DC characteristics of AlGaN/GaN HEMTs with different thickness values of the undoped GaN channel layer were compared. An abnormal transconductance (g m ) overshoot accompanied by a negative threshold voltage (V TH ) shift was observed during I DS -V GS sweep in devices with thinner GaN layer. At the same time, a non-monotonic increase in gate current was observed. In OFF-state, electron trapping occurs in the undoped GaN layer or at the GaN/AlN interface, leading to a positive V TH shift. When the device is turning on at a sufficiently high V DS , electron de-trapping occurs due to trap impact-ionization; consequently, V TH and therefore I D suddenly recovers, leading to the g m overshoot effect. These effects are attributed to electron trap impact-ionization and consequent modulation of the device's electric field.