Impact of the antioxidant quercetin on morphological integrity and follicular development in the in vitro culture of Bos indicus female ovarian fragments

Cerezetti, Marcela Bortoletto; Gonzalez, S. M.; Ferraz, Camila R.; Verri, Waldiceu Aparecido; Rabelo, Evelyn Andrade; Seneda, Marcelo Marcondes; Morotti, Fábio

doi:10.1007/s11626-021-00629-8

Cited by 10 publications

(5 citation statements)

References 37 publications

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“…Furthermore, the antioxidant capacity by the FRAP method of the medium that received 0.4 mg/ml of Moringa oleifera extract was approximately double compared to the control medium of this work, which corroborates with in vitro studies have demonstrated that these compounds can prevent damage in ovarian cells because of their cytoprotective and antioxidant properties. Cerezetti et al (2021), using 25 µg/ml of quercetin in the culture medium of bovine ovarian fragments, found a greater capacity to reduce ferric ions by the FRAP method when compared to the control.…”

Section: Ferric Reducing Antioxidant Power (Frap)mentioning

confidence: 87%

“…In bovine granulosa cells, quercetin reduced the response to oxidative stress mediated by the Nrf2 regulator at the transcriptional and post-transcriptional level (Khadrawy et al, 2020). Furthermore, in the in vitro culture of bovine ovarian tissue using quercetin supplementation maintain the integrity of the development of ovarian follicles in in vitro culture for 5 and 10 days and improved antioxidant potential after 10 days of culture (Cerezetti et al, 2021). In goat oocytes, quercetin was able to replace the antioxidant cysteamine in the in vitro maturation medium, as well as increased oocyte mitochondrial activity, ensuring greater cell viability and quality (A.…”

Section: Ferric Reducing Antioxidant Power (Frap)mentioning

confidence: 99%

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Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Guimarães,

Silva,

Barberino

et al. 2023

Sem. Ci. Agr.

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This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes.

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Section: Ferric Reducing Antioxidant Power (Frap)mentioning

confidence: 87%

Section: Ferric Reducing Antioxidant Power (Frap)mentioning

confidence: 99%

Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Guimarães,

Silva,

Barberino

et al. 2023

Sem. Ci. Agr.

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“…Furthermore, quercetin also promotes embryonic growth and reduces apoptosis, thus improving the effectiveness of assisted reproductive techniques (65). In contrast, research has shown that quercetin reduces apoptosis in sperm cells when the body›s oxygen supply decreases, thus protecting against varicocele-related damage to sperm production (66). Quercetin›s function is as an antioxidant in the mitochondrial respiratory chain and, therefore, affects the treatment of infertility in men.…”

Section: Discussionmentioning

confidence: 99%

Effects of Quercetin on Codeine-Induced Reproductive Hormone Disorder

Mohammed,

Osama Adnan,

Abdaulteef Salih

2025

Egyptian Journal of Veterinary Sciences

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T HE CURRENT research studies quercetin's potential to attenuate its effects on adult male rats' codeine-induced reproductive disorders. Twenty-four male adult rats were separated into four sets, consisting of six male adult rats in every set, and a 56-day treatment was obtained. General, the first set is control, and the second set (Q1) obtained quercetin using a gavage needle administered at a specific dosage (300 mg/kg/day). In contrast, the third set (Q2) obtained codeine(50mg/kg/day) using a gavage needle, and the fourth group (Q3) obtained quercetin and codeine until 56 days. As a result of exposure to codeine, the rats› blood concentrations of luteinizing hormone (L.H.) and testosterone (T.) decreased. Nevertheless, the administration of quercetin caused an elevation in the levels of (L.H.) plus (T.) hormones in the set (Q3) compared with the set (Q2). In group (Q2), there were changes in the testicle›s structure and tissue, mainly in the Sertoli cells. In addition, there was amyloid accumulation inside the seminiferous tubules; these cells were disarrangements and lacked germ cells. Meanwhile, rats given quercetin (Q1) showed improvement in testicular histopathological abnormalities. The present study›s findings, taking quercetin with codeine (Q3), demonstrated that quercetin consumption can attenuate the harmful influences of exposure to codeine on male reproductive function. In conclusion, this study investigated quercetin›s capability to protect rat testis from the histological damage that codeine causes by oxidative stress.

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“…Research related to biotechnologies such as in vitro culture of bovine preantral follicles have shown the use of different substances in culture medium to mimic the physiological environment of the ovary. Recent studies have included antioxidant substances such as alpha-lipoic acid and quercetin at different concentrations in the in vitro culture media of preantral follicles included in bovine ovarian fragments ( Bergamo et al, 2022 ; Cerezetti et al, 2021 ). The results are promising because the fragmentation of the ovary and changes in the environment could generate ROS and restrict the success of in vitro growth of preantral follicles.…”

Section: Discussionmentioning

confidence: 99%

Oxidative activity of corpus luteum and ovarian parenchyma in Bos taurus indicus heifers

Miguez González,

Cerezetti,

Bergamo

et al. 2023

Anim. Reprod.

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The aim of this study was to evaluate the oxidative stress in ovaries and corpus luteum (CL) of Bos taurus indicus females and the oxidant effect of CL in ovarian tissues in regions near, intermediate, or distant from it. Ovaries (n=12) of Nelore heifers (n=6) were collected from a slaughterhouse and fragmented. Experiment 1, each ovary was obtained from three fragments, resulting in 18 fragments of ovaries with CL (OV+CL) and another 18 fragments of ovaries without CL (OV-CL). Three fragments were generated from CL, totaling 18 CL fragments. In experiment 2, the ovarian fragments were removed from specific regions near, intermediate, or distant from the CL. All the fragments were placed in Eppendorf -type microtubes (1 mL), kept in a thermal container at 4 ºC, and then stored in a -80 ºC freezer for analysis of oxidative stress (TBARS and NBT) and antioxidant potential (FRAP and ABTS). In the antioxidant activity analysis, luteal tissues showed more antioxidant activity than ovarian tissue (FRAP = P < 0.0001; ABTS = P < 0.02). In the oxidative stress analysis, CL had lower levels of reactive oxygen species (ROS; TBARS = P < 0.03; NBT = P < 0.0001) than ovarian tissues. There was no difference in antioxidant activity and oxidative stress between the fragments obtained from different regions (OV+CL versus OV-CL; P > 0.05). The presence of CL in the ovaries of Bos taurus indicus females did not influence the oxidative stress or antioxidant potential of the gonad. Thus, the removal of ovarian fragments with or without the presence of CL indicates that biotechnologies such as in vitro follicle cultivation is possible.

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Impact of the antioxidant quercetin on morphological integrity and follicular development in the in vitro culture of Bos indicus female ovarian fragments

Cited by 10 publications

References 37 publications

Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Effects of Quercetin on Codeine-Induced Reproductive Hormone Disorder

Oxidative activity of corpus luteum and ovarian parenchyma in Bos taurus indicus heifers

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