Impact of the Brassica oleracea Genome on Breeding and Crop Improvement

Fidler, Justyna; Żyła, N.; Babula-Skowrońska, Danuta

doi:10.1007/978-3-030-31005-9_10

Cited by 3 publications

(2 citation statements)

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional breeding of B. oleracea varieties has led to significant improvement in productivity and quality [40,41], but to overcome the limitations of current breeding programs and meet the needs of an ever-growing population, the application of novel technologies is necessary. Many protoplast-based approaches have already been used in plant research including genome editing using CRISPR/Cas9 [4,25].…”

Section: Discussionmentioning

confidence: 99%

Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Brassica oleracea var. capitata L.) Cultivars

Stajič

2023

Plants

View full text Add to dashboard Cite

Protoplasts are a versatile tool in plant biotechnology since they can be used for basic biological studies as well as for breeding strategies based on genome editing. An efficient protoplast isolation protocol is essential for conducting protoplast-based studies. To optimize the protoplast isolation protocol in cabbage (Brassica oleracea var. capitata L.), different enzyme solutions were tested for the isolation of leaf mesophyll protoplasts. In our experiments, the combination of 0.5% Cellulase Onozuka RS and 0.1% Macerozyme R-10 showed the best result. The optimized protocol proved suitable for the isolation of protoplasts from five different cabbage cultivars with yields ranging from 2.38 to 4.63 × 106 protoplasts/g fresh weight (fw) and a viability of 93% or more. After three weeks in culture, protoplasts from all of the tested cultivars formed micro-calli, but further callus growth and shoot regeneration depended strongly on the genotype and regeneration protocol used. For shoot formation, 1 mg/L BAP in combination with auxin 0.2 mg/L NAA showed the best results with a regeneration of 23.5%. The results obtained will contribute to the development of different applications of cabbage protoplasts and facilitate the breeding process of this important horticultural crop.

show abstract

Section: Discussionmentioning

confidence: 99%

Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Brassica oleracea var. capitata L.) Cultivars

Stajič

2023

Plants

View full text Add to dashboard Cite

show abstract

“…Example of crops with distinct morphology as a result of brassica oleracea domestication (Petruzzello, 2022) Approximately 76 million tons of Brassica vegetables were produced with a value of 14.85 billion dollars in 2010 (http://faostat.fao.org/) (Liu et al, 2014). Many Brassica crops have great nutritious value as they are rich in protein, vitamins, minerals, flavonoids and other elements essential for human health (Fidler et al, 2021).…”

Section: A Focus On Xanthomonas Campestris Pathovar Campestris the Di...mentioning

confidence: 99%

Arabidopsis and Xanthomonas campestris pv. campestris two model organisms in plant pathology

TEME

View full text Add to dashboard Cite

Causative agent of black rot of crucifers, Xanthomonas campestris pv. campestris (Xcc), is a rod-shaped Gram-negative bacterium that belongs to the [gamma]-subdivision of Proteobacteria. It was first described in 1889 by the entomologist and botanist Harrison Garman in Kentucky, USA. Xcc is nowadays one of the most economically damaging bacterial pathogens to plants of the family Brassicaceae. In addition, some accessions of the model plant Arabidopsis thaliana respond differently when infected with this bacterium. In this study, we investigate different responses of Arabidopsis ecotypes to Xanthomonas campestris pv. campestris. Two resistant ecotypes Drahonin (Dra-0) and Wassilewskija (Ws-2)) were identified using colony forming unit assay. Bacterial pathogens depend on different strategies (e.g., secretion systems) to invade and colonize their hosts. The pathogenicity of Xcc is correlated with the type 3 secretion system (T3SS) which is a protein appendage used by bacteria to transfer effector proteins into the host cells for virulence. Xanthomonas T3SS effector genes are classified into different groups called Xanthomonas Outer Proteins (Xops). xopAC is a major avirulence gene of Xanthomonas campestris pv. campestris in Arabidopsis ecotype Columbia 0. The second part of the research aims to study the interaction between Xanthomonas campestris pv. campestris 2669[delta]xopAC and different Arabidopsis mutants. Bioluminescence-based quantitative detection of bacteria in plants and colony counting assay are performed and compared to determine which method gives more accuracy and is less time consuming. The software ImageJ used to quantify the bioluminescence images was not as precise as expected. The transposon-based bioluminescence for quantitative detection of bacterial titer in plants was also successfully applied to the bacterium Pseudomonas syringae pv. tomato.

show abstract

Advancing Crop Management: A Deep Learning Paradigm for Automated Broccoli Club Root Disease Identification

Sharma,

Grover

2024

2024 International Conference on Cybernation and Computation (CYBERCOM)

View full text Add to dashboard Cite

Impact of the Brassica oleracea Genome on Breeding and Crop Improvement

Cited by 3 publications

References 137 publications

Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Brassica oleracea var. capitata L.) Cultivars

Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Brassica oleracea var. capitata L.) Cultivars

Arabidopsis and Xanthomonas campestris pv. campestris two model organisms in plant pathology

Advancing Crop Management: A Deep Learning Paradigm for Automated Broccoli Club Root Disease Identification

Contact Info

Product

Resources

About