25%, 0.5%, and 1% of RME), followed by deep‐frying at temperatures of 150°C and 190°C for 3 minutes.ResultsThe types and levels of HAAs varied based on the frying temperature, method of extract application, and the extract concentration. Notably, total HAA contents increased with rising the frying temperature (p<0.01) and varied from undetectable levels to 4.91 ng g−1 across all analysed meatballs. The addition method was more effective than the spreading method (p<0.01), and among the concentrations tested, 0.25% RME exhibited the highest efficacy in reducing total HAAs (p<0.05). Furthermore, the addition method has inhibited lipid oxidation more efficiently compared to the surface spreading method (p<0.05).ConclusionThis study demonstrated that RME had mitigating effects on HAAs depending on the concentration and frying conditions in deep‐fried meatball samples.This article is protected by copyright. All rights reserved.