The effect of fungal fertilizers with isolates of T. harzianum and G. mosseae and levels of phosphate rock on NPK content in the barley plant was investigated. A field trial was conducted on clay loamy soil to produce a variety of barley crops named (Samir 1) during the autumn agricultural season in 2021 in the Al-Qadisiyah Governorate. The factorial experiment was designed according to a Randomized Complete Block Design (RCBD) with three replications. The factors of the experiment included two levels of T. harzianum inoculum (T0, control and T1, fungal inoculation), two levels of G. mosseae inoculum (G0, control and G1 fungal inoculation), and four levels of phosphate rock (P0, P1, P2, and P3) with an amount (0,1000,1500, and 2000) t/ha-1. The results present that the interaction treatment (G. mosseae + T. harzianum and phosphate rock of 1000 t/ha-1) resulted in a significant increase in the content of nitrogen, phosphate, and potassium (NPK) in the plant 1.977, 0.437, and 2.603 %, respectively, compared to the control.